Cosmetic composition comprising white rose flower extract for skin whitening and improving skin wrinkle
a technology of cosmetic composition and white rose extract, which is applied in the field of functional cosmetic composition, can solve the problems of skin elasticity loss and wrinkle generation, deterioration of skin barrier to the outside, and generation of wrinkles, etc., and achieve the effects of enhancing skin elasticity, suppressing melanin production, and whitening
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example 1
Preparation of White Rose Petal
[0063]The white rose petal used in the present invention was collected at Jincheon, Chungbuk, Korea in May, 2010 and then completely dried under the sun and used. The completely dried petal was grinded and powdered by using a rotor speed mill (Laval Lab Inc., Laval, Que), sterilized by using a 70% ethanol spray, dried at 80° C., and then stored at 4° C. before usage.
example 2
Preparation of White Rose Petal Extract
[0064]The prepared dried white rose petal powder was divided into two parts and extracted with ethanol or water.
[0065]First, the ethanol extract was deposited by using 70% ethanol 10 times larger than a volume of the white rose petal powder and extracted for 24 hours at room temperature. An extracted suspension was filtered by a Whattman filter (No. 1) and the filtrate was concentrated under a vacuum at 50° C. and freeze-dried to obtain the extract. Samples obtained from the ethanol extract were continuously fractioned through n-hexane ethyl acetat (EA), chloroform, n-butanol (BuOH), and distilled water, and the obtained fractions were dried by an evaporator. Then, the obtained products were used for the experiments.
example 3
Analysis of Collagen Matrix Metalloproteinase-1 Inhibitory Effect of White Rose Petal Extract
[0066]As part of measuring a wrinkle alleviation effect of the white rose petal extract, effects of inhibiting activity of collagen matrix metalloproteinase-1 (MMP-1) of the extracts were measured with reference to the guideline of “beauty-related functional tests (pp. 821-858)” of Korea health official compendium research society foundation.
[0067]When simply describing the effect, 25 mg of bovine tendon collagen was dissolved in a 0.05 M tris(hydroxymethyl)-methyl-2-aminoethane sulfonate (TES) buffer and then left for 15 minutes at 37° C. Next, a test material for each concentration was added and then additionally reacted with collagenase type-1 for 5 hours at 37° C. 0.2 mL of a reaction solution was taken and added to 1 mL of a buffer [14% ninhydrin in methyl cellosolve (2-methoxyethanol)+0.2 M sodium citrate with 0.71 mM stannous chloride]. The reaction solution was boiled for 20 minutes,...
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