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Method for immobilizing cell for producing Clostridium acetobutylicum

A technology of Clostridium butanicum and immobilized cells, which is applied in the direction of immobilization on or in the inorganic carrier, can solve the problem of high cost, and achieve the effects of high efficiency, long service life and easy degradation

Inactive Publication Date: 2009-07-08
青岛生物能源与过程研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some people use porous ceramics, plastics and other carriers as the immobilized carriers of solventogenic microorganisms. Although the cell enrichment effect is better, the cost is too high; The treatment of waste carriers in fixed beds after excessive growth and clogging has also become a difficult problem for industrial scale-up

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Clostridium beijerinckii ATCC 55025 was used as the solvent-producing strain, using RCM medium (beef extract 10g / l, peptone 10g / l, yeast extract 3g / l, glucose 5g / l, anhydrous sodium acetate 3g / l, chloride Sodium 5g / l) 37±1°C for 9 hours in an anaerobic incubator. The anaerobic environment is 80% nitrogen, 15% carbon dioxide, 5% hydrogen.

[0012] After removing the leaves and peeling the corn stalks harvested in the same year, cut them into 0.5cm pieces with a knife 2 Square small pieces, soaked in 0.2% (v / v) sulfuric acid solution for 10 minutes, washed 3 times with 2 times the volume of water, sterilized at 121°C for 15 minutes, then filled into the packed column of the reactor . Pass the RCM medium in the exponential growth phase into the packed column, and soak the carrier at 37±1°C for 12 hours, so that the free cells are automatically adsorbed on the surface of the corn stalk and enter the gap of the corn stalk. Continue to pass into the solvent-producing mediu...

Embodiment 2

[0014] Clostridium acetobutylicum ATCC824 was used as the solventogenic strain, and the exponential growth phase fermentation liquid was obtained by the method in Example 1. After the corn stalks were treated according to Example 1, they were chopped with a pulverizer and screened with a sieve with a volume of 0.05-0.1 cm 2 of pieces. After soaking with 1% (v / v) hydrochloric acid, cook at 121°C for 30min. After washing 3 times with 2 times the volume of water, dry in an oven at 80°C overnight to constant weight.

[0015] After the treated corn stalks were filled into the packed column, the Clostridium medium in the exponential growth phase was passed through for 0.5h -1 The dilution rate circulates through the packed column for adsorption fixation. After 48 hours of adsorption, pass through the solvent-producing medium as described in Example 1, and the dilution rate is 2h -1 . After 14 days of continuous operation of the reactor, the bacterial overgrowth reactor was bloc...

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PUM

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Abstract

A cell immobilization method of producing butanol clostridium adopts the butanol clostridium liquor at exponential growth phase to produce immobilized cells through the adsorption of straw. The method includes main steps as follows: A) crushing the corn stalk into small pieces, bathing in the dilute hydrochloric acid or dilute sulfuric acid at 80-100 DEG C for boiling 10-60 minutes, cleansing, and drying to a constant weight; B) immerging the corn stalk processed by the step A into a butanol clostridium cell culture fluid at exponential growth phase, static adsorption at 30 -40 DEG C for 12-48 hours. The inventive immobilization method has features of low cost, effective cell-rich effect, high activity of immobilized cells, and high production efficiency during continuous fermentation.

Description

technical field [0001] The invention relates to a cell immobilization method. The immobilized cells are Clostridium butanologenus, and the immobilized carrier is corn stalks. After the corn stalks are pretreated, the Clostridium culture medium in the exponential growth phase is directly adsorbed to obtain the immobilized cells. It can be used to ferment and produce acetone butanol. Background technique [0002] As important solvents, acetone and butanol have a wide range of uses. Since the 1950s, the acetone and butanol fermentation industry has been severely impacted by the oil industry, and the acetone and butanol fermentation industry has declined. With the gradual depletion of oil resources and the soaring price of crude oil in the world, the ancient acetone and butanol fermentation technology, as an effective biotransformation technology that uses biomass resources to produce fuel, has been recognized again by people, especially butanol Alcohol, as a new type of fuel ...

Claims

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Application Information

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IPC IPC(8): C12N11/14
Inventor 张跃冬马玉久杨芳晓张春辉
Owner 青岛生物能源与过程研究所
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