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Method for preparing secoisolariciresinol diglucoside

A technology of secoisolarix and glucoside, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2008-02-06
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the prior art, NaOH is the main choice for catalytic bases. Although it is cheap, easy to use, and has obvious effects, it introduces a large amount of NaOH into the product at the same time. +

Method used

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  • Method for preparing secoisolariciresinol diglucoside

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Wet 100kg of defatted linseed cake with water, add yeast, ferment at 25±1°C for 48h; add 800L of ethanol solution with a volume concentration of 60%, extract at 60°C for 6h, extract twice in the same way, and wash with extraction solvent Filter cake 1 time; reclaim the solvent, dry to obtain 8.0kg of a product with a mass percentage of SDG of 9.5%;

[0066] The comparison group was extracted and dried by the same method as above, but no microbial fermentation treatment was carried out before extraction, and finally 7.2 kg of a product with a mass percentage of SDG of 5.3% was obtained.

Embodiment 2

[0068] Wet 100kg of defatted linseed cake with water, insert Bacillus, ferment at 37±1°C for 24h; add 800L of ethanol solution with a volume concentration of 70%, extract at 60°C for 6h, extract twice in the same way, and wash with extraction solvent Filter cake once; recover the solvent, and dry to obtain 8.8 kg of a product with a mass percentage of SDG of 8.9%.

[0069] The comparison group was extracted and dried by the same method as above, but no microbial fermentation treatment was carried out before extraction, and finally 8.0 kg of a product with a mass percentage of SDG of 6.0% was obtained.

Embodiment 3

[0071] Wet 100kg of defatted linseed cake with water, add mold, ferment at 28±1°C for 48h; add 800L of ethanol solution with a volume concentration of 70%, extract at 60°C for 6h, extract twice in the same way, wash and filter with extraction solvent Cake 1 time; recover the solvent and dry to obtain 10.5 kg of a product with a mass percentage of SDG of 9.8%.

[0072] The comparison group was extracted and dried by the same method as above, but no microbial fermentation treatment was carried out before extraction, and finally 7.6 kg of a product with a mass percentage of SDG of 5.5% was obtained.

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Abstract

The present invention provides an extracting and purifying method for improving the yield of SDG, and creates a SDG sequential extracting and purifying scheme based on the method. The technical route of the present invention is that operations of microbial fermentation, enzymatic degradation, and non-salt process are integrated in the SDG extracting and purifying technology process based on the prior art, thus greatly improving the efficiency of SDG extracting and purifying and lowering the manufacturing cost. The present invention also creates a SDG graded purifying process to prepare products, whose purities are respectively 8 to 10 percent, 20 to 40 percent, 50 to 60 percent, as well as 90 percent. The front two products need not column chromatographic separation, and the process operation is simple and safe, thus the present invention is economical and practical, and is suitable to the industrial production; the present invention can satisfy the needs of functional food ingredient and health food; later two products have mature technology, stable technology process, and high product purity, thus being able to satisfy the needs of pharmacological study and drug development.

Description

technical field [0001] The present invention relates to a method for extracting and purifying secoisolaricoresinol diglucoside (SDG) from linseed husk or linseed meal after oil extraction, in particular to the method of microbial fermentation and enzymatic hydrolysis in the process application. The present invention also relates to a method of fractional purification to obtain a desired concentration of SDG product. Background technique [0002] Secoisolariciresinol Diglucoside (SDG) is a kind of lignan, which is a kind of natural component formed by bimolecular polymerization of phenylpropanoid, and is a kind of natural phytoestrogens. It has anti-tumor effect, estrogen and anti-estrogen effect, anti-cancer, anti-arteriosclerosis, prevention of diabetes, inhibition of aromatase activity, inhibition of DNA and RNA synthesis, anti-virus, anti-fungal and other effects. It is widely used in the fields of food, clinical medicine and cosmetics. With the improvement of human li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C12R1/07C12R1/66C12R1/85
Inventor 吴文忠张春枝徐维锋刘明李代王素馨刘毅王涛苟东凯
Owner INNOBIO CORP LTD
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