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Corneal midterm preservation solution and preparation method thereof

A technology for preserving liquid and cornea, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of affecting the visual function of patients, corneal opacity, decreased transparency, etc., and optimize the decellularization conditions and morphology of animal matrix. Good, simple ingredients

Inactive Publication Date: 2013-01-02
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Irreversible damage to the corneal endothelium will lead to corneal opacity and decreased transparency. However, for patients who need corneal transplantation, this will undoubtedly affect the recovery of visual function and the improvement of visual acuity

Method used

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  • Corneal midterm preservation solution and preparation method thereof
  • Corneal midterm preservation solution and preparation method thereof
  • Corneal midterm preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Based on 100ml of preservation solution, 84.5ml of MEM culture solution is used as the base culture solution, containing 10ml of fetal bovine serum, 0.1mmol of non-essential amino acids, 0.1mmol of sodium pyruvate, 0.1g of tobramycin, 25mmol of HEPES, and 2.5g of chondroitin sulfate. And low molecular dextran 1g, pH value is 7.2~7.4, osmotic pressure is 350~380mOsm / L.

[0032] Preparation method 1: Add 2.5g chondroitin sulfate, 1g low molecular weight dextran to MEM containing 10ml fetal bovine serum, 100mM non-essential amino acid 1ml, 100mM sodium pyruvate 1ml, 1M HEPES 2.5ml, 10% tobramycin 1ml and 84.5ml MEM Mix the culture medium evenly, adjust the pH value to 7.2-7.4, and the osmotic pressure to be 350-380 mOsm / L, filter and sterilize it through a 0.2 μm membrane, and store it at 4°C after 20ml subpackage to obtain the preservation solution. Sodium bicarbonate needs to be added during use, and the pH value is adjusted to 7.3 to 7.4. Each sample is 20ml, and the r...

Embodiment 2

[0035] Based on 100ml of preservation solution, 84.5ml of M199 culture solution is used as the base culture solution, containing 10ml of special grade fetal bovine serum, 0.1mmol of non-essential amino acids, 0.1mmol of sodium pyruvate, 0.1g of tobramycin, 25mmol of HEPES, etc., and can also contain Chondroitin sulfate 2.5g, low molecular weight dextran 1g, pH value is 7.2-7.4, osmotic pressure is 350-380mOsm / L.

[0036] Preparation method 1: Add 2.5g chondroitin sulfate, 1g low molecular weight dextran to 10ml special grade fetal bovine serum, 100mM non-essential amino acid 1ml, 100mM sodium pyruvate 1ml, 1M HEPES 2.5ml, 10% tobramycin 1ml and 84.5ml Mix evenly in ml M199 culture medium, adjust pH to 7.2-7.4, osmotic pressure to 350-380mOsm / L, filter and sterilize through 0.2μm membrane, 20ml subpackage and store at 4°C to obtain this preservation solution. Sodium bicarbonate needs to be added during use, and the pH value is adjusted to 7.3 to 7.4. Each sample is 20ml, and t...

Embodiment 3

[0039] Based on 100ml of preservation solution, 42.25ml of MEM medium and 42.25ml of M199 medium were used as the base medium, containing 10ml of special grade fetal bovine serum, 0.1mmol of non-essential amino acids, 0.1mmol of sodium pyruvate, 0.1g of tobramycin, and 25mmol of HEPES etc., 2.5 g of chondroitin sulfate, 1 g of low-molecular-weight dextran, pH of 7.2 to 7.4, and osmotic pressure of 350 to 380 mOsm / L may also be included.

[0040] Preparation method 1: Add 2.5g chondroitin sulfate and 1g low molecular weight dextran to 10ml special grade fetal bovine serum, 100mM non-essential amino acid 1ml, 100mM sodium pyruvate 1ml, 1M HEPES 2.5ml, 10% tobramycin 1ml, 42.25ml Mix ml MEM medium and 42.25ml M199 medium evenly, adjust pH to 7.2-7.4, osmotic pressure to 350-380mOsm / L, filter and sterilize through 0.2μm membrane, and store 20ml in aliquots at 4°C. liquid. Sodium bicarbonate needs to be added during use, and the pH value is adjusted to 7.3 to 7.4. Each sample is ...

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Abstract

The present invention provides a liquid for preserving cornea for medium period and a preparing method thereof, and relates to a liquid for cornea material. The invention provides the liquid for preserving cornea for medium period and the preparing method thereof, wherein the liquid has the advantages of longer storing time, higher quality, no pollution and relatively lower cost compared with theprior liquid for preserving cornea for medium period. The liquid for preserving cornea for medium period contains culture solution, thickening agent, bovine serum, amino acid, antibiotic, acid-alkalimodifying agent and cell nutrition components. According to the formula of liquid for preserving cornea for medium period, the thickening agent, the bovine serum, the amino acid, the antibiotic and cell nutrition components are added into the culture solution for mixing to uniform. The pH value is adjusted to 7.2-7.4 with the acid-alkali modifying agent. The osmotic pressure is adjusted to 350-380mOsm / L with an osmotic pressure buffering agent. The liquid for preserving cornea for medium period is obtained after membrane filtering for sterilizing.

Description

technical field [0001] The present invention relates to a corneal material preservation solution, in particular to a method for treating corneal trauma, tumor, chemical burn (alkali burn), severe dry eye with vascularization, lack of limbal stem cells, immune diseases such as Stevens-Johnson syndrome ( SJS), ophthalmic pemphigoid, herpes simplex leukoplakia, corneal transplant rejection and other blinding eye diseases corneal intermediate preservation solution and preparation method thereof. Background technique [0002] According to the World Health Organization report, corneal disease is the second major cause of vision loss. New corneal blindness caused by corneal ulcers and eye trauma is 1.5 to 2 million every year. The main cause of childhood blindness is corneal leukoplakia caused by measles. . The only effective treatment for corneal blindness is a corneal transplant. However, due to the influence of religious beliefs and customs, there are very few people who donat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 刘祖国邹文进
Owner XIAMEN UNIV
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