Green trichodermin and its use in production of Pu'er tea
A technology of Trichoderma viride and Pu-erh tea is applied in the biological field to achieve the effects of stabilizing and improving quality, shortening processing time and improving quality
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Embodiment 1
[0012] Embodiment 1: Purification of PTri050102 bacterial classification
[0013] From the collected Pu'er tea samples, Trichoderma viride strains were obtained by separation on a known plate. Trichoderma viride purification medium is: 200g potato, 20g glucose, 20g agar, 1000ml distilled water, natural pH. Peel the potatoes, cut them into pieces, cook them for half an hour, then filter them with gauze, add agar and sugar, melt them and add water to 1000ml. Sterilize at 121°C for 20 minutes.
[0014] Purification method:
[0015] (1) Melt the known potato dextrose agar medium, cool it to 45°C, pour it into a sterile petri dish, about 15-20 milliliters per dish, shake slightly, let it stand, and make it into a flat plate for later use.
[0016] (2) Take 2g of the tea sample to be separated, add it to a mortar with 98ml of sterile water beside the flame and grind it, and what is obtained at this time is 10 -2 bacterial suspension.
[0017] (3) Dilution: Dilute the bacterial ...
Embodiment 2
[0021] Embodiment 2: the production application of Trichoderma viride
[0022] The maocha is moist-heated according to the traditional method, and then 0.1-0.8% by weight of the PTri050102 strain is added to the maocha to ferment it. When the pile temperature rises to 30°C, the PTri050102 strain begins to metabolize, and with As the pile temperature rises further, a large amount of enzymes are secreted. These enzymes oxidize and hydrolyze the ingredients contained in tea leaves. Under the action of protease, the non-digestible macromolecular protein is degraded into peptone, polypeptide and various amino acids. Under the action of glucoamylase and cellulase, the macromolecular insoluble substances of the fermentation substrate are converted into small molecular substances soluble in water. The Pu-erh tea produced after inoculation has a unique aroma, mellow taste and sweet aftertaste. Tan oily quality characteristics.
[0023] The quality of Pu'er tea produced after being i...
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