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Nano artificial dura mater with multi functional-layers and preparation method thereof

An artificial dura mater and functional layer technology, applied in the field of biomedicine, can solve problems such as unfavorable large-scale industrial production and clinical promotion, limited mechanical properties of carbon fibers, degradation speed, and defects in degradation performance, and achieve good bio-histocompatibility. , No foreign body rejection, and the effect of preventing the spread of diseases

Active Publication Date: 2009-08-19
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] ①At present, autofascia reconstruction of dura mater is still the standard for clinical application, but the incidence of cerebrospinal fluid leakage and subcutaneous fluid in the early postoperative period is still high, which can easily lead to complications such as adhesions, which may cause non-healing or dysfunction of the donor site
[0005] ② Allogeneic and heterogeneous materials cannot completely avoid immune rejection, and the residual antigens will lead to different degrees of inflammatory reactions, resulting in fibrosis and brain adhesions
[0009] ⑥Different non-absorbable materials have their own shortcomings: for example, metals are not conducive to CT, MRI and radiological examination and treatment; Thick scars can be formed on the outside of the cloth, and the underside is easy to adhere to the brain; the carbon fiber itself has limited mechanical properties
[0010] ⑦ Existing absorbable artificial meninges have certain defects in degradation speed and degradation performance, and the scope of clinical application is limited
[0011] In short, there are deficiencies in the clinical application of artificial meninges at present, which are not conducive to large-scale industrial production and clinical promotion.

Method used

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  • Nano artificial dura mater with multi functional-layers and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Preparation of anti-adhesion layer: select hydrophobic L-polylactic acid and ε-caprolactone, the ratio of the two is 50:50, as a copolymerized polymer material, the number average molecular weight is 260000, soluble in hexafluoroisopropanol .

[0048] Add the above solution into the syringe of the electrospinning device, adjust the rate of the micro-injection pump to 5 ml / hour, adjust the voltage of the high-voltage generator to 30KV, adjust the receiving distance of the receiving device to 20 cm, and receive the fiber into a film-like structure .

[0049] Here, the average diameter of the fibers obtained by electrospinning is 300 nm. .

[0050] Turn off the electrostatic device.

[0051] (2) Preparation of the cell scaffold layer: the following scheme was selected in this embodiment: the mass ratio of polyethylene glycol to chondroitin sulfate was 70:30, and the mass fraction of the spinning solution was 9%.

[0052] Turn on the electrospinning, and continue to...

Embodiment 2

[0056] (1) Preparation of anti-adhesion layer: select hydrophobic L-polylactic acid and ε-caprolactone, the ratio of the two is 50:50, as a copolymerized polymer material, the number average molecular weight is 260000, soluble in hexafluoroisopropanol .

[0057] Add the above solution into the syringe of the electrospinning device, adjust the rate of the micro-injection pump to 5 ml / hour, adjust the voltage of the high-voltage generator to 30KV, adjust the receiving distance of the receiving device to 20 cm, and receive the fiber into a film-like structure .

[0058] Here, the average diameter of the fibers obtained by electrospinning is 300 nm. .

[0059] Turn off the electrostatic device.

[0060] (2) Preparation of the transition layer: the following scheme is selected in this embodiment: the mass ratio of polyurethane to hyaluronic acid is 70:30, and the mass fraction of spinning solution is 10%.

[0061] Turn on the electrospinning, and continue spinning the transitio...

Embodiment 3

[0069] Carry out dog animal experiment with the dura mater that embodiment 1 makes:

[0070] There are 5 experimental dogs, weighing 15-20KG, aged 1.5-2 years, male or female. Intramuscular injection of ketamine was used for general anesthesia, and after anesthesia and hair removal, the animals were placed on a special operating table in the abdominal recumbent position. Disinfect with 2% iodine and 75% alcohol. The center of the head of the animal was cut longitudinally. The periosteum was separated with a stripper, the double top skull plate was exposed, and the skull was ground with a high-speed drill, and the double top was used to form a bone window. Use small scissors to cut off a rectangular dura mater with a size of 3 cm × 3 cm on the top of both sides to create a top dura mater defect. Electrocautery was performed on the exposed brain surface to cause six lesions of 1mm×1mm in size. Trim the artificial meninges prepared in Example 1 of the present invention into r...

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Abstract

The invention provides a nano artificial dura mater with a plurality of functional layers, which comprises a structure with at least two layers; the layer facing the brain is a hydrophobic anti-adhesion electrospun layer; and the layer back to the brain is a hydrophilic nano cytoskeletal layer. The invention also provides a method for preparing the nano artificial dura mater, which comprises the following steps: preparing an electrospun solution of a hydrophobic polymer; preparing an electrospun solution of a hydrophilic polymer; preparing the prepared electrospun solution of the hydrophobic polymer into the hydrophobic anti-adhesion electrospun layer through static spinning; and preparing the hydrophilic nano cytoskeletal layer on the anti-adhesion electrospun layer from the prepared electrospun solution of the hydrophilic polymer through static spinning, receiving a membrane structure, and obtaining the nano artificial dura mater. The mechanical features of the nano artificial dura mater can meet the requirements on tensile strength and flexibility of adaptation diseases; and the nano artificial dura mater can be freely cut as required, and has wide application prospect.

Description

technical field [0001] The invention relates to a nano-artificial dura mater with multiple functional layers and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Dural defects are common in neurosurgery clinical work. Open craniocerebral injuries (industry, traffic, war, etc.), tumor erosion, congenital meningeal defects and other craniocerebral diseases can cause dural defects. The dural defect needs to be repaired in time to prevent cerebrospinal fluid from overflowing, to prevent the swelling of the brain and the compression of atmospheric pressure, otherwise it will endanger human life. Dural defects can also cause complications such as intracranial infection, brain adhesions, and subcutaneous fluid, often causing diseases such as epilepsy, headache, and brain dysfunction. [0003] Although there are many substitutes for dura mater, the materials for repairing dura mater can be divided into four categories: aut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61L27/22A61L27/20A61L27/18D01D5/00
Inventor 袁玉宇徐弢
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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