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Clump seedling of tissue culture of Saussurea involucrata Kar. et Kin. and large scale successive transfer culture method thereof

A subculture and tissue culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of consuming a lot of manpower, material resources, uneven growth state, inconvenient use and development, etc. The effect of stable quality, shortened production cycle and high output

Inactive Publication Date: 2009-07-08
DALIAN PRACTICAL BIOTECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The prior art shows that the induction and cultivation of snow lotus seedlings are mostly organogenesis. The problem of adopting this method is that a lot of manpower and material resources can be spent in the process of producing snow lotus seedlings, and the snow lotus seedlings grown from buds, The growth state is not uniform, which is not convenient for later utilization and development
There are many reports in the domestic literature on the large-scale subculture of Saussurea chinensis callus, and there are few reports on the direct cultivation of Saussurea chinense seedlings directly using the method of inheritance

Method used

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  • Clump seedling of tissue culture of Saussurea involucrata Kar. et Kin. and large scale successive transfer culture method thereof
  • Clump seedling of tissue culture of Saussurea involucrata Kar. et Kin. and large scale successive transfer culture method thereof
  • Clump seedling of tissue culture of Saussurea involucrata Kar. et Kin. and large scale successive transfer culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Obtaining of XCSY-1 callus of Saussurea chinensis

[0022] Take natural Xinjiang snow lotus seeds, soak them in 75% alcohol solution for 30 seconds, soak them in 0.1% mercuric solution for 10 minutes, wash them with sterile water for 4-5 times; Soak in 0.2mg / L solution for 24 hours; after soaking for 24 hours, soak the seeds again in 0.1% mercuric chloride solution for 10 minutes, wash with sterile water for 4-5 times; peel off the seeds under aseptic conditions Shell, tear off the inner membrane of the seed, and inoculate the peeled seed on the prepared MS medium (gibberellin 1.0mg / L-0.2mg / L) for plant induction, and sow 10 seeds per 40 milliliters of medium; Cultivate in the dark for 4-6 days at ℃, and after the seeds germinate, transfer the culture to light for 3-5 days to cultivate snow lotus plants.

[0023] Take aseptic seedling roots, stems, leaves, cotyledons, and embryos as explants, and under aseptic conditions, use sterile surgical scissors and ste...

Embodiment 2

[0026] Example 2 production method

[0027] 1 Production of bushy seedlings of snow lotus

[0028] 1.1 Selection

[0029] The snow lotus callus XCSY-1, which has been cultured for 10-14 days, has good growth, tender green or yellow-green color, compact structure, and obvious pointed protrusion tissue characteristics, is selected as the seed.

[0030] 1.2 Medium

[0031] MS improved medium ② (MS basic medium + 6-BA2.0-4.0mg / L + NAA0.1-1.0mg / L + sucrose 3% + agar 0.6%) 1.3 Inoculation

[0032] Under sterile conditions, pick the snow lotus callus XCSY-1, which is light green or yellow-green, compact in structure, and has the characteristics of obvious pointed protrusions, cut it into small pieces of tissue on a petri dish, and transfer the small pieces of tissue to In the cluster budding medium, the size of the inoculation block is 6-10mm.

[0033] 1.4 Culture of callus XCSY-1 of Saussurea chinensis

[0034] Cultivation temperature: 22-27°C, light 10h-12h, 2000lax; culture per...

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Abstract

The invention provides a method for tissue culture of cluster seedlings of herb saussureae involucratae in Xinjiang and a large-scale subculture method thereof. The method comprises the following steps: forming calluses on a MS modified culture medium (1) by induction; and inducing out a callus XCSY-1 which can quickly differentiate the cluster seedlings and stabilize a successive generation by screening and succession of the calluses on a MS modified culture medium (2), wherein, the callus can be quickly differentiated into the cluster seedlings with uniform state on a seedling strengthening culture medium. Numerous cluster seedlings of the herb saussureae involucratae with uniform growth state can be cultured by subculture proliferation of the callus XCSY-1 in Xinjiang. The cluster seedlings of the herb saussureae involucratae can be directly taken as product or raw materials, and can be cultured into rooting test tube seedlings for artificial plantation. The method has simple operation, can be applicable to large-scale production; and the cluster seedlings have more stable quality, higher yield and greatly shortened production cycle compared with wild cluster seedlings. In addition, the method requires less farmland, does not damage the wild herb saussureae involucratae resources, protects ecological environment, and helps solve the shortage problem of the herb saussureae involucratae resources in Xinjiang.

Description

technical field [0001] The present invention relates to rapidly differentiated clustered seedlings, stable, high-yield, subcultured Xinjiang Saussurea volata callus XCSY-1 and a selection method thereof. method. Background technique [0002] Saussurea involucrata (Saussurea involucrata Kar.et Kir.et Maxim.) is a herbaceous plant of the subgenus Saussurea involucrata in the family Asteraceae. Snow lotus is a folk medicinal plant in high mountain areas. It is used for dispelling cold and dehumidification, promoting blood circulation and stimulating menstruation, anti-inflammatory and analgesic, etc. It is mostly used in folk for rheumatoid arthritis, cold pain in women's lower abdomen, amenorrhea, retained placenta, impermeable numbness, and cold lung Treatment of cough, impotence, and altitude sickness. In recent years, Snow Lotus, as a national medicine, has attracted much attention for its anti-inflammatory and analgesic effects, anti-early pregnancy, anti-aging and inhib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 刘汉石
Owner DALIAN PRACTICAL BIOTECH
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