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Method for preparing glyceride rich in conjugated linoleic acid in supercritical state by enzyme method

A technology for the preparation of conjugated linoleic acid glycerides and enzymatic methods, which is applied in the field of preparing glycerides rich in conjugated linoleic acid, which can solve the problems of low transesterification rate in acidolysis, improve solubility and increase enzyme reaction rate , High stability effect

Inactive Publication Date: 2010-10-27
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention is to solve the problem of low acidolysis transesterification exchange rate in the actual reaction, and proposes to use free lipase to catalyze the acidolysis transesterification reaction in a supercritical state

Method used

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Experimental program
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specific Embodiment approach 1

[0006] Specific embodiment one: use free lipase to catalyze the transesterification reaction in a supercritical state through the following steps: 1. Add CLA and triglycerides to the reactor in a ratio of 1:1 to 5:1, and then add 1 ~10% deionized water, add 5~15% free lipase, seal the reactor and fill it with CO 2 Air-tightly exchange two to three times to ensure complete replacement and removal of the air in the reactor, and at the same time put the reactor in water to test for leaks; 2. Fill the reactor so that the pressure in the reactor reaches 7.5MPa-25Mpa while ensuring the seal of the reactor. Place the reaction kettle in a constant temperature water bath, react at a temperature of 30-80°C and a rotation speed of 30rpm for 24-72 hours to obtain the acidolysis product; 3. Terminate the reaction, take the reaction kettle out of the water bath, and lower it to room temperature. Release CO in the kettle 2 To normal pressure, add sodium sulfate to terminate the reaction at ...

specific Embodiment approach 2

[0007] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in Step 1, CLA and triglycerides are added to the reactor at a ratio of 2:1 to 4:1. Other steps are the same as in the first embodiment.

specific Embodiment approach 3

[0008] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that in step 1, CLA and triglycerides are added to the reactor at a ratio of 1:1 to 5:1, and then 2 to 6% of ionized water. Other steps are the same as in the first embodiment.

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Abstract

The invention discloses a method for preparing glyceride rich in conjugated linoleic acid in a supercritical state by an enzyme method, and relates to the method for preparing the glyceride rich in the conjugated linoleic acid in the supercritical CO2 state by the enzyme method. The method for preparing the glyceride rich in the conjugated linoleic acid in the supercritical state by the enzyme method is implemented through the following steps of: 1, reactive pre-processing; 2, enzymatic acidolysis; 3, reaction termination; and 4, separation and purification; thus the glyceride rich in conjugated linoleic acid is obtained. Because the reactant has high solubility in the supercritical CO2 fluid and the solubility of lipase and conjugated linoleic acid in the glycerol trimester is increased, the enzyme is easy to catalyze the hydrolysis and the synthesis of the glycerol trimester and the yield is increased by 5 to 10 percent than that of a conventional method without adding any solvent by performing acidolysis interesterification on the glycerol trimester and the conjugated linoleic acid by the method.

Description

Technical field: [0001] The invention relates to a method for preparing glycerides rich in conjugated linoleic acid. Background technique: [0002] Conjugated linoleic acid (CLA) and its derivatives have many important physiological functions such as cancer suppression, weight loss, blood lipid regulation, blood sugar regulation, blood pressure regulation, anti-atherosclerosis, and immune regulation. The content of CLA in nature is relatively small, mainly in the fat of ruminant cattle and sheep and their dairy products. Plant food also contains CLA, but only 0.1mg-0.7mg CLA per gram in general vegetable oil, which cannot meet the needs of special people for CLA. demand. At present, CLA products appearing in the domestic and foreign markets are mainly in the form of free fatty acids. Free polyunsaturated fatty acids (PUFA) are easily oxidized to produce peroxides that are harmful to the human body, and have a sour taste and bad taste. It is difficult to be accepted by peopl...

Claims

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Application Information

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IPC IPC(8): C12P7/64
CPCY02P20/54
Inventor 江连洲王立琦于殿宇李默馨周小丹常云鹤刘晶孙博王妍
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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