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Method for verifying pomegranate rind extract

A pomegranate peel extract and extract technology, which is applied in the identification field of pomegranate peel extract, can solve the problems of difficult identification of appearance, no relevant literature reports, and distinction between two products, so as to achieve strong operability and quality assurance , good separation effect

Inactive Publication Date: 2012-05-23
成都华高瑞甜科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The color, smell, and physical and chemical properties of the two products are similar in liquid state, and it is difficult to distinguish them in appearance
There is no effective method to distinguish the two products, and there are no relevant literature reports

Method used

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  • Method for verifying pomegranate rind extract
  • Method for verifying pomegranate rind extract
  • Method for verifying pomegranate rind extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Detection method:

[0044] 1) Main instruments and reagents: HP1100 liquid chromatograph

[0045] Chromatographic methanol is chromatographically pure, chromatographic water is double-distilled water, and the rest are analytically pure.

[0046] 2) Instrument conditions

[0047] Detector: Diode Array Detector

[0048] Mobile phase: methanol: 0.2% phosphoric acid aqueous solution (volume ratio 45:55)

[0049] Flow rate: 1.0ml / min

[0050] Column temperature: room temperature

[0051] Chromatographic column: Kromasil KR100-5C18 250×4.6mm E17580

[0052] Injection volume: 10ul

[0053] 3) Standard curve drawing: Weigh 49.72 of the ellagic acid reference substance into a 100ml volumetric flask, dissolve in methanol and make to volume. Pipette 0.5ml, 1ml, 2ml, 3ml, 4ml, and 5ml of the prepared ellagic acid reference solution into 10ml volumetric flasks, add methanol to dilute to the mark, and inject samples to obtain the ellagic acid standard curve. (see attached ...

Embodiment 2

[0066] Embodiment 2 The mensuration of chromatogram separation degree of the present invention

[0067] Determination according to the determination method of separation:

[0068] Separation: R = 2 ( T R 2 - T R 1 ) W 1 + W 2

[0069] Among them: T R1 is the retention time of the previous peak among adjacent peaks; T R2 is the retention time of the next peak in the adjacent peaks;

[0070] W 1 and W 2 This is the peak width of two adjacent peaks.

[0071] The measurement results: image 3 , Image 6 , Figure 9 , ...

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Abstract

The invention relates to a method for checking the pomegranate peel extractive, wherein it uses HPLC method; and the quality control method of invention is simple that it uses liquid post and DAN separation to check two character peaks of extractive while the peak is 5 times higher than the sub extractive; the separation between two peaks is significant, and the peak time is stable; but the pomegranate peel extractive can not repeat the second character peak.

Description

technical field [0001] The invention relates to a method for identifying medicinal material extracts, in particular to a method for identifying pomegranate peel extracts. Background technique [0002] Pomegranate extract has strong antioxidant properties, and its main components are polyphenols. Current pharmacological research shows that pomegranate extract can improve myocardial ischemic anemia, lower blood pressure, and inhibit the activity of angiotensin II converting enzyme, and has broad prospects in the treatment of cardiovascular diseases. At the same time, it has the effects of improving gastric ulcer symptoms and anti-cancer effects. There are researches on anti-inflammation and treatment of Alzheimer's disease abroad. [0003] Most of the pomegranate peel extract products currently on the market are pomegranate peel extracts containing 40% ellagic acid, and there are two sources, one is the high-content ellagic acid product prepared from gallnuts and the crude e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P1/04A61P35/00A61P9/10A61P9/12G01N30/02G01N33/15
Inventor 阳明福李芬郑小强李玉元
Owner 成都华高瑞甜科技有限公司
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