Netted tissue-engineering stand

A reticular tissue and engineering technology, used in tissue culture, tissue cell/virus culture devices, biochemical instruments, etc., can solve the problems of expensive medical polymer materials and tissue breakage, and achieve easy availability and good mechanical properties. Excellent performance, mechanical and biological properties

Active Publication Date: 2008-07-23
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, medical polymer materials are expensive and their degradation products are destructive to tissues

Method used

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  • Netted tissue-engineering stand
  • Netted tissue-engineering stand
  • Netted tissue-engineering stand

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weaving the thick silk into a mesh structure support (see figure 1 a, b, c, figure 2 ), and then with 0.2% Na 2 CO 3 The solution was boiled for 30, 60, 90, 120, 180 minutes. Under the electron microscope, it is found that the sericin protein on the surface of silk can be removed by cooking for more than 60 minutes (see image 3 b). The mechanical properties of the scaffolds boiled for 120 minutes did not change significantly. The bone marrow mesenchymal stem was planted on the silk support after removing the sericin, and electron microscope observation after culturing for 7 days showed that the cells could grow well on the support (see image 3 c).

Embodiment 2

[0037] The scaffold obtained by boiling for 90 minutes in Example 1 was seeded with mesenchymal stem cells, and the composite scaffold obtained in Example 2 was seeded with fibroblasts. After static or dynamic culture in vitro for 2-8 weeks, it can be found that the cells secrete extracellular matrix components and form sheets. Tissue engineered soft tissue (see Figure 4 ), which can be rolled or folded thick to form strip-shaped tissue engineered tendons and ligaments (see Figure 5 )

Embodiment 3

[0038] Example 3: Fill the mesh of the stent obtained by boiling for 90 minutes in Example 1 with collagen glue, and then freeze-dry it with a lyophilizer to obtain silk as a frame, and the collagen sea surface is filled with both good mechanics and sufficient Composite scaffolds on the cell attachment surface (see Figure 6 ).

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Abstract

A reticular scaffold for tissue engineering with high mechanical property and the space enough to hold cell tissue is prepared from silkworm silk without sericin through braiding. The collagen, hyaluronic acid, chitosan, chondrine sulfate, medical polyester, etc can be attached to it.

Description

(1) Technical field [0001] The invention relates to a mesh support for tissue engineering, in particular to a mesh support for tissue repair or reinforcement in vivo and tissue construction in vitro. (2) Background technology [0002] Connective tissue injuries such as tendons and ligaments in shoulders, knees, elbows and other joints are increasing (accounting for 50% of sports injuries). The number of tendon injuries in the hands is increasing by 5% every year due to lifestyle changes, such as typing on a keyboard and sending text messages on a mobile phone. Statistics show that there are at least tens of millions of tendon injuries per 200 million people a year. In addition, as the population age increases, more and more patients suffer from loose or defective soft tissues such as the abdominal wall and basin. [0003] At present, the injuries of tendons and ligaments as well as the relaxation of abdominal wall and basin tissues are mainly repaired and strengthened by a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/14A61L33/12A61L27/24A61F2/00A61B17/00C12M3/00C12N5/00
Inventor 欧阳宏伟邹晓晖王琳琳
Owner ZHEJIANG UNIV
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