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Gene chip, detection method, and kit for detecting virus infected by oxen

A gene chip and infectious disease technology, applied in the field of gene chip detection of bovine infectious disease virus, can solve the problems of small number of operation sequences, low degree of automation, low detection efficiency, etc., and achieve simple operation, high accuracy and strong repeatability Effect

Inactive Publication Date: 2007-05-09
IPE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In general, because the chip technology immobilizes a large number of probes on the support at the same time, it can detect and analyze a large number of sequences in the sample at one time, thus solving the problem of complicated operation, low automation and sequence of operations in traditional nucleic acid blot hybridization technology. The number is small, the detection efficiency is low, etc.

Method used

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  • Gene chip, detection method, and kit for detecting virus infected by oxen
  • Gene chip, detection method, and kit for detecting virus infected by oxen
  • Gene chip, detection method, and kit for detecting virus infected by oxen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Design and synthesis of rabies virus probe

[0042] 1. Design

[0043] Literature search and the comparison of the National Center for Biological Information (NCBI) in the United States to obtain the rabies virus candidate specific probe sequence, compared in the NCBI database, if the sequence has high intra-species conservation and high inter-species specificity, it can be designed as probe.

[0044] The following are the two nucleic acid sequences of the selected rabies virus genome:

[0045] Fragment 1

[0046] CCGGAGAAGT AGTCCTCGTC ATCAGAGTTG ACAGTTCCAT CATCCGCCAA TGCCACATCA GTCTTTGTCA

[0047] GTTCAGCCGC CTCATATTCT TGAAGTTCTT TCTCATCTCT GAAGAATCTT CTTTCAAATG TCCCTTTCCC

[0048] GAAGAATTCC TCTCCCAGAT AGCCCCCTAA AACAGACATC TCATGAGGAG CACATGCGGC GATAACTGTT

[0049] GCATTTAGGG ATCTGACTTG ACCCATATAG CATCCAACAA AGTGAATGAG ATTAAACACG TGACCAACGG

[0050] CATTCGATGA ATAAGGAGAC

[0051] TTCCCACTCA AGCCTAGTGA ACGGAAGTGA ATGAAATACG AGTGAGGAAC

[0052] Fragment 2

[00...

Embodiment 2

[0089] Design and Synthesis of Probes for Bovine Diarrhea Virus

[0090] Method is the same as embodiment 1, two nucleotide sequences of the bovine diarrhea virus genome of choosing:

[0091] Fragment 3

[0092] TTGGATG GCTGAAGCCC TGAGTACAGG GTAGTCGTCA GTGGTTCGAC GCC

[0093] TTAGGATCTGAGGCCTC GAGATGCCAC GTGGACGAGG GCATGCCCAC AGCA

[0094] CATCCT ATTCCGGACGAGGGTCGCCC GGAAGAAAGC GGTTGTACTA ACCGT

[0095] TATGG ACACGGCCTG ATAGG

[0096] Fragment 4

[0097] GTCCAACGGG CGATGTTCCA AAGAAACGTG AGTAGGAGCC TGCACGGGAT TTGGCCTGAAAGAATATGTG

[0098] CCGGTGTCCC CTCCCATCTA GCCACTGATA CTGAACTGAA AACGATTCTTGGTATGATGG ATGCAAGTGA

[0099] GAAGACCAAT TATACCTGTT GCAGGCTCCA GCGCCACGAATGGAACAAAC ATGGCTGGTG CAACTGGTTT

[0100] AATATAGAAC CTTGGCTTCT CCTAATGAACAAAACCCAGG CCAATCTGAC CCAGGGCCAA CCACCAAGAG

[0101] AGTGTGCAGT CACATGTAGA

[0102] TATGATAGGG ACTCTGACTT AAATGTGGTG ACGCAGGCCA GGGACAGCCC

[0103] Fragment 3 was split into the following 6 oligonucleotide sequences:

[0104] oligo 13...

Embodiment 3

[0125] Design and Synthesis of Foot-and-Mouth Disease Virus Probe

[0126] Method is with embodiment 1, two nucleotide sequences of the foot-and-mouth disease virus genome of choosing:

[0127] Fragment 5

[0128] CGGGGCCAAAAGCCACGTCCAGATGGACCCACCATGTGTG CAACCCCAGC ACGGCAACCT

[0129] TACTGCGAACACCACCTAACGTGACACTGATACTGGTAC TCGGTCACTG GTGACAGACT

[0130] AAGGATGCCCTTCAGGTACCCCGAGGTAACACGGGACACT CGGGATCTGAGAAGGGGATT

[0131] GGGACTTCTTTAAAAGTGTCCAATTTAAAAAGCTTCTATG TCTGAATAGG CGACCGGAGG

[0132] CCGGCGCCTTTCCATTACCTACTACTAAAATCCATGAATACGACTGACTGTTTTTATCGCTCTGCTACACAT

[0133]TCTCAGAGAGATCAAAGCAC TGTTTCTGTCGCGAACACAA

[0134] Fragment 6

[0135] GATCTCAA CGACCCCGGT AAGTACAAGG AGGCCAAGGA ATGGCTGGAC AACG

[0136] CGCGCC AAGCGTGTCT GAAGAGCGGG AACGTCCACA TTGCCAACCT GTGC

[0137] AAAGTG GTTGCTCCAG CGCCCAGCAA GTCGAGGCCC GAACCAGTGG TCGTG

[0138] TGTCT TCGCGGCAAA TCCGGCCAAG GGAAGAGTTT CCTCGCGAAC GTTCTC

[0139] GCACAGGCAATCTCCACCCACTTCACTGGCAG

[0140] Fragment 5 was split i...

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Abstract

This invention relates to a gene chip for detecting viruses of bovine infectious diseases. The gene chip comprises a solid carrier and an oligonucleotide probe immobilized on the solid carrier. The oligonucleotide probe is DNA fragment selected from the nucleotide sequences of bovine rabies virus, bovine viral diarrhea virus, bovine foot and mouth disease virus, rinderpest virus and bovine bluetongue virus. This invention also relates to a method for preparing the gene chip and utilizing the gene chip for detecting the five viruses. The method comprises: synthesizing the probe, preparing the gene chip, and hybridizing with a treated sample. The gene chip, together with sample treatment solution, hybridization reagent, color-development reagent and specification, constitutes the test kit for detecting viruses of bovine infectious diseases. The gene chip can detect the five viruses simultaneously, and has such advantages as easy operation, high accuracy and good repeatability.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a gene chip for detecting bovine infectious disease viruses. A chip preparation method, a detection method and a detection kit. Background technique [0002] Rabies is caused by rabies virus, also known as hydrophobia, characterized by high central nervous system excitement and severe disturbance of consciousness, paroxysmal excitement and shock movements, and finally death due to respiratory nerve paralysis. The case fatality rate is extremely high, and humans and various livestock and poultry are susceptible. In my country, the incidence rate continues to rise. At present, the key epidemic areas in the world are still in Asia, mainly in Southeast Asian countries. In recent years, the world's epidemic trend has also increased. [0003] Bovine viral diarrhea is an infectious disease of cattle and sheep caused by mucosal disease viruses. It is characterized by erosion an...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/70
Inventor 周骋马立人
Owner IPE BIOTECHNOLOGY CO LTD
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