Alternaria nees fungus capable of producing paclitaxel

Abstract

The invention relates to an alternaria nees fungus capable of producing paclitaxel, which is taken as an anti-cancer new drug with special action mechanism and always gets attention of people since the advent; however, the content of the paclitaxel in natural taxus is very low, and the taxus resources are limited, thereby limiting the further development and the application of the paclitaxel. Thecomposition of the alternaria nees fungus is as follows: the fungus is separated from barks of the taxus, the barks collected in the field are brought back to a lab in a sterile manner, cut into blocks by using a sterile knife and planted on a water agar culture medium in a three-point manner, the reservation is carried out after growing out white mycelium, the fermentation, the thin-layer chromatography and the high performance liquid chromatography analysis prove that the fungus has the ability of producing the paclitaxel, the yeast is collected in CCTCC, and the collection number is M207165. The alternaria nees fungus is taken as a raw material of the anti-cancer new drug.

Description

Technical field: [0001] The invention relates to an Alternaria fungus capable of producing paclitaxel isolated by a bioengineering method. Background technique: [0002] Paclitaxel compounds are the general term for diterpenoids isolated from the taxus genus Taxus, among which paclitaxel is particularly eye-catching. Other diterpenoids generally also have the paclitaxel mother nucleus structure, and some are paclitaxel biosynthesis and can be artificially synthesized into new compounds with medicinal value. Paclitaxel can bind to cellular tubulin, prevent microtubule depolymerization, and effectively control the rapid division and proliferation of cancer cells. As a unique mechanism of action The new anti-cancer drug has always been paid attention to by people since it came out, but due to the low content of paclitaxel in natural yew and the limited resources of yew, the further development and application of paclitaxel is limited. In order to solve this problem, recently O...

AI Technical Summary

Problems solved by technology

[0002] Paclitaxel compounds are the general term for diterpenoids isolated from the taxus genus Taxus, among which paclitaxel is particularly eye-catching. Other diterpenoids generally also have the paclitaxel mother nucleus structure, and some are paclitaxel biosynthesis and can be artificially synthesized into new compounds with medicinal value. Paclitaxel can bind to cellular tubulin, prevent microtubule depolymerization, and effectively control the rapid division and proliferation of cancer cells. As a unique mechanism of action The new anti-cancer drug has always been paid attention to by people since it came out, but due to the low content of paclitaxel in natural yew and the limited resources of yew, the further development and application of paclitaxel is limited. In order to solve this problem, recently Over the years, scientists have done a lot of work on finding and expanding the source of paclitaxel, including chemical synthesis, biotechnology and fermentation production using symbiotic bacteria, etc. The production of paclitaxel has very huge economic benefits and broad development space