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Xingsiang Tuerfeng total phenolic acid extract and preparation method thereof

A technology for extracting Apricot fragrant rabbit ear wind and extract is applied in the field of total phenolic acid extract of Xingxiang fragrant rabbit ear wind and its preparation, and can solve problems such as loss and the like

Active Publication Date: 2008-12-31
BEIJING SUNHO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing the total phenolic acid extract of apricot rabbit ear wind. The present invention adopts ethanol extraction during extraction, and the recovered liquid after extraction is added with hydrochloric acid to adjust the pH under the situation of about 30% ethanol to make the phenolic acid Phenolic components are dissociated from it and dissolved in ethanol solution, which overcomes the defect that some relatively small phenolic acid components are not easy to dissolve in edema and lose with precipitation, and the final extract yield is high, and the phenolic acid in the extract The content also increased significantly

Method used

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  • Xingsiang Tuerfeng total phenolic acid extract and preparation method thereof
  • Xingsiang Tuerfeng total phenolic acid extract and preparation method thereof
  • Xingsiang Tuerfeng total phenolic acid extract and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Take 1000g of the dried medicinal material of Xingxiang rabbit ear wind, crush it into a coarse powder, add 10 times the amount of 40% ethanol to reflux extraction for 3 times, each time for 2 hours, filter, combine each extraction filtrate, concentrate under reduced pressure at 60°C, add ethanol to adjust Add it to the concentrated solution so that the final alcohol content of the drug solution is about 20%, and each milliliter of the drug solution is equivalent to about 1.0g of the original material of apricot rabbit ear wind, then add 1N hydrochloric acid to adjust the pH to about 2.0, and stir well , place at room temperature for 24 hours, centrifuge, take the supernatant, continue to concentrate the supernatant under reduced pressure to no alcohol, put the concentrate on the NKA-9 macroporous adsorption column that has been treated, and first elute it with deionized water to The color of the water eluent becomes lighter, and it is eluted with 60% ethanol, until FeCl...

Embodiment 2

[0036] Take 1000g of the dried medicinal material of rabbit ear wind, crush it into a coarse powder, add 12 times the amount of 60% ethanol to reflux extraction for 3 times, each time for 1 hour, filter, combine each extraction filtrate, concentrate under reduced pressure at 60°C, add ethanol to adjust to In the concentrated solution, the final alcohol content of the medicinal solution is about 40%, and each milliliter of the medicinal solution is equivalent to about 0.8g of the original medicinal material of Xingxiang rabbit ear wind, then add 1N hydrochloric acid to adjust the pH to about 1.5, stir well, and leave at room temperature Stand for 12 hours, centrifuge, take the supernatant, continue to concentrate the supernatant under reduced pressure to no alcohol, put the concentrated HP-20 type macroporous adsorption lipid column on the concentrate, first elute with deionized water until washed with water The deliquored color becomes lighter, and 70% ethanol is used for eluti...

Embodiment 3

[0038] Effects on chronic pelvic inflammatory disease in rats caused by Escherichia coli

[0039] 40 Wister rats were randomly divided into four groups, which were sham operation group, model group, Xingxiang rabbit ear wind total phenolic acid extract group and Fuke Qianjin tablet group, and the dosage of Xingxiang rabbit ear wind was 60mg / kg.d -1, administered once a day for 21 consecutive days, then the immune function of the rats was measured and the morphological observation of the rat uterus was carried out. -1 , the lectin titer was 85.8±1.7, compared with the model group (36.9±1.7), the rat serum lectin titer significantly increased, P<0.01; the lymphocyte transformation index of the extract administration group of the present invention was 1.6±1.7 0.6, compared with the model group 1.2±0.2, there was a significant difference, P<0.01, the lymphocyte transformation index was significantly improved.

[0040] Histomorphological observation found that the uterus in the mo...

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Abstract

The invention relates to an extract of frangrant ainsliaea herb total phenolic acids and the preparation method thereof, after the medicinal materials of frangrant ainsliaea herb are extracted by 70% ethanol through reflux, the ethanol extract is concentrated to small volume and the ethanol content in the solution is controlled to be 20% - 40%, after the pH is adjusted to be 1-3 by hydrochloric acid, the solution stands still and is filtered, then the solution is clear and concentrated, the material obtained is put on a macroporous absorption resin column and eluted by ethanol after washed, and then ethanol eluent is collected, concentrated and dried, thus the extract of the invention is obtained. The content of total phenolic acids in the extract can be up to over 60%, and the chlorogenic acid in the extract can be more than 3%. The extract of the invention has the effects of antiinflammatory action and can be used for preparing the drugs that can treat gynae phlegmasia.

Description

technical field [0001] The invention relates to a total phenolic acid extract of apricot and rabbit ear wind and a preparation method thereof, belonging to the field of preparation of active ingredients of natural medicines. Background technique [0002] Ainsliaea fragrans Champ. is a plant of the genus Ainsliaea fragrans Champ. It has the functions of clearing away heat and detoxification, promoting dampness, and stopping bleeding. The whole herb is used in folk medicine for the treatment of tuberculosis, hematemesis, bruises, nameless swelling and pain, Cavities, edema, chronic cervicitis and other diseases are recorded in the 1996 edition of "Jiangxi Provincial Standards of Chinese Medicinal Materials". It has been reported that it has inhibitory effects on Staphylococcus aureus, Streptococcus B, poliovirus, herpes simplex virus (HSV-1), and measles virus. [0003] Modern studies have shown that apricot rabbit ear wind medicinal materials contain phenols, flavonoids, org...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P15/00A61P31/04A61P31/12
Inventor 熊国裕
Owner BEIJING SUNHO PHARMA
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