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Method for producing spina date seed extract using complex enzyme

A technology of jujube seed extract and extract solution of jujube seed, which is applied in the field of producing jujube seed extract, can solve the problems of unsafe food, solvent residue, etc., and achieve the effects of food safety, stable results and good repeatability

Inactive Publication Date: 2008-12-17
TIANJIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing jujube seed saponin is extracted with organic reagents such as ethanol or n-butanol, and the extract is likely to have solvent residues, which is unsafe to eat

Method used

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  • Method for producing spina date seed extract using complex enzyme
  • Method for producing spina date seed extract using complex enzyme
  • Method for producing spina date seed extract using complex enzyme

Examples

Experimental program
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Embodiment 1

[0024] Pulverize the crude drug of Semen jujube Semen, pass through a 80-mesh sieve, take 20 g of Semen Semen Semen Semen Semen Semen Jujubee and degrease with petroleum ether to remove about 30% of the kernel fat oil to obtain about 13 g of Semen Semanthus Semen Semen Semen Semen Defat. Use enzymes according to 30U / g jujube seed crude drug respectively, add 600U cellulase and 600U pectinase, add distilled water to dissolve, and adjust the pH value to 4.5 with acetic acid to make an enzymolysis solution. The above enzymolysis solution was enzymatically hydrolyzed at 40° C. for 5 hours, and the pH value of the enzymolysis solution was kept at about 4.5 during the enzymolysis process. The above enzymolysis solution was refluxed twice for 1 hour each time. Filtrate and collect jujube seed saponin aqueous extract. Rotary evaporation of the above jujube seed saponin aqueous extract at 60-65° C., concentrated to a paste, to obtain a concentrated product. The above-mentioned concen...

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Abstract

The invention discloses a method for producing spine date seed extract by complex enzyme and aims at providing a method for producing a safe and edible spine date seed extract which is favorable for the stripping of the effective hypnotic-sedative component in jujuboside. The preparation method comprises the steps as follows: the spine date seed crude drug powder is defatted to obtain defatted spine date seed powder; the defatted spine date seed powder is mixed with cellulase and pectinase and added with water for dissolution, the pH value of the solution is adjusted to 4.5, enzymolysis of the solution is carried out at 40 DEG C for 5 hours, reflux water are extracted for twice, and rotary evaporation is carried out to obtain concentrate, the concentrate is dipped in 70% ethanol for 24 hours, evaporated in a rotary manner and condensed to obtain supernatant, the supernatant is dried in vacuum to obtain the crude extract of the spine date seed; the crude extract is attached to AB-8 macroporous absorbent resin and eluted by 30%-70% ethanol solution in a gradient manner, the eluent is collected, evaporated in a rotary manner, condensed and dried in vacuum to obtain an extract with jujuboside being equal to or more than 40%. The method adopts the complex enzyme of cellulase and pectinase to act on the spine date seed; the process conditions optimized are favorable for the stripping of the effective component. The spine date seed extract is safe in eating.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, relates to a method for producing jujube seed extract by using compound enzymes. Background technique [0002] During the extraction process of the jujube seed extract, when the active ingredients of the jujube seed in the cell protoplast diffuse to the extraction medium, the double resistance of the cell wall and the intercellular substance must be overcome. Existing jujuboside is extracted with organic reagents such as ethanol or n-butanol, and the extract is likely to have solvent residues and is unsafe to eat. Contents of the invention [0003] The purpose of the present invention is to overcome the deficiencies in the prior art, provide a dense structure capable of destroying the cell wall, reduce the mass transfer resistance of the cell wall, intercellular substance and other mass transfer barriers to the diffusion of active ingredients from the intracellular to the extract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P25/20C12P1/00
Inventor 张明春韩克勤解军波张彦青
Owner TIANJIN UNIV OF COMMERCE
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