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Method for extracting brain strengthening bioactive peptide from brain tissue of farming animals

A technology of animal brain and active peptide, which is applied in the direction of drug combination, pharmaceutical formula, peptide/protein composition, etc., can solve the problem of waste of animal brain resources, and achieve the effect of high molecular rate, easy storage and easy absorption

Inactive Publication Date: 2011-08-03
DANDONG SHUANGZENG FOOD DEV GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

cause waste of animal brain resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Obtaining animal brain tissue:

[0028] 1.1 Disinfection of animals

[0029] Clean and disinfect healthy animals with 50-200ppm / L chlorine dioxide disinfectant, and the temperature of the disinfectant should be controlled at 18°C-26°C.

[0030] 1.2 Animal stunning

[0031] Applied physical (such as mechanical electric shock) or chemical (inhalation of CO 2 ) method to put the animal in a coma for a short time before taking the brain.

[0032] 1.3 Vacuum extraction of animal brain tissue

[0033] Stainless steel tubular vacuum gun is used, the diameter of the vacuum gun barrel is 5-16mm, the length is 100-200mm, and the vacuum degree is controlled at 100-300pa. The stunned animal is pierced through the eye socket or nostril into the animal's ventricle. Under vacuum state, the animal brain tissue is taken out and sucked into the vacuum storage container, so as to maintain the activity and freshness of the animal brain tissue cells.

[0034] 1.4 Preservation of an...

Embodiment 2

[0054] Take 200 healthy pigs, prepare 100ppm / L chlorine dioxide disinfection solution, and wash and disinfect the pigs in batches at 22°C. The sterilized pigs are stunned by electric anesthesia. First, the eyes of the pigs are sterilized with ethanol, and then a vacuum gun is used to penetrate the eyes of the pigs into the brain ventricles of the pigs. Start the vacuum device. When the vacuum degree is 800pa, the pigs’ brain tissue Each pig brain can be extracted into the vacuum tank separately, and an average of 200g of pig brain can be extracted. This batch of 200 pig brains can extract 20kg of fresh pig brain tissue. After extraction, the pigs immediately enter the slaughtering and segmentation production line for processing.

[0055] Extract fresh 20kg pig brain tissue, cut it into a paste with a meat grinder, add 50kg of distilled water into a stainless steel 200L high-shear emulsifier, start the shearer and use steam to heat the shearer circulation heating device, so that...

Embodiment 3

[0057] The difference from Example 2 is: take 100 cows. The average amount of bovine brain extraction is 300g. After extraction, 30kg of fresh bovine brain tissue was quickly frozen and kept fresh in a quick-freezing room at -30°C to -35°C. When extraction is needed, slow-frozen 30kg of fresh-preserved quick-frozen bovine brain tissue in water at 20°C to remove the frozen layer, thawed in warm water at 30°C, and then processed the brain tissue: lipolysis, proteolysis, enzyme modification, ultramicro Membrane separation, low-temperature concentration, molecular embedding, and centrifugal spray drying to obtain 6 kg of bovine brain active peptide powder. After compounding, granulating, drying and pressing, each tablet is 300mg, and it is coated into 10 tablets per plate, and 20,000 brain-invigorating active peptide tablets can be made into finished products.

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Abstract

The invention relates to a method for extracting brain-nourishing bioactive peptide through adopting animal brain tissue, in particular to a method for extracting brain-nourishing bioactive peptide from the brain tissue of livestock. The method adopts the following steps of animal brain tissue obtaining, vacuum suction of animal brain tissue, directional biological enzymolysis, bioactive peptide separation of animal brain, low-temperature concentration, molecular embedding and spray drying so as to make brain-nourishing bioactive peptide finished product. The method has the advantage that: bioengineering technology is adopted to promote the development of livestock product processing industry towards a high-tech and high-added value industry. Moreover, the method provides a new opportunity to make animal brain resources which is regarded as having low biological value into brain-nourishing bioactive peptide with biological activity and meeting better the requirements of human physiological function for health-care nutrition under the action of biological enzyme engineering and ultramicromembrane separation engineering, etc.

Description

technical field [0001] The invention relates to a method for extracting brain-invigorating active peptides from animal brain tissues, in particular to a method for extracting brain-invigorating active peptides from animal brain tissues. Background technique [0002] my country has a large number of abundant animal brain tissue resources. Due to the low utilization rate of raw materials, the heads and hooves produced in the processing of animal products are treated as leftovers and by-products. Cause a waste of animal brain resources. In order to increase the added value in the processing of animal products, studies have shown that the head and midbrain tissue of animal product processing is rich in effective physiologically active substances such as protein, amino acid, cephalin, and vitamins. Using modern biotechnology for intensive processing, amino acids, small molecular peptides, cephalin and other multifunctional factors are extracted from animal brain tissue to form a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/00A61K38/17A61K35/30A61K9/20A61K9/48A61P25/00
Inventor 李长青宋兴华冷毅李小龙王红
Owner DANDONG SHUANGZENG FOOD DEV GROUP
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