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Drug composition for preventing and curing diabetes mellitus

A composition and diabetes technology, applied in the field of compositions extracted from natural plants, can solve the problems of lack of significant medicinal effect, effective ingredients and mechanism of action, complex process route and high production cost

Active Publication Date: 2013-07-17
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although various existing Chinese patent medicines have a certain therapeutic effect on diabetes, the single-component preparations currently used clinically or the prescription decoctions prepared by doctors lack significant efficacy, clear active ingredients and mechanism of action, and the quality is controllable and reliable. Modern traditional Chinese medicine with convenient dosage form
Chinese invention patent ZL200510110763.2 discloses a composition for treating diabetes, which is composed of Anemarrhena anemarrhena, Cortex Phellodendri and cinnamon extracts. The route is more complicated

Method used

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  • Drug composition for preventing and curing diabetes mellitus
  • Drug composition for preventing and curing diabetes mellitus
  • Drug composition for preventing and curing diabetes mellitus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Embodiment 1 Determination of timosaponin A-III, B-II and B-III content

[0089] Column: Diamonsil TM C 18 (200mm×4.6mm, 5μm). Column temperature: 25°C, flow rate 1.0ml / min. ELSD detector, drift tube temperature 110°C, atomizing gas flow rate 2.6SPLM. The injection volume is 20 μl.

[0090] Mobile phase A: acetonitrile; mobile phase B: 0.5% formic acid aqueous solution; equilibrated with 30% mobile phase B, linear gradient elution in 30 minutes, and terminated with 90% mobile phase B.

[0091] Accurately weigh 25.0 mg each of timosaponin A-III, B-II and B-III standard substances, put them in a 10ml measuring bottle, add an appropriate amount of methanol, sonicate to dissolve, let cool, add methanol to dilute to the mark, shake well, Reconfigure into 1.0mg / ml timosaponin B-II, B-III reference substance solution and 1.0mg / ml timosaponin A-III reference substance solution, set aside.

[0092] Accurately weigh 25 mg of total saponins extract of Anemarrhena, put it in...

Embodiment 2

[0096] Embodiment 2 Determination of mangiferin content

[0097] Chromatographic column: Diamonsil from Dima Company R C 18 Column (250mm×4.6mm, 5μm); column temperature is 20°C.

[0098] Mobile phase: acetonitrile-water (15:85, v / v), (adjust pH=3.0 with phosphoric acid); flow rate: 1 ml / min; injection volume: 20 μL.

[0099] Detection wavelength: 254nm.

[0100] Precisely weigh 6.3mg of mangiferin reference substance, put it in a 25ml measuring bottle, add appropriate amount of methanol, sonicate to dissolve, let it cool, add methanol to dilute to the mark, shake well, and prepare 0.252mg / ml mangiferin reference substance solution, set aside .

[0101] Accurately weigh 10 mg of total flavonoids extract of Anemarrhena, put it in a 25ml volumetric flask, add an appropriate amount of methanol, sonicate to dissolve, let it cool, add methanol to dilute to the mark, shake well, and then dilute to an appropriate concentration, then it is total flavonoids of Anemarrhena Sample s...

Embodiment 3

[0105] Example 3 Determination of Berberine Content

[0106] High-performance liquid chromatography: Agilent 1100 system; Hangzhou British Spectrum chromatography workstation.

[0107] Column: Diamonsil TM C 18 (250mm×4.6mm, 5μm); the column temperature is 20°C; the injection volume is 20μl.

[0108] Mobile phase: water-acetonitrile-glacial acetic acid-triethylamine (67:33:0.3:0.8, v / v), detection wavelength: 345nm; flow rate: 1ml / min.

[0109] Accurately weigh 10.0 mg of berberine hydrochloride, put it in a 25 ml measuring bottle, add methanol to dissolve and make up to the mark, and configure it as a 400 μg / ml stock solution, accurately draw 0.5 ml of berberine stock solution, add methanol to dissolve and make up to 10 ml, Make 1ml of reference substance solution containing 20μg of berberine.

[0110] Accurately weigh 10 mg of Phellodendron Phellodendri extract, put it in a 25ml volumetric flask, add appropriate amount of methanol, sonicate to dissolve, let it cool, add ...

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Abstract

The invention discloses a drug compound for preventing and curing diabetes mellitus, which at least comprises two components including Timosaponin B-II and berberine. Preventing and curing effect can be improved by adding one or a plurality of components from mangiferin, Timosaponin A-III and Timosaponin B-III. The drug compound can also be compounded by anemarrhena extractive and golden cypress extractive, or can be directly extracted from anemarrhena and golden cypress. The drug compound has the advantages of obvious blood sugar reduction effect, and high effective component content, easy quality control. The preparation process of the drug compound is easy for industrialization, and can be applied as drugs to prevent and cure diabetes mellitus.

Description

technical field [0001] The invention relates to a composition extracted from natural plants, in particular to a composition extracted from Zhimu and Cortex Phellodendri, which is used for the prevention and treatment of diabetes. Background technique [0002] Diabetes Mellitus (DM) is a series of clinical syndromes caused by absolute or relative insufficiency of insulin in the body. The disease will also cause some complications during the development process, such as: hypoglycemia (Hypoglycemia), ketoacidosis (Ketoacidosis), nonketotic hyperosmolar coma (Nonketotic Hyperosmolar Coma), cardiovascular disease, chronic renal failure, Retinopathy, neuropathy and microvascular disease. There are three main categories of drugs commonly used in the treatment of diabetes: biological drugs, such as: insulin (Insulin); chemical drugs, such as: sulfonylureas (Sulfonylurea), biguanides (Biguanide), glitazones (Glitazone) and Chinese patent medicines kind. Due to the fact that there ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/8964A61K31/7048A61P3/10A61K31/4375A61K31/352
Inventor 黄成钢范明松唐意红李志雄孙兆林吴斌马春晖刘会会
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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