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Lactobacilus fermentum and method for preparing D-tagatose by utilizing Lactobacilus fermentum

A technology for fermenting lactobacillus and tagatose, which is applied in the fields of fermentation engineering and enzyme engineering, can solve problems such as low conversion rate, and achieve the effects of short fermentation period, convenient and simple operation, and short conversion time.

Inactive Publication Date: 2009-09-16
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wherein lactic acid bacteria are probiotics, the pH suitable for growth is consistent with the pH of the substrate lactose hydrolyzate, and is easy to operate, so it attracts attention. There are some patent applications, such as Lactobacillusplantarum (CN1948500A, 2007), L.pentose (US6057135, 2000), but The resulting product will be further metabolized in the later stage of conversion, resulting in a lower conversion rate

Method used

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  • Lactobacilus fermentum and method for preparing D-tagatose by utilizing Lactobacilus fermentum
  • Lactobacilus fermentum and method for preparing D-tagatose by utilizing Lactobacilus fermentum

Examples

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Embodiment 1

[0034] Incline medium: glucose 15g / L, peptone 10g / L, yeast extract 10g / L, CaCO 3 10g / L, agar 20g / L, pH6.2~6.4.

[0035] Seed medium: glucose 20g / L, peptone 10g / L, yeast extract 5g / L, diammonium hydrogen citrate 2g / L, sodium acetate 5g / L, K 2 HPO 4 2g / L, MgSO 4 0.58g / L, MnSO 4 0.25g / L, pH6.2~6.4.

[0036] Fermentation medium: glucose 20g / L, L-arabinose 5g / L, peptone 10g / L, yeast extract 5g / L, sodium acetate 10g / L, K 3 PO 4 0.2g / L, NaCl 0.01g / L, MgSO 4 0.2g / L, MnSO 4 0.1g / L, CaCO 3 10g / L, pH6.8~7.0, sterilized at 121℃ for 15 minutes.

[0037] Inoculate two rings of Lactobacillus fermentum NXTag-1 with the preservation number CGMCC No.2921 in the seed medium, cultivate at 37°C for 15 hours to obtain the seed liquid, and inoculate the seed liquid according to the inoculation amount of 3% of the volume of the fermentation liquid After cooling, in the fermentation medium, cultivate in aerobic conditions at 37°C for 14 hours to obtain a bacterial cell content of 16g / ...

Embodiment 2

[0039] The formula of the culture medium is the same as in Example 1, the seed liquid is inserted into the fermentation medium fermentation liquid (anaerobic device) according to the inoculation amount of 3% of the fermentation liquid volume, and cultured at 37° C. for 14 hours to obtain a thalline content of 10 g in the fermentation liquid / L, the enzyme activity reaches 1.45U / mL, cells containing L-arabinose isomerase are obtained by microfiltration, and 50mL of 90g / L D-galactose (containing 0.01mMMn 2+ ) solution, transformed at 60°C for 13h, and the D-tagatose in the transformation solution was detected to be 30.34g / L, and the transformation rate was 37.9%.

Embodiment 3

[0041] With 15g / L D-glucose as the carbon source, 5g / L L-arabinose as the inducer, 10g / L peptone and 5g / L yeast extract as the nitrogen source, the formula of the medium and the culture method are the same as in Example 1, and the seed liquid Insert the inoculation amount of 3% of the volume of the fermentation broth into the fermentation medium, and culture it aerobically at 37°C for 14 hours to obtain a bacterial cell content of 20g / L in the fermentation broth and an enzyme activity of 3.56U / mL, and obtain L-arabinose by ultrafiltration Add 1g / L of D-galactose (containing 0.01mM Mn 2+ ) solution, transformed at 60° C. for 15 h, the concentration of D-tagatose in the detected transformation solution was 5.6 g / L, and the transformation rate was 56%.

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Abstract

The invention discloses Lactobacilus fermentum, which is classified and named (Lactobacilus fermentum) NXTag-1 and is preserved in China General Microbiological Culture Collection Center, wherein a preservation number of the Lactobacilus fermentum is CGMCC No.2921. The invention also discloses a method for preparing D-tagatose by utilizing the Lactobacilus fermentum, namely a strain is grafted in a sterile culture medium containing a carbon source, a nitrogen source, inorganic salt and water, cells containing L-arabinose isomerase are obtained through the culture, and further D-galactose is transformed into the D-tagatose by adopting the dissociative or immobilized cells. The Lactobacilus fermentum can efficiently transform the D-galactose into the D-tagatose, the concentration of the D-tagatose in a transformation liquid can reach 45g / L, the transformation ratio of the D-tagatose to a substrate can reach more than 50 percent, and no side product generates.

Description

technical field [0001] The invention belongs to the technical fields of fermentation engineering and enzyme engineering, and in particular relates to a fermenting lactobacillus and a method for preparing D-tagatose by using it. Background technique [0002] D-tagatose (D-tagatose) is a relatively rare natural six-carbon ketose, the sweetness is 92% of sucrose, and the heat produced is only 1 / 3 of sucrose, which can improve intestinal flora and prevent dental caries And it has physiological effects such as auxiliary effect on curing type II diabetes patients, and is a good low-energy food sweetener and filler. In addition, because of its strong antioxidant and cell-protecting effects, D-Tag can be used as a valuable drug intermediate to significantly inhibit the toxicity of cocaine to liver cells and promote the health of the mother and embryo. D-Tag will be widely used in cosmetics , food and pharmaceutical industries. [0003] The development of tagatose began in the mid-...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/02C12R1/225
Inventor 徐虹汪芙蓉李莎欧阳平凯
Owner NANJING UNIV OF TECH
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