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Chitosan nano suspension and preparation method thereof

A nano-suspension, chitosan technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, metabolic diseases, etc., can solve the problems of application fields and bioavailability limitations, poor water solubility, large molecular weight, etc. To achieve the effect of easy control of reaction, simple operation and high product quality

Inactive Publication Date: 2009-06-17
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although chitosan has a variety of biological activities, it is limited in application fields and bioavailability due to its large molecular weight and poor water solubility.

Method used

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  • Chitosan nano suspension and preparation method thereof
  • Chitosan nano suspension and preparation method thereof
  • Chitosan nano suspension and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Refined chitosan (deacetylation degree DA is 92.5%, viscosity 315 mPas, provided by Shandong Jimo Chitosan Company) was placed in an oven at 80°C for 2 hours and cooled to produce 1.5% (W / L) acetic acid Solution, the concentration of acetic acid is 1.0%, dissolve at 25°C for 48 hours, take 200ml and put it in a 250ml normal saline bottle, use 60 The radiation dose of Co-γ rays is 25kGy (dynamic), and the dose rate is 0.7kGyh -1After irradiating, it was subjected to high pressure at 121°C for 2.5 hours, and then treated with ultrasonic waves. The ultrasonic intensity was 600W, the ultrasonic time was 3 seconds, and the interval was 2 seconds. Repeat 35 times for one ultrasonic unit, and repeat 10 ultrasonic units for about 35 minutes. Add 2.0% Tween-80 (final concentration), and mix well. The transmission electron microscope is magnified by 80000 times, and the physical film taken is processed by professional image analysis software (Image-proplus), and the calculation ...

Embodiment 2

[0038] Refined chitosan (same as in Example 1) was placed in an oven at 80°C for 2 hours, cooled, and made into a 2.0% (W) acetic acid solution, the concentration of acetic acid was 1.2%, dissolved at 25°C for 60 hours, and 200ml was divided into Packed in 250ml normal saline bottle, 60 The radiation dose of Co-γ rays is 30kGy (dynamic), and the dose rate is 0.83kGyh -1 , After irradiation, it was subjected to high pressure at 121°C for 2.5 hours, and then treated with ultrasonic waves, with an ultrasonic intensity of 600W, an ultrasonic time of 3 seconds, and an interval of 2 seconds. Repeat this 30 times for one ultrasonic unit, and repeat 10 ultrasonic units for about 30 minutes. Add 1.5% Tween-80 (final concentration), and mix well. Through the analysis and determination of dynamic light scattering instrument, average particle diameter=25.3nm, 99.1% chitosan nanoparticle particle diameter distributes between 19.9nm~31.5nm (see table 1 and image 3 ).

Embodiment 3

[0040] Refined chitosan (same as Example 1) was placed in an oven at 80°C for 2 hours, cooled to make a 3.0% (W) acetic acid solution, the concentration of acetic acid was 2.0%, dissolved at 25°C for 72 hours, and 200ml was subpackaged In 250ml normal saline bottle, 60 Coγ-ray irradiation, the dose is 50kGy (dynamic), the dose rate is 1.39kGyh -1 ,. After irradiating, it was subjected to high pressure at 121°C for 3.0 hours, and then treated with ultrasonic waves, the ultrasonic intensity was 800W, the ultrasonic time was 3 seconds, and the interval was 2 seconds. Repeat this 30 times as one ultrasonic unit, repeat 20 ultrasonic units, about 1 hour. Add 2.5% Tween-80 (final concentration), and mix well. After 80,000-fold magnification detection by transmission electron microscope, the statistical calculation result is: average particle diameter = 59.5nm, and the shape is spherical.

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Abstract

The present invention relates to a novel biological material, especially to a chitosan nanometer suspension which is used for aspects of health food, external-used medical dressing, etc. and has mutation-resisting biological efficiency, and a preparing method thereof. The chitosan nanometer suspension of the invention comprises nanometer chotosan, dissolvent, Tween-80 stabilizing agent, etc. According to the invention, an appropriate amount of chitosan is selected according to the mass indexes of deacetylation degree and viscosity. The chitosan is dissolved in acetic acid and is neutralized. The product is degraded through radiation of cobalt 60-gamma ray. The degradation product is executed with after-treatments of high temperature, high pressure, ultrasonic, etc. Finally the Tween-80 stabilizing agent is added and homogenized, and the chitosan nanometer suspension can be obtained. The method of the invention has the advantages of easy operation, low cost, controllable reaction, no pollution and high product quality. The chitosan nanometer particles with good equality can be obtained. The chitosan nanometer suspension can be used as health food, external-use medical dressing, etc. The biological material researching about other biological effect can be furthermore executed.

Description

technical field [0001] The invention relates to a biological material, especially a chitosan nano-suspension with anti-mutation biological effect for health food, external medical dressing and the like and a preparation method thereof. Background technique [0002] With the rapid development of industry and agriculture, chemical carcinogenesis has attracted more and more attention. According to the report of the World Health Organization, there are more than 5 million patients who die of cancer every year in the world, and the number will rise to more than 10 million at the beginning of the 21st century. , especially caused by chemical factors, including synthetic and natural compounds. At present, environmental pollution has become the number one killer of human survival and health. Many chemical substances have strong mutagenic effects and become environmental mutagens. Ames et al. reported that most carcinogens are mutagenic agents, and pointed out that carcinogens and ...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K9/10A61K31/722A61P31/00A61P35/00A61P3/06
Inventor 蒋玉燕房国坚毕忆群洪艳蒋建国陈勇
Owner ZHEJIANG ACAD OF MEDICAL SCI
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