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Bentonite, biological polysaccharide plural gel immobilized cell carrier and preparation method thereof

A technology for immobilizing cells and composite gels, applied in the biopolysaccharide composite gel immobilized cell carrier and its preparation, in the field of bentonite, can solve the problems of high curing temperature, poor mechanical strength, poor acid resistance, etc., and achieves low curing temperature, Good operability and high mechanical strength

Inactive Publication Date: 2008-04-09
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Immobilized cell carrier balls made of single carrageenan or carrageenan combined with glucomannan or konjac polysaccharide have high curing temperature, poor mechanical strength, and poor acid resistance, which limit their application in immobilized cells

Method used

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  • Bentonite, biological polysaccharide plural gel immobilized cell carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A bentonite and biological polysaccharide composite gel immobilized cell carrier is made of the following raw materials in parts by volume:

[0031] 40 parts of bentonite suspension aqueous solution;

[0032] 40 parts of complex polysaccharide aqueous solution;

[0033] 120 parts of potassium chloride aqueous solution;

[0034] The concentration of the bentonite suspended aqueous solution is 0.02 g / ml; the concentration of the complex polysaccharide aqueous solution is a mixed aqueous solution of 0.024 g / ml carrageenan and guar gum, wherein the weight ratio of carrageenan and guar gum It is 5: 0.8; the weight percentage concentration of described potassium chloride aqueous solution is 1.5%;

[0035] The complex polysaccharide aqueous solution is a mixed aqueous solution of carrageenan and guar gum maintained at 55° C. after the complex polysaccharide is dissolved in 80° C. water.

[0036] A preparation method of bentonite and biological polysaccharide composite gel i...

Embodiment 2

[0048]A bentonite and biological polysaccharide composite gel immobilized cell carrier is made of the following raw materials in parts by volume:

[0049] 60 parts of bentonite suspension aqueous solution;

[0050] 60 parts of complex polysaccharide aqueous solution;

[0051] 180 parts of potassium chloride aqueous solution;

[0052] The concentration of the bentonite suspended aqueous solution is 0.04 g / ml; the concentration of the complex polysaccharide aqueous solution is a mixed aqueous solution of 0.048 g / ml carrageenan and guar gum, wherein the weight ratio of carrageenan and guar gum Is 5: 1.2; The weight percent concentration of described potassium chloride aqueous solution is 2.5%;

[0053] The complex polysaccharide aqueous solution is a mixed aqueous solution of carrageenan and guar gum maintained at 65 °C after the complex polysaccharide is dissolved in water at 120 °C.

[0054] A preparation method of bentonite and biological polysaccharide composite gel immobi...

Embodiment 3

[0066] A bentonite and biological polysaccharide composite gel immobilized cell carrier is made of the following raw materials in parts by volume:

[0067] 50 parts of bentonite suspension aqueous solution;

[0068] 50 parts of complex polysaccharide aqueous solution;

[0069] 150 parts of potassium chloride aqueous solution;

[0070] The concentration of the bentonite suspended aqueous solution is 0.03 g / ml; the concentration of the complex polysaccharide aqueous solution is a mixed aqueous solution of 0.036 g / ml carrageenan and guar gum, wherein the weight ratio of carrageenan and guar gum It is 5: 1; The weight percent concentration of described potassium chloride aqueous solution is 2%;

[0071] The complex polysaccharide aqueous solution is a mixed aqueous solution of carrageenan and guar gum maintained at 60 °C after the complex polysaccharide is dissolved in water at 100 °C.

[0072] A preparation method of bentonite and biological polysaccharide composite gel immobi...

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Abstract

The invention relates to an immobilized cell carrier of bentonite and biological polysaccharide compound gel, which comprises the materials according to the following volume proportion: 40 to 60 proportions of bentonite suspension solution, 40 to 60 proportions of compound polysaccharide solution, 120 to 180 proportions of potassium chloride solution; concentration of the bentonite suspension solution is 0.02 to 0.04 g / ml; concentration of the compound polysaccharide solution is 0.024 to 0.048 g / ml which is a mixed solution of carrageenan and guar gum, wherein, weight ratio of the carrageenan and the guar gum is between 5 : 0.8 and 5:1.2; concentration of the potassium chloride solution is 1.5 to 2.5 percent. The invention adopts bentonite, biological polysaccharide compound as materials to prepare polymer sphere, which has the advantages of high mechanical strength, wide scope of pH value application, good heat stability, poisonless and good biocompatibility, and can be used for various fields such as foodstuff, biomedicine, bioengineering, etc., as an immobilized cell carrier.

Description

technical field [0001] The invention relates to a bentonite and biological polysaccharide composite gel immobilized cell carrier and a preparation method thereof. Background technique [0002] Immobilized cell carrier is a new type of functional polymer material developed in the past thirty years. Immobilized cell carrier spheres can bind biologically active substances such as plants, animals, and microbial cells through embedding and covalent bonds. Therefore, it has good operability and production performance, and can be applied in various fields. [0003] Scholars at home and abroad attach great importance to the study of immobilized cell carriers. Chinese patent CN1408859 discloses a penicillin acylase immobilized carrier and its preparation method, using a surfactant as a dispersant and a vinyl compound as a monomer, and adopting reversed-phase suspension technology for cross-linking polymerization to obtain penicillin acylation Enzyme immobilization carrier; Chinese...

Claims

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Application Information

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IPC IPC(8): C12N11/04C12N11/14
Inventor 张继王文强王云普张生堂姚健梁俊玉
Owner NORTHWEST NORMAL UNIVERSITY
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