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Method for extracting ceramide amphoteric lipid from agriculture-forestry byproducts

A technology for ceramides and by-products, which is applied in chemical instruments and methods, separation/purification of carboxylic acid amides, preparation of sugar derivatives, etc., can solve the problems of cumbersome steps, high impurities in ceramides, and low extraction rate, and achieves simplified separation. Steps, the effect of improving the extraction rate

Inactive Publication Date: 2012-11-28
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing extraction method has low extraction rate, cumbersome steps, and high impurities in the lipid of ceramide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Step 1: The corn processing waste is dried and crushed with a mesh size of less than 1mm. The treated agricultural and forestry by-products are placed in a reactor, mixed with water according to the corn processing waste:water weight ratio of 1:5-20, and the pH value of the extract is adjusted to 7.5-8.5 with sodium carbonate. 0.01% to 0.1% of amino acid chemical penetrant equivalent to the weight of agricultural and forestry by-products is added.

[0022] Step 2: Weigh protease equivalent to 0.1% to 1.0% of corn processing waste, slowly add to the above extract, and continue stirring for 10h to 24h.

[0023] Step 3: Then the extract is rotatably evaporated to dryness, and a mixed solvent with a volume ratio of chloroform:methanol of 9 to 2:1 is added thereto for extraction three times, and the volume ratio of the extract to the mixed solvent is 4 to 1:1. After the layers were separated, the organic layer was rotovaped to obtain lipid 1 as a concentrated solution.

[...

Embodiment 2

[0028] According to the preparation steps and method of Example 1.

[0029] 100 grams of processed wheat bran, the pulverized particle size of which is less than 1 mm, is placed in 2000 ml of water, and the pH value of the extract is adjusted to 8 with sodium carbonate. Glycine chemical penetration agent is added by 0.06% of the weight of wheat bran. Weigh 0.1% cellulase equivalent to the weight of rice bran, slowly add to the extract, and continue to stir for 24 hours. Then the extract was evaporated to dryness by rotary evaporation, and 2000 ml of mixed solvent of chloroform:methanol (4:1 by volume) was added thereto for extraction three times. After the layers were separated, the organic layer was rotovaped to obtain lipid 1 as a concentrate. Ceramide-rich lipid 1 was dissolved in dichloromethane, separated by macroporous resin column chromatography, and ceramide-rich lipid 2 was obtained by solvent elution. Ceramide-rich lipid 2 was added to 100 mL of 0.35 mol / L sodium ...

Embodiment 3

[0031] According to the preparation steps and method of Example 1.

[0032] 100 grams of processed rice bran, the crushed particle size of which is less than 1 mm, is placed in 1000 ml of water, and the pH value of the extract is adjusted to 7.5 with sodium carbonate. Add alanine chemical penetrant by 0.1% of rice bran weight. Weigh 0.8% papain equivalent to the weight of rice bran, slowly add to the extract, and continue to stir for 14 hours. Then the extract was evaporated to dryness, and 1500 ml of chloroform:methanol (volume ratio: 2:1) mixed solvent was added thereto for extraction three times. After separation, the organic layer was rotovaped to obtain Lipid 1 as a concentrate. Ceramide-rich lipid 1 was dissolved in a small amount of dichloromethane, separated by silica gel column chromatography, and ceramide-rich lipid 2 was obtained by solvent elution. Ceramide-rich lipid 2 was added to 150 mL of 0.15 mol / L sodium methoxide solution, and stirred at room temperature ...

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Abstract

The invention relates to a method for extracting ceramide amphoteric lipid from agriculture-forestry byproducts, which comprises the following steps: using waste materials of the processing of the agriculture-forestry byproducts of rice bran, wheat bran and corn as raw materials; adding protease which is 0.1 to 1.0 percent of the agriculture-forestry byproducts in weight and an amino acid chemical penetrating agent which is 0.01 to 0.1 percent of the agriculture-forestry byproducts in weight to the raw materials; extracting the raw materials for 3 times by using a mixed solvent of chloroform and methanol; performing column chromatographic separation; using a solvent to elute out lipid rich in ceramide; and after alkaline treatment, performing high-efficiency liquid phase chromatographic separation to obtain ceramide. The method has high extraction efficiency, simplifies the separation steps of other components, and obtains the ceramide lipid with different contents at the same time.

Description

technical field [0001] The invention relates to a method for extracting ceramide active amphoteric lipids in agricultural and forestry by-products, and relates to the field of effective preparation of active compounds from agricultural and forestry by-products through biological and chemical methods. Background technique [0002] Biological resources such as agricultural and forestry by-products are renewable resources, which are inexhaustible and have broad development prospects. China is the largest rice-producing country in the world, with an annual output of about 200 million tons of rice. Rice bran is a by-product of rice processing, accounting for about 5%-5.5% of the quality of rice. 1 / 3. Wheat bran is the main by-product of wheat flour processing. Generally, the bran produced by flour mills accounts for about 20% of the mass of wheat grains. The bran after wheat processing is basically directly used in the feed industry, and is rarely used for further processing an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C233/20C07C231/24C07H1/08C07H15/10
Inventor 崔艳丽王彩霞俞永平毛建卫
Owner ZHEJIANG UNIV
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