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Preparation method of total flavone of Hovenia dulcisThunb

A technology of total flavonoids and Fructus Fructus, applied in the field of natural products, can solve the problems such as the lack of the preparation method of Fructus Fructus Fructus total flavonoids, the limitation of the utilization of Fructus Fructus Fructus and the medicinal effect.

Inactive Publication Date: 2009-01-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no literature report on the preparation method of total flavonoids of Hovenia dulcis, which seriously limits the utilization and efficacy of Hovenia dulcis flavonoids.

Method used

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  • Preparation method of total flavone of Hovenia dulcisThunb
  • Preparation method of total flavone of Hovenia dulcisThunb

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the preparation method of Hovenia dulcis total flavonoids

[0056] 1Kg of mature and dried Hovenia dulcis is crushed to 60 mesh, soaked in 60% ethanol solution (V / V) at a solid-to-liquid ratio of 1:7 (g / mL) for 60 minutes, controlled microwave power to 900W, and irradiated for 30 seconds. Cool to room temperature, then irradiate for 40 seconds, repeat the same operation 3 times, filter, and combine the extracts. The extract was concentrated to 20% (V / V) under reduced pressure, suspended in water, and then extracted with n-hexane and ethyl acetate in sequence; the ethyl acetate extract was filtered with a diatomaceous earth filter: first use No. 300 silica gel Pre-coat and sterilize the diatomite filter with diatomite and 90°C hot water, then pre-coat and sterilize the diatomite filter with No. 20 diatomite and 90°C hot water, and then use the diatomite extract to Filtrate obtained by filtering with a soil filter; vacuum concentrated and dried to obtain to...

Embodiment 2

[0057] Embodiment 2: the preparation method of total flavonoids of Hovenia dulcis

[0058] Get 1Kg of ripe and dry Hovenia dulcis seeds and pulverize them, pass through a 100-mesh sieve, add 7 times the amount (mL / g) of 70% ethanol solution (V / V), reflux extraction 3 times, each time for 2 hours, the extract is concentrated to remove ethanol, and It is suspended in water, extracted with petroleum ether to remove impurities such as pigments, and the aqueous phase extract is put on a LX-38 macroporous resin column, and the sample is slowly loaded, and the 4 column volumes are first eluted with water, and then 10% ethanol solution (V / V) Elution is 5 times of column volume, and finally eluted with 95% ethanol solution (V / V), when 10% AlCl 3 When the solution (W / V) is detected as negative, the collection is stopped, and the elution solvent is combined with 95% ethanol solution (V / V), concentrated and dried in vacuo to obtain the total flavonoids of Hovenia dulcis. Determination o...

Embodiment 3

[0059] Embodiment 3: the preparation method of Hovenia dulcis total flavonoids

[0060] Get 1Kg of dried pomace after removing the juice from the stalk of Hovenia dulcis, pulverize, pass through a 100-mesh sieve, filter out impurities such as cellulose, dry the powder, and use 8 times the amount (mL / g) of 70% ethanol solution (V / V) 80 Heat reflux extraction at ℃ for 3 times, 2 hours each time, combine the filtrates, concentrate in vacuo to remove ethanol, dissolve in distilled water, put on the AB-8 macroporous resin column, load the sample slowly, first elute with water and the outflow is nearly colorless, and then use 3 times Rinse the resin bed volume with 20% ethanol solution (V / V) to remove impurities, discard the rinse solution, elute with 60% ethanol solution (V / V) 7 times the volume of the resin bed, collect the eluate, concentrate to a thick paste and dry Obtain the total flavonoids of Hovenia dulcis. Determination of the content of total flavonoids is 73%, wherein q...

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Abstract

The invention discloses a method for preparing total flavonoids of hovenia acerba. The method adopts one optional method of the solvent extraction method, the solvent extraction method, the macro-porous resin adsorption method, the liquid-liquid countercurrent distribution chromatography, the supercritical fluid extraction method or the column chromatography, or the free combination of the methods. The total flavonoids of hovenia acerba is prepared from any one part of hovenia acerba, such as fruit stems, fruits, seeds, seed-cases, roots, stems, leaves, flowers, branches, skins, skin and fruit residues, etc., and the combination thereof. The prepared total flavonoids is a combination of various flavonoid active components, wherein the total flavonoids comprises the main active components as follows: quercetin, kaempferol, (+)-dihydromyricetin, myricetin, north hovenia acerba alcohol, (+)-pyrocatechin, hovenia acerba I, hovenia acerba II, hovenia acerba III and the derivates thereof; the total percentage composition of various flavonoid components by the weight is 5% to 100%; and the preparation method is applicable to pharmaceutical industry, food industry, cosmetic industry and health-care product industry.

Description

technical field [0001] The invention relates to a preparation method and a quality control method of Hovenia dulcis total flavonoids and its application in the fields of medicine, food, cosmetics and health care products, belonging to the field of natural products. Background technique [0002] Hovenia acerba Lindl (Hovenia acerba Lindl) is a plant of the genus Jujube in the family Rhamnaceae. It is also known as Hovenia acerba Lindl, Jujube, wood honey, Jujube, chicken feet, wild fruit, golden fruit, etc. It is a very precious fruit tree for both medicine and food. , mainly distributed in Shaanxi, Jiangxi, Anhui, Zhejiang, Guangdong, Fujian, Hubei, Hunan, Guangxi, Sichuan, Guizhou, Yunnan and other provinces, with extremely rich resources. The fruit or seeds with fleshy stalks are used as medicine, which is called "Hovenia dulcis", which has the functions of quenching thirst and relieving restlessness, detoxification of alcohol, diuresis, sobering up the nerves, dispelling ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/752A61K8/97A61P1/16A61P25/32A61P31/04A61P39/06A61Q19/00A23L1/30A61K131/00A61K125/00A61K127/00A61K133/00A61K135/00A23L33/105
Inventor 张存莉吴万兴师进霖
Owner NORTHWEST A & F UNIV
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