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Antigen detection method and application thereof

An antigen detection and antigen technology, applied in the field of diagnosis, can solve the problems of high detection cost and complicated operation

Inactive Publication Date: 2009-10-28
SHENZHEN PEOPLES HOSPITAL
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Problems solved by technology

(Georganopoulou DG, Lei Chang, Nam JM, et al. Nano particle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease. PNAS, 2005, 102(7): 2273-2276) This method currently only exists and experiments In the research stage, the antigen samples used are standard samples, and serum samples are required for clinical testing, so the dilution of serum needs to be studied. In addition, the method is complicated to operate and needs to be tested by computer chip technology, and the cost of testing is relatively high.

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  • Antigen detection method and application thereof
  • Antigen detection method and application thereof
  • Antigen detection method and application thereof

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Embodiment Construction

[0105] The invention combines nanotechnology and immunological detection technology, cleverly combines immunomagnetic microspheres and nano gold probes, uses nucleic acid barcode method to detect antigens, and converts the detection of trace protein antigens into the detection of nucleic acid barcodes.

[0106] The detection of nucleic acid barcode fragments that can be used in the present invention is not particularly limited, representative examples include (but not limited to): enzyme amplification detection method (biotin streptavidin), fluorescence quantitative method, nucleic acid chip detection method, etc.

[0107] The recognition molecules that can be used in the present invention are not particularly limited, and representative examples include (but are not limited to): antibodies (recognize antigens), antigens (recognized by antibodies), receptors (recognize ligands), ligands (recognize receptors) Wait.

[0108] The invention provides a method for detecting a trace ...

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Abstract

The invention relates to an antigen detection method and application thereof. The method converts detection of an antigen signal into detection of a nucleic acid barcode label, and comprises the following operation steps: (1) labeling modified nano magnetic microspheres by using an antibody I of an antigen to be detected to prepare immune magnetic microspheres, and labeling nano-gold sol by using an antibody II of the antigen to be detected and a nucleic acid barcode fragment at the same time to prepare a double-labeled nano-gold probe; (2) fully combining the immune magnetic microspheres, the nano-gold probe and the antigen to be detected, removing combined aggregate, and leaving supernatant fluid for detection; and (3) detecting the nucleic acid barcode label in the supernatant fluid. The antigen detection method can be applied to the detection of clinical PSA antigen. The method has the advantages of simple operation, high sensitivity, good specificity, easy automation and wide application prospect.

Description

【Technical field】 [0001] The invention belongs to the technical field of diagnosis, and in particular relates to an ultra-trace antigen detection technology and its application in the ultra-trace tumor antigen-prostate specific antigen (PSA) detection. 【Background technique】 [0002] With the rapid development of medical diagnostic technology, antigen detection methods are also changing with each passing day. Up to now, the three technologies of radioimmunoassay, enzyme-linked immunoassay and fluorescent immunoassay are the most widely used routine antigen molecular detection methods in immunochemistry, immunology and molecular biology. [0003] In 1959, American scholars Berson and Yalow established the radioimmunoassay (Radio Immunoassay, RIA) method. Because RIA has the advantages of high sensitivity, strong specificity, and simplicity, RIA has certain vitality, but because it must use radionuclides, it needs protection. , to prevent pollution; the effective use time of ...

Claims

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Application Information

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IPC IPC(8): G01N33/543C12Q1/68
Inventor 李富荣孔小丽齐晖
Owner SHENZHEN PEOPLES HOSPITAL
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