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Process for producing triglyceride containing three residues of one highly unsaturated fatty acid and use thereof

A technology of unsaturated fatty acids and triglycerides, which is applied in the efficient and stable production of the above-mentioned triglycerides, triglycerides and their representative applications, which can solve the problem of high calories and achieve the effect of low calories and increased added value

Inactive Publication Date: 2007-07-25
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In addition, when humans and animals ingest lipids containing PUFAs in anticipation of specific PUFA physiological actions, they are often ingested in the form of triglycerides from the viewpoint of physical properties such as stability and low irritation to organisms, but Concerned about being high in calories as it is a lipid

Method used

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  • Process for producing triglyceride containing three residues of one highly unsaturated fatty acid and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1. Obtained by mutation treatment of Mortierella alpina (Mortierella alpina) SAM2268 A high-yield strain of triarachidonoylglycerol

[0080] Mortierella alpina ( Mortierella alpina ) SAM2268 (FERM P-17762) was inoculated into Czapek agar medium (0.2% NaNO 3 , 0.1% K 2 HPO 4 , 0.05% MgSO 4 ·7H 2 O, 0.05% KCl, 0.01% FeSO 4 ·7H 2 O, 3% sucrose, 2% agar, pH 6.0) in a 300mL large slant culture flask, cultured at 28°C for 2 weeks.

[0081] After the incubation, add 50 mL of sterilized water, shake and mix, filter with 4 layers of gauze, centrifuge at 8,000×g for 10 minutes, and suspend in 50 mM Tris buffer solution (pH 7.5) to prepare a spore suspension. in 1×10 6 0.5 mL of 0.5% NTG (N-methyl-N'-nitro-N-nitrosoguanidine) solution was added to 1.5 mL of spore suspension, and mutation treatment was performed at 28°C for 15 minutes. Add 10%Na 2 S 2 o 3 3 mL, centrifuged at 5,500×g for 10 minutes, washed with sterilized water to obtain NTG-treated spor...

Embodiment 2

[0084] Example 2. Comparison of production capacity of triarachidonoylglycerol

[0085] In 10 mL of liquid medium (glucose 2%, yeast extract 1%, palmitoleic acid 1% or nothing) contained in a 50 mL Erlenmeyer flask, inoculate an inoculation loop of the following 3 strains of Mortierella alpina (Mortierella alpina ), cultured at 28° C. with shaking at 120 rpm for 7 days.

[0086] (1) Mortierella alpine#1 (mutant strain obtained in Example 1)

[0087] (2)Mortierella alpina SAM 2268

[0088] (3)Mortierella alpina IFO 8568

[0089] After the cultivation, the bacterial cells were collected by filtration and dried. After drying a part of the grown bacterial cells, triglycerides were extracted with hexane according to a conventional method, and the hexane was distilled off to obtain triglycerides. The obtained triglyceride was subjected to high performance liquid chromatography, and the ratio of triarachidonoylglycerol (tri-AA) was determined by analyzing the molecular specie...

Embodiment 3

[0093] Example 3. Time-dependent changes in the production of triarachidonoylglycerol

[0094] Inoculate a loop of Mortierella alpina #1 into 10 mL of liquid medium (glucose 2%, yeast extract 1%, palmitoleic acid 1% or none) in a 50 mL Erlenmeyer flask, at 28°C Under 120rpm shaking culture for 6, 8, 10 days. After the cultivation, the bacterial cells were collected by filtration, and the ratio of triarachidonoylglycerol (tri-AA) and the ratio of arachidonic acid in total fatty acids were determined in the same manner as in Example 2. The results are shown in Table 2.

[0095] Table 2 Time-dependent changes of triarachidonoylglycerol (tri-AA) production in #1 strain

[0096] Training days (days)

[0097] Regarding the ratio of tri-AA, it was 30.1% on the 6th day, 45.9% on the 8th day, and 62.5% on the 10th day.

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Abstract

A process for producing a fat in which a triglyceride formed by the bonding of molecules of one highly unsaturated fatty acid respectively to the three hydroxy groups of glycerol accounts for 20 wt.% or more of all triglycerides, characterized by incubating a microorganism capable of yielding the fat and collecting the fat according to need; and a fat obtained by the process.

Description

technical field [0001] The present invention relates to a method for producing triglycerides composed of three identical polyunsaturated fatty acid residues and its use, and more particularly to a method for efficiently and stably producing the above-mentioned triglycerides and triglycerides obtained by the production method and its representative applications. Background technique [0002] The polyunsaturated fatty acid (PUFA) mentioned here means the fatty acid which has 18 or more carbon atoms and has 2 or more double bonds. PUFAs have various unique physiological activities, and thus are used in various foods and animal feeds to improve their functionality. The main PUFAs are: linoleic acid (LA), α-linolenic acid (ALA), γ-linolenic acid (GLA), double homo-γ-linolenic acid (DGLA), Mead acid (MA), arachidonic acid (AA ), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), etc. Sometimes it is used in the form of free fatty acids and phospholipids, but it is mainly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C11C3/00C12N1/14C12P7/6472
CPCA23K1/164C11B1/10C12P7/6472A23D9/00A23L1/3006A23L2/52A23K20/158A23L33/115C12P7/64C11C3/00C12N1/14
Inventor 河岛洋樱谷英治小川顺清水昌
Owner SUNTORY HLDG LTD
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