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Method for extracting ribonucleic acid from animal pancreata

A ribonucleic acid and pancreas technology, applied in the field of animal biological products, can solve the problems of harming workers, unfriendly operating conditions, long production cycle, etc. Effect

Inactive Publication Date: 2009-08-26
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional RNA preparation methods mostly use 3-volume calorimetric phenol extraction for 3 times or sodium chloride-sodium citrate method to prepare animal organ RNA. Difficult to recycle, easy to cause environmental pollution, unstable product quality, difficult to control pyrogens, and even harm staff
At present, the traditional method of preparing RNA from bovine spleen often uses hot phenol in the process, which may dissociate DNA from histones, resulting in excessive DNA content, and at the same time, the production cycle is too long, and the production efficiency is low; the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Extraction of RNA from Bovine Pancreas

[0024] The thawed semi-frozen bovine pancreas was used to remove fat and blood vessels with sterilized scissors. Accurately weigh the amount of raw materials, wash the cut raw materials three times with tap water and one time with distilled water, and set aside. First, carry out initial grinding, weigh the spleen after degreasing, and first grind it 3 times in the meat grinder, and put it into a clean material bucket. Then carry out fine grinding, put the ground bovine pancreas and purified water into the mashing tank according to the ratio of 1:0.5, add 8 grams of sodium benzoate per kilogram of pancreas, fix the mashing tank and start the motor, Mash for 3 minutes, then press 1:1 (pancreas: distilled water), jog twice, 10 seconds each time, to achieve a state of mixing. Put the crushed beef pancreas slurry in the loading hopper, grind it according to the operating procedures of the colloid mill to make a homogenate...

Embodiment 2

[0025] Example 2: Identification of extracted RNA yield and purity

[0026] Thaw 10 kg of semi-frozen bovine pancreas to remove fat and blood vessels with sterilized scissors. Accurately weigh the amount of raw materials, wash the cut raw materials three times with tap water and one time with distilled water, and set aside. First, carry out initial grinding, weigh the spleen after degreasing, and first grind it 3 times in the meat grinder, and put it into a clean material bucket. Then carry out fine grinding, put the crushed cow pancreas and purified water into the mashing cylinder at a ratio of 1:0.5, add 80 grams of sodium benzoate, fix the mashing cylinder and start the motor, start the motor, mash for 3 minutes, Then press 1:1 (pancreas: distilled water), jog twice, 10 seconds each time, to achieve a state of mixing. Put the crushed beef pancreas slurry in the loading hopper, grind it according to the operating procedures of the colloid mill to make a homogenate, and use...

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PUM

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Abstract

The invention provides a method for extracting ribonucleic acid from animal pancreata, which comprises the steps of crushing, grinding by a colloid grinder, wall breaking by a high pressure homogenizer, ultra-filtration, purification, and the like, wherein the ultra-filtration is performed by a hollow fiber column of which the interception molecular weight is less than 10,000 daltons so as to obtain a high-purity and high-yield RNA. The method can crush the animal pancreata sufficiently to prevent splitting action of an RNA enzyme to the RNA. Simultaneously, the method can sufficiently eliminate the impurities of fat, protein, glycogen, and the like and reduce the dosage of an organic reagent so as to reduce the production cost and save the time, and has wide application prospect.

Description

technical field [0001] The invention belongs to the field of animal biological products, in particular to an extraction method for extracting ribonucleic acid from animal pancreas. Background technique [0002] Ribonucleic acid (abbreviated as RNA, that is, Ribonucleic Acid), is a genetic information carrier that exists in biological cells and some viruses and viroids. RNA is a long chain molecule formed by condensation of ribonucleotides through phospholipid bonds. A ribonucleotide molecule consists of phosphate, ribose sugar and bases. There are four main bases in RNA, namely A adenine, G guanine, C cytosine, and U uracil. Among them, U (uracil) replaces T thymine in DNA and becomes the characteristic base of RNA. Unlike DNA, RNA is generally a single-stranded long molecule and does not form a double helix structure, but many RNAs also need to form a certain secondary structure or even a tertiary structure through the principle of base pairing to perform biological func...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H21/02C12N15/10
Inventor 刘菁李旭东苏建东
Owner TIANJIN RINGPU BIO TECH
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