Botryosphaeria rhodina polypeptide

A mature polypeptide and polynucleotide technology, applied to DNA/RNA fragments, cells modified by introducing foreign genetic material, enzymes, etc.

Inactive Publication Date: 2007-10-10
NOVOZYMES AS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In general, EST methods have two disadvantages with regard to the identification of secreted proteins: 1) Depending on the induction conditions used for the sequenced cDNA library, there is only a small amount of cDNA, usually 0.5%-15% or even 1-5% 2) All clones come from the cDNA pool (pool), the cDNA pool is obtained from these mRNAs that exist in proportion to each specific gene in the organism

Method used

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  • Botryosphaeria rhodina polypeptide

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preparation example Construction

[0286] Compositions comprising enzyme polypeptides and methods for their preparation

[0287] The invention provides compositions comprising a polypeptide of the invention and an excipient and methods of preparing such compositions comprising admixing a polypeptide of the invention and an excipient. In a specific embodiment, the polypeptide of the invention is the main (polypeptide) component of the composition, eg a one-component composition. Here, an excipient should be understood as any auxiliary agent or compound used to formulate the composition, including solvents, carriers, stabilizers and the like.

[0288] The composition may also contain one or more other enzymes such as aminopeptidase, amylase, carbohydrase, carboxypeptidase, catalase, cellulase, chitinase, cutinase, cyclodextrin sugar Base transferase, deoxyribonuclease, esterase, α-galactosidase, β-galactosidase, glucoamylase, α-glucosidase, β-glucosidase, haloperoxidase ), invertase, laccase, lipase, mannosidas...

Embodiment 1

[0341] Example 1 Identification of functional polypeptides secreted by Botryosphaeria rhodina CBS 274.96

[0342] Enzyme fingerprint analysis of culture medium

[0343] Enzyme activity profiles were obtained by assaying the culture broths with various enzyme assays. 96-well microtiter (MT) plates with substrate were prepared and stored at 10°C until use. Two different pH variations were prepared: pH3 and pH7. The following substrates were used: 0.05% AZCL (Mazurine staining and cross-linking substrate, Megazyme) - amylose, arabinan, β-glucan (barley), casein, collagen, curdlan, dextran Sugars, galactan (potato), galactomannan (carob) (carob), He-cellulose, pullulan (pullulan), xylan (oat) and xyloglucan (AZCL-casein Egg whites cannot be used at pH 3, so no AZCL-casein was added to these plates).

[0344] Preparation of pH3 substrate:

[0345] 0.1 g of each AZCL substrate was dissolved in 100 ml of 0.2M succinic acid pH 3 + 10 microliters of Triton X-100 (0.01%) to give a ...

Embodiment 2

[0402] Example 2 Determination of Enzyme Activity by Homology

[0403] The function of a gene or encoded polypeptide can be predicted by comparison with genes or polypeptides of known function. Analyzed a DNA Group and B 多肽 Similarity between group sequences and sequences from public and internal databases to determine a DNA Group and B 多肽 Functionality of group sequences. Sequence comparisons were performed with the program BLASTX2.0a19MP-WashU [1998-7-14]. by adding A DNA Group and B 多肽 Careful manual alignment of the group sequence with its closest sequence of known function provides the possibility to predict the function of these genes and encoded polypeptides. Even in cases where overall amino acid identity is below 40%, making reliable predictions may be difficult, by careful analysis and interpretation of amino acid residues in important regions of the catalytic site and / or polypeptide sequence, A DNA Group and B 多肽 Functions of group sequences are also possib...

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PUM

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Abstract

The invention relates to functional polypeptides secreted from Botryospaeria rhodina CBS 247.96.

Description

field of invention [0001] The present invention relates to a functional polypeptide encoded by the polynucleotide contained in the mRNA of Diplodiagossypina (Diplodiagossypina) deposited under the deposit number CBS 274.96, synonymously named Botryospaeria rhodina. The invention also relates to polynucleotides encoding these polypeptides or facilitating their expression, or constructs of these polynucleotides, and methods of making the polypeptides. The present invention also relates to compositions comprising the polypeptide and uses of the polypeptide. Background technique [0002] Botryosphaeria rhodina (Berk. & M.A. Curtis) Arx, (synonym Diplodiagossypina Cooke). There are very few published reports on the characterization of this organism; Selbmann et al. reported the exopolysaccharide production of Botryosphaeria rhodina in 2003 (Selbmann L, Stingele F and Petruccioli M (2003). Exopolysaccharide production by filamentous fungi: the example of Botryosphaeria rhodina. ...

Claims

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Application Information

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IPC IPC(8): C12N15/52C12N9/42C12N9/00C12N5/10
Inventor 柯克·M·施诺尔莱纳·兰格珀尼尔·U·博尔韦格
Owner NOVOZYMES AS
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