Cosmetic product clindamycinum high efficiency liquid chromatography detection method

Abstract

The invention provides an analysis method for detecting the clindamycin in cosmetic by use of high efficiency liquid chromatograph, and the invention is characterized in that octadecylsilane chemically bonded silica is adopted as the C18 column or the C8 column of filler, and an ultraviolet detector or a diode array detector are used to detect at the position of 190 to 380 nm; the mobile phase adopts ammonium dihydrogen phosphate solution-acetonitrile buffer solution or ammonium dihydrogen phosphate solution-methanol buffer solution; the sample is dissolved and extracted by dried methyl alcohol or acetonitrile, and, after extracted by ultrasound for 20 minutes, is centrifuged in 12000rpm / min for around 5 minutes. The clear liquid is the sample liquid, which is, if necessary, filtered by 0.45Mu meters filtering membrane for preparation; the qualitative analysis is conducted according to the sample retention time and the spectrogram, and the quantitative analysis is conducted according to the peak area. The invention has the advantages of high solution and sensitivity, and perfect reproducibility and selectivity as well as simple sample pre-treating method, thereby being applicable to microanalysis of clindamycin in cosmetic.

Description

Technical field [0001] The invention is a new technology for detecting clindamycin in cosmetics by using high performance liquid chromatography, and is especially suitable for qualitative and quantitative analysis of clindamycin, a trace antibiotic in cosmetics. Background technique [0002] Clindamycin is a macrolide antibacterial drug. As a broad-spectrum and highly effective antibacterial drug, it is widely used in medicines, feeds, and cosmetics. Clindamycin has a strong inhibitory effect on anaerobic bacteria; it also has a considerable inhibitory effect on sensitive Gram-positive bacteria. Due to its broad-spectrum and high-efficiency antibacterial effect, clindamycin is added to acne products by some cosmetic manufacturers to achieve the purpose of anti-inflammatory, anti-acne, mites and anti-acne. However, this drug also has many adverse reactions, such as: easy to cause pseudomembranous enteritis, easily lead to intestinal flora imbalance, have a neuromuscular blockade, ...

AI Technical Summary

Problems solved by technology

Capillary electrophoresis high-frequency conductometric method and linear sweep voltammetry have low sensitivity, but the steps are complicated, there are many influencing factors, and the reproducibility is poor

High-performance liquid chromatography is currently the most commonly used method for the detection of clindamycin, but it is only limited to the content detection of various clindamycin preparations, and the content of clindamycin in cosmetics, feed, and meat There are no relevant standards or methods for the detection. Because the drug has fewer components, the matrix is ​​simple, the interference factors are less, and the sample processing is simple, the loss of the target detection substance is small, so the clindamycin in the drug is easy to detect

Different from medicines, cosmetics have various types, complex matrices, large differences in the physical and chemical properties of each component, and many interfering factors. In particular, clindamycin is added to cosmetics as a prohibited ingredient in cosmetics, and its content is relatively low. It is difficult to accurately detect trace amounts of clindamycin in cosmetics, and the method of using high performance liquid chromatography to detect clindamycin in cosmetics has not been reported in the literature

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