Verdigris pseudomonads testing fluid and simple testing bottle
A technology of Pseudomonas aeruginosa and test solution, which is applied in the determination/inspection of microorganisms, biochemical instruments, biochemical equipment and methods, etc., and can solve the problems of high quality requirements for the test environment and operators, misjudgment of test results, and operational problems. cumbersome procedures and other issues, to achieve accurate and reliable test results, avoid contamination and interference, and save construction investment
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Embodiment 1
[0022] Embodiment 1: Referring to Fig. 1, among the figure 1 is an aluminum skin cover, 2 is a rubber stopper, 3 is a glass bottle cavity with equal cross-section, and 4 is a test solution for Pseudomonas aeruginosa. h 1 is the height of Pseudomonas aeruginosa test solution, H 2 Empty height for the equal section of the test bottle. In the present embodiment, the test bottle is a colorless transparent oral liquid bottle of 15mL, and the height H of the test solution is 1 is 6cm, and the height of the space is H 2 It is 4cm.
[0023] The formula of the above test solution is: 0.02% cetyltrimethylammonium bromide, 0.2% sodium chloride, 0.5% peptone, 0.1% beef extract. In a colorless transparent oral liquid bottle, it can be sterilized by high pressure for 15 minutes.
[0024] When in use, the extinction dilution method is used, that is, the water sample to be determined is injected into the test bottle step by step with a sterile syringe (solid samples must be dissolved or ...
Embodiment 2
[0025] Embodiment two: the present embodiment is basically the same as embodiment one, and the difference is that the test bottle is a colorless transparent vial of 8mL, and the height H of the test solution is 1 is 4.5cm, and the height of the space is H 2 It is 3.5cm.
[0026] The formula of the above test solution is: 0.15% cetyltrimethylammonium bromide, 1.0% chloride, 2.0% peptone, 0.5% beef extract, and the balance is distilled water. After the above-mentioned test solutions are prepared, pack 4.5 mL per bottle into 8 mL colorless transparent vials, and sterilize under high pressure for 15 minutes.
[0027] When using, use a sterile syringe to draw 0.5mL water quality sample, inject it into the test bottle, shake it well and place it in a 37°C drying oven or water bath for 24 hours, and judge the negative or positive reaction according to the change of the liquid in the bottle, which is qualitative. Analysis; make 2 or 3 sets of parallel samples according to this metho...
Embodiment 3
[0028] Embodiment three: the present embodiment is basically the same as embodiment one, and the difference is that the test bottle is a colorless transparent round-bottomed glass bottle of 10mL, and the height H of the test solution is 1 is 3.0cm, and the height of the space is H 2 It is 0.9cm.
[0029] The formula of the above test solution is: cetyltrimethylammonium bromide 0.04, sodium chloride 0.2, peptone 0.5%, beef extract 0.1%, distilled water 96.4-99.6%, and the balance is distilled water. After the above test solution is prepared, pack 4.5 ml of each bottle in a 10 mL test bottle and autoclave for 15 minutes.
[0030] Using method is the same as embodiment one.
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