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A composition comprising an extract of tiarell polyphylla and tiarellic acid isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity

A technology of composition and extract, which is applied in the field of xanthic acid composition, can solve the problems of no report or publication of xanthic acid's inhibitory effect, and achieve the effect of safe use

Inactive Publication Date: 2008-07-16
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there is no report or disclosure in any of the above-cited documents on the inhibitory effects of twig extract and xantholic acid isolated therefrom on inflammatory, allergic and asthmatic diseases, the disclosures of which are incorporated herein by reference

Method used

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  • A composition comprising an extract of tiarell polyphylla and tiarellic acid isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity
  • A composition comprising an extract of tiarell polyphylla and tiarellic acid isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity
  • A composition comprising an extract of tiarell polyphylla and tiarellic acid isolated therefrom having antiinflammatory, antiallergic and antiasthmatic activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 The preparation of the crude extract of Shuizhi

[0055] It was collected in Ulleungdo, South Korea in August 2003, and its voucher specimen (PEB 3068) was collected by the Korea Research Institute of Bioscience and Biotechnology (KRIBB) located in Daejeon, Korea. ) plant extract library preservation.

[0056] Cut 1.1kg of dried twigs into small pieces, mix with 5L of methanol, stir the mixture at room temperature for 24 hours, and extract three times with cold water. Filter the extract with filter paper to remove residue. The filtrate was collected, concentrated under reduced pressure using a rotary evaporator at 55-65° C., and dried with a freeze dryer to obtain 100.5 g of dried crude extract of Shuizhi chrysanthemum.

Embodiment 2

[0057] The preparation of embodiment 2 butanol soluble components

[0058] Add 1 L of distilled water to 100.5 g of the crude extract obtained in Example 1, place it in a separatory funnel, add 1 L of butanol, and shake vigorously to separate it into a butanol-soluble layer and a water-soluble layer.

[0059] The above-mentioned butanol-soluble layer was concentrated using a rotary evaporator, and dried with a freeze dryer to obtain a butanol-soluble extract. Finally, 80.0 g of butanol-soluble extract and water-soluble extract were obtained, which were used as samples in the following experiments.

Embodiment 3

[0060] Example 3 Preparation of xicholic acid from the extract of twig

[0061] 3.29 kg of dried Cl. japonica plants were extracted twice with methanol (10 L) at room temperature to obtain 352 g of extract. The extract was suspended in 1 L of water, and the phases were separated with an equal volume of n-hexane. Then 65.1 g of n-hexane soluble fractions were subjected to silica gel column chromatography (70-230 mesh, 8.5×65 cm), and were washed successively with n-hexane-ethyl acetate mixture (10-20% ethyl acetate, stepwise gradient) and chloroform - Elution with methanol mixture (0-100% methanol, step gradient) to obtain 9 fractions (Fr.1-Fr.9). 7.4 g of component 6 (chloroform-methanol 9 / 1-7 / 3, between v / v) was subjected to column chromatography using a normal phase silica gel column (silica gel, 230-400 mesh, 6.0×60 cm, chloroform-methanol mixture, 5-50% methanol, stepwise gradient) to obtain 400 mg xantholic acid. Using those methods reported before (Park et al., ArchPh...

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Abstract

The present invention relates to a composition having anti-inflammation, anti-allergic and anti-asthma effects, comprising the extract of Shuizhi and xantholic acid isolated therefrom. Extracts of twigs and twig acid isolated therefrom showed inhibitory effects on LTC4 release in vitro, as well as on IgE levels and cytokine (IL-4, IL-5, and IL-13) production in OVA-induced asthmatic mice , airway hyperresponsiveness and inhibition of leukocyte infiltration. Therefore, they are useful as therapeutic agents or functional health foods for the treatment and prevention of inflammatory, allergic and asthmatic diseases.

Description

technical field [0001] The present invention relates to a composition comprising Tiarella polyphylla extract and tiarellic acid isolated therefrom having anti-inflammatory, anti-allergic and anti-asthmatic effects. Background technique [0002] Asthma has been recognized as a complex syndrome arising in the airways manifested by various disturbances such as airflow obstruction, acute or chronic inflammation, airway hyperresponsiveness (AHR) and structural remodeling (Kumar R.K.Pharmacol. Ther., 91, pp 93-104, 2001). [0003] Allergic inflammation occurring in the airways has been reported to play a key role in the development of asthma, and the number of patients with allergic asthma has recently increased to about 10% of the world's population. According to reports, the number of patients in the United States has reached 17 million, and the market size of allergic asthma drugs in the United States has expanded to 640 billion US dollars so far. [0004] Asthma can be divid...

Claims

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Application Information

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IPC IPC(8): A61K36/185A23L1/29A23L33/00
CPCA23L29/035A61K9/08A61K9/20A61K9/48A61K31/56Y10S424/81A23V2200/304A23V2200/314
Inventor 李烔圭吴世湸安璄燮李重求李相龟金正姬金斗英全桂花权玉京金银娥崔顺子沈光海金美真朴普映闵炳善韩均熙朴惠英金健哲金柱宪金素英
Owner KOREA RES INST OF BIOSCI & BIOTECH
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