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Mixture with dust mite for treating allergy

A technology of dust mite and dust mite, which is applied in the field of pharmaceutical composition for the prevention and treatment of allergic diseases and its preparation, which can solve the problems of insufficient dosage, decreased potency, dust mite allergy, etc., and achieves wide application range and wide application Effects of outlook and clinical value

Active Publication Date: 2009-09-09
ZHEJIANG WOLWO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] 2. Dust mite allergens
There are two problems with mixed vaccines: one is that excessive dilution of multiple allergens may lead to insufficient dosage of each allergen; the other is that because one allergen contains active enzymes, each allergen cannot The potency will drop rapidly
However, for related allergens, they have the same antigenic determinant, which will cause cross-reaction, such as house dust mite and dust mite, these related allergens, whether the clinical application of monovalent vaccine or mixed vaccine has no essence gender difference

Method used

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  • Mixture with dust mite for treating allergy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Example 1 Preparation of Dermatophagoides farinae allergen leachate

[0071] 1) Degreasing and drying

[0072] Use flour or animal feed as the medium (flour: yeast powder = 10:1) to cultivate the standard species of D. farinae, and sieve (use a 120-mesh sieve to remove mite bodies of D. farinae) from the D. farinae metabolic medium continuously. Soak and degrease with acetone for 3 times, each time for 4 hours, until the acetone after degreasing is colorless, dry the solid after degreasing naturally until there is no smell of acetone, and then weigh it. The laboratory operation should be completed in a fume hood, and the pilot test uses a vacuum concentration extraction tank.

[0073] 2) extract

[0074] In order to ensure that the active ingredients are not inactivated, the extraction should be completed at 4°C. The detailed steps are: the degreasing D. farinae metabolic medium obtained in step 1) and physiological saline are extracted in a ratio of 1:10 (W / V) (that...

Embodiment 2

[0079] Example 2 Preparation of House dust mite allergen leach solution

[0080] 1) Cleaning, grinding, degreasing, drying

[0081] Cultivate house dust mites in a culture medium (2 parts of laboratory animal feed, 2 parts of dry yeast, 1 part of dry fish meal, and the humidity of the medium is 16%) so that the density reaches 300-500 per gram. Use saturated NaCl solution to suspend and separate, and collect dust mite mite bodies.

[0082] The obtained dust mite mite bodies were suspended and washed with physiological saline, dried and stored at -20°C for later use. Weigh the worm body, grind the worm body with liquid nitrogen, soak and degrease it with acetone for 3 times continuously, 4 hours each time, until the acetone after degreasing is colorless, dry the degreased solid naturally until there is no smell of acetone, and then weigh it. Heavy.

[0083] 2) extract

[0084] Soak and extract the dust mite body and normal saline at a ratio of 1:25 (W / V) (i.e. extract with ...

Embodiment 3

[0089] The preparation of embodiment 3 dosage form

[0090] 1) Dilute the extracts obtained in Examples 1 and 2 with physiological saline and mix them at a ratio of 50:1 (volume ratio), and then add an equal volume of glycerin to make a mixed allergen preparation. According to the concentration required for treatment, dilute it into preparations with multiple concentrations ranging from 1:100 (V / V) to 1:100000000 (V / V); sub-package and potting; inactivate at 56°C for about 1 hour to sterilize, Immediately available sublingual buccal preparations;

[0091] 2) Take the above stock solution to prepare injections, tablets or capsules according to conventional preparation methods.

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Abstract

The invention provides a pharmaceutical composition for treating allergic diseases, which comprises a dust mite allergen, a dust mite allergen and a pharmaceutically acceptable carrier. The invention also relates to a preparation method of the pharmaceutical composition. The composition of the invention can be used for the treatment of allergic asthma, allergic rhinitis, allergic dermatitis, chronic urticaria and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology medicines, and in particular relates to a pharmaceutical composition for preventing and treating allergic diseases through a specific immunotherapy method and a preparation method thereof. Background technique [0002] 1. The relationship between dust mites and allergic asthma and allergic rhinitis [0003] Asthma is one of the most common diseases in the world today, and it is also a recognized medical problem in the world. It is listed by the World Health Organization (WHO) as one of the four major persistent diseases. WHO defines asthma as a lifelong disease. According to the latest estimates from World Asthma Day in 2003, 1 in 20 people in the world currently suffers from asthma. Preliminary findings from the Global Burden of Asthma report show that asthma is currently one of the most common chronic diseases in the world, affecting approximately 300 million people. According to the Global Asthma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/35A61K9/08A61K9/10A61K9/12A61K9/48A61K9/70A61P37/08
Inventor 胡赓熙
Owner ZHEJIANG WOLWO BIOTECH
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