Injection for face beauty and smoothing wrinkles and its preparation method

Abstract

The present invention relates to a new wrinkle free injection and the preparation method, which belongs to the tissue engineering field. The injection preparation comprises 105 to 108 newborn umbilical cord dermis fibroblast and the fiber anterior somatic or the mesenchymal cells, 1ml to 8ml limousine with concentration of 0.3 percent, the glucose injection with concentration of 5 percent. The preparation method comprises in vitro cultivation, purification and amplification of the newborn umbilical cord dermis fibroblast and the fiber anterior somatic or the mesenchymal cells. Then the cells are preserved in liquid nitrogen. The cells are massively proliferated during application. According to 105 to 108 newborn umbilical cord dermis fibroblast and the fiber anterior somatic or the mesenchymal cells, 1ml to 8ml limousine with concentration of 0.3 percent and the glucose injection with concentration of 5 percent are added. The present invention has the advantages of no damage to the body with material of newborn umbilical, high survival rate in cell, strong proliferation activity, short amplification time, no side effect, and no pain during injection, wrinkle free effect which is fast, profound, long and natural.

Description

technical field [0001] The invention relates to a cosmetic anti-wrinkle injection and a preparation method thereof, belonging to the field of tissue engineering. Background technique [0002] Over the years, many soft tissue filling materials have appeared in the field of cosmetic surgery. At present, the most commonly used filling materials at home and abroad are as follows: 20 years ago, the first filling material approved for human body wrinkle removal and scar treatment was bovine collagen. protein. Clinical trial was carried out in 1977, and it was approved by FDA in 1981. These bovine collagens generally stay in the tissue for 6 to 9 months, so the maintenance time is relatively short. In addition, because they are foreign proteins, they often present local allergic reactions and complications, such as transient reactions with bruises, reactive herpes, asthenia, etc. Bacterial abscess, etc., may cause skin necrosis and scar hyperplasia in severe cases. The second ty...

AI Technical Summary

Problems solved by technology

Its disadvantages are: to take autologous skin tissue cells, the patient has pain, and new scars are generated. Since they are adult fibroblasts, the number of divisions is small, the reproduction speed is slow, and the expansion time is long. Repeated injections are required. After the reaction is obvious, redness, swelling, edema, bruising, local inflammation, obvious pain, slow and unstable effect often appear, and it cannot be used for the elderly over 55 years old

The fourth commonly used is botulinum toxin injection, its fatal disadvantage is that it causes muscle paralysis, which makes people's facial expressions very unnatural

Other injections such as polymethyl methacrylate, hyaluronic acid preparations, and expanded polytetrafluoroethylene are used for wrinkle removal, but they are difficult to popularize because of many reactions and unsatisfactory effects.