The invention discloses a combination formula for regulating surface mucus layer barriers of a digestive duct and a respiratory tract and belongs to the field of medicines. The combination formula comprises Wolfiporia extensa, threonine, serine, cysteine, cysteine and proline, wherein the Wolfiporia extensa has the effect of enhancing blood supply and oxygen supply; threonine, serine, cysteine and proline are main constituents of mucoproteins. The combination formula provides nutrition, oxygen and raw materials for generation, regeneration, repair and mucoprotein of mucous glands and mucilage cells such as goblet cells and the like of the digestive duct and the respiratory tract; the combination formula is an effective combination formula for constructing normal surface mucus layer barriers of the digestive duct and the respiratory tract of a human body, preventing antigens from getting close to, being contacted with, adhering to and entering the digestive duct and respiratory tract tissue, blocking abnormal immune response, terminating inflammation, repairing the digestive duct and respiratory tract tissue and preventing and treating diseases of a digestive system and a respiratory system.