Polysaccharide with antitumor and immunologic adjuvant function as well as preparation method and purpose thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
An immune adjuvant and anti-tumor technology, applied in anti-tumor drugs, medical preparations containing active ingredients, pharmaceutical formulations, etc., can solve problems such as patient damage, achieve low toxicity, good safety, and improve spleen cell proliferation response. Effect
Inactive Publication Date: 2012-06-20
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] In the past, people put more emphasis on eradicating tumors, but in practice, cancer cells are often killed, and the patient is dying. The damage caused to the patient is irreversible.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0041] Embodiment 1: the preparation of polysaccharides of white vine pear
[0042] Preparation of total polysaccharides of Baiteng pear
[0043] The medicinal material Baiteng pear coarse powder 5kg was extracted by heating and refluxing with water for 2 hours, filtered, and the dregs were continuously extracted twice by refluxing and refluxing water. Combine the filtrates, recover under reduced pressure to 2000ml, add 3 times the volume of 95% ethanol with a mass concentration, mix well, and let stand at 4°C for 24 hours. The precipitate was collected and washed three times with ethanol, acetone and petroleum ether successively. The precipitate was dissolved in distilled water, dialyzed, and the retentate was concentrated under reduced pressure and freeze-dried to obtain the crude polysaccharide of Baiteng pear. The white vine pear crude polysaccharide is dissolved in 0.1mol / L sodium chloride, filtered through a 0.45 μm microporous membrane, purified on a DEAE-Sephadex A-5...
Embodiment 2
[0052] Embodiment 2: Anti-tumor effect in vivo of total polysaccharides of Baiteng pear
[0053] Take 50 clean-grade ICR mice, and subcutaneously inoculate each mouse with mouse sarcoma S 180Cell suspension 0.2ml. 24 hours after inoculation, they were weighed and randomly divided into groups of 10 rats. They were set as tumor-bearing control group, positive control group of cyclophosphamide (CTX), low, medium and high dose test groups of total polysaccharides of pear pear (AEPS). The tumor-bearing control group was orally administered normal saline at 0.2ml / 10g per day for 10 consecutive days; the positive control group was intraperitoneally injected with CTX at a dose of 20mg / kg, once every other day, a total of 5 times; Dosage groups were intragastrically administered at doses of 2.5 mg / kg, 5 mg / kg and 10 mg / kg, once a day for 10 consecutive times. The animals were sacrificed on the 11th day, the tumor tissues were taken out, weighed, and the tumor inhibition rate was cal...
Embodiment 3
[0058] Example 3: Effects of Baiteng pear total polysaccharides on the immune function of tumor-bearing mice
[0059] Take 60 clean-grade ICR mice, except for 10 mice in the normal control group, each mouse was subcutaneously inoculated with mouse sarcoma S in the right armpit. 180 Cell suspension 0.2ml. 24 hours after inoculation, they were weighed and randomly divided into groups of 10 rats. They were set as normal control group, tumor-bearing control group, cyclophosphamide (CTX) positive control group, and low, medium and high dose test groups of total polysaccharides of pear pears (AEPS). The normal control group and the tumor-bearing control group were orally administered normal saline at 0.2ml / 10g per day for 10 consecutive days; the positive control group was intraperitoneally injected with CTX at a dose of 20mg / kg, once every other day, a total of 5 times; , medium and high dose groups were intragastrically administered at doses of 2.5 mg / kg, 5 mg / kg and 10 mg / kg, o...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to polysaccharide with antitumor and immunologic adjuvant function, a preparation method thereof, a medicine composite using the same as an active component, and an application oThe invention relates to polysaccharide with antitumor and immunologic adjuvant function, a preparation method thereof, a medicine composite using the same as an active component, and an application of the same in tumor treatment and adjuvant used as vaccine. The polysaccharide is kiwifruit polysaccharide A, kiwifruit polysaccharide B, kiwifruit polysaccharide C, kiwifruit polysaccharide D, and kif the same in tumor treatment and adjuvant used as vaccine. The polysaccharide is kiwifruit polysaccharide A, kiwifruit polysaccharide B, kiwifruit polysaccharide C, kiwifruit polysaccharide D, and kiwifruit total polysaccharide as a mixture containing the four kiwifruit polysaccharide, which are all extracted and separated from the Chinese medicinal herb of kiwifruit roots, and the polysaccharidewifruit total polysaccharide as a mixture containing the four kiwifruit polysaccharide, which are all extracted and separated from the Chinese medicinal herb of kiwifruit roots, and the polysaccharide has the remarkable antitumor and immunologic adjuvant function. The polysaccharides can obviously inhibit the growth of transplantation S180 caruncle and H22 liver cancer of a mouse, improve the breehas the remarkable antitumor and immunologic adjuvant function. The polysaccharides can obviously inhibit the growth of transplantation S180 caruncle and H22 liver cancer of a mouse, improve the breeder reaction of splenic cells of a tumor-bearing mice, the activity of NK cells and CTL, the ability of the splenic cells secreting IL-2 and IFN-Gamma, the level of tumor antigen specific antibodies Ider reaction of splenic cells of a tumor-bearing mice, the activity of NK cells and CTL, the ability of the splenic cells secreting IL-2 and IFN-Gamma, the level of tumor antigen specific antibodies IgG, IgG2a and IgG2b in blood serum of the tumor-bearing mouse, strengthen the humoral immunity and cell-mediated immunity response of an egg-albumin immunized mouse, induce the organism to produce Th1gG, IgG2a and IgG2b in blood serum of the tumor-bearing mouse, strengthen the humoral immunity and cell-mediated immunity response of an egg-albumin immunized mouse, induce the organism to produce Th1type and Th2 type immunity responses, and can be used as immunologic adjuvant of antineoplastic and tumor vaccine with immunity adjustment. type and Th2 type immunity responses, and can be used as immunologic adjuvant of antineoplastic and tumor vaccine with immunity adjustment.
Description
technical field [0001] The present invention relates to Baitengli polysaccharide A, Baitengli polysaccharide B, Baitengli polysaccharide C and Baitengli polysaccharide D which are extracted and separated from the Chinese herbal medicine Baitengli and have anti-tumor and immune adjuvant effects, and Baitengli polysaccharide containing these four polysaccharides Polysaccharides, their preparation methods, pharmaceutical compositions with the polysaccharides as active ingredients, and their applications in treating tumors and as vaccine adjuvants. Background technique [0002] Tumor is one of the most difficult diseases in the world today, and it is the second leading cause of death after cardiovascular disease, which seriously threatens people's life and health and brings huge pressure to society and families. The global antineoplastic drugs account for about 4.2% of the total sales of the drug market. Although the share is not high, the annual growth rate has remained at doub...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more