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Triterpene compounds separated from seed of cowherb of Chinese traditional medicine and uses thereof

A kind of Wang Bu Liuxing, compound technology, applied in the field of triterpenoids

Inactive Publication Date: 2008-12-31
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there have been researches on the anti-tumor effect of the medicinal materials of Buliuxing in vitro (In vitro anticancer activity of twelve Chinese medicinal herbs, Phytotheraphy research, 2005, 19(7): 649-651.), but for three of them Terpene saponins, there is no report in this regard

Method used

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  • Triterpene compounds separated from seed of cowherb of Chinese traditional medicine and uses thereof
  • Triterpene compounds separated from seed of cowherb of Chinese traditional medicine and uses thereof
  • Triterpene compounds separated from seed of cowherb of Chinese traditional medicine and uses thereof

Examples

Experimental program
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Embodiment 1

[0041] Select 5 kg of the processed wang buliu xing medicinal material, reflux extraction with 60 liters of 85% (V / V) industrial ethanol three times, each time for 2 hours, combine the extracts, and recover the ethanol by vacuum distillation to obtain 250 g of extract. After the extract is mixed with 300 grams of 100-200 mesh silica gel, it is eluted and separated by silica gel column chromatography with acetone and methanol respectively. Collect the methanol eluate, recover the solvent under reduced pressure until dry, and desugar through a D101 macroporous resin column to obtain 45 grams of extract. Get 2 grams of this extract and be mixed with 1 g / ml aqueous solution, adopt semi-preparative high-performance liquid chromatography (Inertsil ODS-3 column: 1.0 * 25 centimeters; 30-38% CH 3 CN-water (containing 0.6% trifluoroacetic acid) is the elution system; flow rate: 3.5 ml / min; UV detector (210 nm); injection volume: 200 microliters) for separation, collect the correspondin...

Embodiment 2

[0043] Select 5 kg of the processed wang buliu xing medicinal material, reflux extract with 40 liters of 60% (V / V) industrial ethanol three times, each time for 2 hours, combine the extracts, and recover the ethanol by vacuum distillation to obtain 260 g of extract. After the extract is mixed with 250 grams of 200-300 mesh silica gel, it is eluted and separated by silica gel column chromatography with acetone and methanol respectively. Collect the methanol eluent, recover the solvent under reduced pressure until dry, and desugar through a D101 macroporous resin column to obtain 50 grams of extract. Get 2 grams of this extract and be mixed with 1 g / ml aqueous solution, adopt semi-preparative high-performance liquid chromatography (Inertsil ODS-3 column: 1.0 * 25 centimeters; 60~65% CH 3 OH-water (containing 1.6% trifluoroacetic acid) elution system; flow rate: 1.5 ml / min; UV detector (210 nm); injection volume: 200 microliters) for separation, collect the corresponding fraction...

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Abstract

The invention discloses six triterpenoid compounds separated from the vaccaroside, namely, vaccaroside I, vaccaroside E, vaccaroside G, vaccaroside B, segetoside H and segetoside I. The in-vitro antitumor tests show that the compounds have obvious inhibitory effect on LNcap, P-388 and A-549 cell lines and provide a new ides on the development of new anti-cancer drugs and have great value on the deep development of traditional Chinese medicine resources.

Description

technical field [0001] The invention relates to the technical field of medicine, and more specifically relates to triterpenoids isolated from the traditional Chinese medicine wangbuliuxin and their medical application. Background technique [0002] Wangbuliu is the dry mature seed of Vaccaria segetalis (Neck.) Garcke, a plant of Caryophyllaceae. It has the effects of promoting blood circulation and promoting menstrual flow, reducing swelling in the breast. It is used for the treatment of lactation retention, amenorrhea, dysmenorrhea, mastitis swelling and pain. The plant is widespread in Asia, Europe and other parts of the world. Rich in a large number of triterpene saponins and cyclic peptide compounds with estrogenic activity. So far, triterpenoids have been shown to inhibit luteal cells (Segetoside F, a newtriterpenoid saponin with inhibition of luteal cell from the seeds of Vaccariasegetalis, Tetrahedron letters, 2000, 41(48): 9205-9207. ) and uterine contractility (...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/704A61P35/00
Inventor 黄成钢马春辉丁健楼丽广孙兆林唐意红李志雄范明松
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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