Colonization probiotic preparation and application and medicines thereof

A technology of probiotic preparations and probiotics, applied in the field of colonization probiotic preparations and their applications and medicines, can solve the problems of difficult adhesion adhesion rate and difficulty in fully exerting the function of probiotics, achieve high adhesion rate, increase adhesion rate, broad The effect of applying the foreground

Active Publication Date: 2017-06-27
邵萍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, the existing probiotic preparations that act on the respiratory mucosa can be taken away from the respiratory tract by the mucus blanket driven by cilia in just over 10 minutes under the transport of respiratory mucus. Therefore, the existing probiotic preparations that directly act on the respiratory mucosa have difficulties Adhesion, that is, the problem of low adhesion rate, which makes it difficult for the corresponding probiotics to fully exert their functions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] The colonization probiotic preparation provided in this embodiment is used to directly act on the mucous membrane of the respiratory tract, including surfactants and probiotics. Wherein, the probiotic is Lactobacillus salivarius, and the surfactant is lichenysin, wherein the concentration of Lactobacillus salivarius is 1×10 8 CFU / ml, lichenin content is 5mg / L, surface tension is 55mN / m, NaCl content is 9g / kg, pH is 5.5. The colonization probiotic preparation is an aqueous suspension.

[0077] 1.1 The preparation method of the colonization probiotic preparation provided by this embodiment:

[0078] 1.1.1 Lactobacillus salivarius strain (Lactobacillus salivarius, ATCC 11741) was selected for cultivation. Culture conditions: 28°C, 5% inoculum, MRS as the medium, anaerobic culture, the initial pH of the MRS medium is 5.5, and culture for 15 hours.

[0079] 1.1.2 After the cultivation, centrifuge at 4500rpm for 10min to collect Lactobacillus salivarius cells.

[0080] 1....

Embodiment 2

[0114] The colonization probiotic preparation provided in this embodiment is used to directly act on the mucous membrane of the respiratory tract, including surfactants and probiotics. Wherein, probiotic is Lactobacillus casei, surfactant is surfactant (surfactin), the surface tension of this colonization probiotic preparation is 55mN / m, and pH is 6, and NaCl content is 9g / L, and surfactant (surfactin) The content of probiotics is 14.5mg / L, and the content of probiotics is 1×10 8 CFU / ml.

[0115] 2.1 The preparation method of the colonization probiotic preparation provided in this example is as follows:

[0116] 2.1.1 Lactobacillus casei (Lactobacillus casei, ATCC 393) was selected. Inoculate the fresh bacterial solution cultivated overnight and inoculate it into the MRS medium, the inoculum amount is 1%, the cultivation temperature is 28°C, and the anaerobic cultivation is left in the incubator for about 15 hours. After the cultivation, centrifuge at 4500rpm for 10min to c...

Embodiment 3

[0128] The colonization probiotic preparation provided in this embodiment is used to directly act on the mucous membrane of the respiratory tract, including surfactants and probiotics. Wherein, the probiotic is inactivated Lactobacillus casei, and the surfactant is surfactant. The surface tension of the probiotic preparation for colonization is 40mN / m, the pH is 5.5, the NaCl content is 9g / L, and the surfactant content is 16mg / L. L, the content of inactivated probiotics is 1×10 8 pieces / ml.

[0129] 3.1 The preparation method of the colonization probiotic preparation provided in this example:

[0130] The preparation method of the colonization probiotic preparation provided in this example is basically the same as step 2.1 in Example 2, except that in this example, after collecting Lactobacillus casei cells, heat inactivation at 80°C for 20 minutes, and then the inactivated Lactobacillus casei cells and sterile culture supernatant were used to prepare probiotics for coloniza...

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Abstract

The invention relates to the technical field of biology and discloses a colonization probiotic preparation and application and medicines thereof. The colonization probiotic preparation is used for directly acting on the respiratory mucosa and comprises a surfactant and probiotics. By the surfactant, modification of a sol layer and a gel layer of respiratory tract mucus is realized, probability and speed in contact of the probiotics with respiratory mucosa cells are increased, cell surface hydrophobicity of the probiotics is changed, an adhesion rate of the probiotics and respiratory mucosa epithelial cells is increased, and accordingly the technical bottleneck of difficulty in colonization of the probiotics to the respiratory mucosa under expelling action of a mucociliary transmission system is solved. The colonization probiotic preparation can be applied to fields of preparation of medicines for treatment of rhinitis, treatment of asthma, treatment of allergic dermatitis or treatment of cranial nerve diseases, anti-nasopharynx-cancer, anti-lung-cancer or anti-cold medicines or medicines for prevention or treatment of respiratory infectious diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a colonization probiotic preparation and its application and medicine. Background technique [0002] The respiratory tract of terrestrial vertebrates such as humans, livestock, and poultry circulates air containing microorganisms for a long time. The mucous membranes that line the inner surface of the respiratory tract constantly filter microorganisms in the air. Therefore, the respiratory tract of terrestrial animals inevitably has a wide variety of microorganisms. These microorganisms influence each other and also interact with the respiratory mucosa to form a sustainable microecology. [0003] Due to the connectivity of the respiratory tract, the microbial populations distributed on the respiratory tract of the same individual are basically the same. However, due to the mucociliary transport system (Mucociliary Transport System, MCT) of the respiratory tract, the number of micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/744A61K35/747A61K9/00A61K47/28A61K47/42A61K47/26A61P35/00A61P25/00A61P11/00A61P11/02A61P11/06A61P17/00A61P37/08
CPCA61K9/0082A61K35/744A61K35/747A61K47/26A61K47/28A61K47/42A61K2300/00
Inventor 杜斌
Owner 邵萍
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