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Bioreactor for constructing tendon from tissue engineering

A bioreactor and tendon technology, applied in the direction of microorganisms, tissue culture, artificial cell constructs, etc., can solve the problems of not being able to observe the reaction of cells and biological materials, nude mice without perfect immune function, and not conforming to physiological characteristics, etc., to achieve Improved local microenvironment, good strength and toughness, and good mechanical properties

Inactive Publication Date: 2007-05-09
SHANGHAI TISSUE ENG LIFE SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, nude mice do not have perfect immune function, and cannot observe the body's response to cells and biomaterials, and the tissue is not subjected to stress during the formation process, and the resulting tissue-engineered tendon does not conform to physiological characteristics.
[0004] In the prior art, autologous tenocytes are also used to construct tissue-engineered tendons, and in situ to repair chicken toe flexor tendon defects successfully, but the use of autologous tenocytes as seed cells for tissue-engineered tendons still requires larger tendon tissues, resulting in new damage

Method used

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  • Bioreactor for constructing tendon from tissue engineering
  • Bioreactor for constructing tendon from tissue engineering
  • Bioreactor for constructing tendon from tissue engineering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Preparation of bioreactor

[0062] As shown in Figure 1, the bioreactor of the present embodiment includes a cell culture room, a control system, a liquid change control system and a parameter measurement system, wherein the cell culture room is connected to the control system, the liquid change control system and the parameter measurement system respectively .

[0063] The bioreactor is made of completely transparent organic materials, and its outer structure measures 450 mm in length, 130 mm in height and 350 mm in width. The outer dimensions of the cell culture chamber in the reactor are 140 mm in length, 60 mm in height and 90 mm in width. The number of reactors can be reasonably combined according to specific requirements to form multiple different and relatively independent single culture chambers placed in the incubator. The tension display can be accurate to 0.00 kg, and the measurement range is 0-4 kg. The displacement measurement can be accurate to 0.01 mm, ...

Embodiment 2

[0065] Method for culturing tenocytes in a bioreactor

[0066] (1) Under sterile conditions, cut all the deep flexor tendons of Leghorn chicken toe 3-4cm, cut into 1×2×2mm 3 The large and small tissue pieces were washed twice with phosphate buffer solution (PBS, containing 100 U / ml each of penicillin and streptomycin), and the tissue pieces were cultured adherently;

[0067] (2) The tenocytes were passed to the second passage, and the cells were collected by digesting with 0.25% trypsin;

[0068] (3) Arrange 20 mg of PGA in parallel into bundles, 3.5 cm long and 0.3 cm in diameter;

[0069] (4) Use the above cells and culture medium to make a concentration of 5.0×10 7 / ml of cell suspension, then mixed with PGA to form a complex, fixed in the culture chamber of the reactor, and placed in an incubator at 37°C and 5% carbon dioxide concentration for 4 hours;

[0070] (5) Then add DMEM culture fluid containing 20% ​​fetal bovine serum and continue to place it in the incubator ...

Embodiment 3-6

[0073] Bioreactor culture of tenocytes

[0074] Example 3

[0075] Number of stretches per day

[0076] The measurement results of the tendon grafts in Examples 3-6 are similar to those in Example 2, and the tensile strengths at 8 weeks are respectively 1.5997±0.0904MPa.

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Abstract

This invention relates to a biological reactor for constructing tissue engineered muscle tendon. The biological reactor is composed of a cell culture chamber, a control system, a liquid-change control system, and a parameter measurement system. The biological reactor can apply a pulse tension on the composite of cell and degradable material. The muscle tendon constructed by the biological reactor has a strain range of 4-15%, thus has excellent biological and mechanical properties. The biological reactor can also be used for culture of other tissues and cells.

Description

technical field [0001] The present invention relates to a bioreactor, in particular to a bioreactor for constructing tissue-engineered tendons. Background technique [0002] After tendon injury or defect, its surgical repair and functional reconstruction are very important research topics in surgery. Clinically, tendon defects exceeding 3 cm cannot be sutured directly. At present, autologous tendon transplantation or allogeneic tendon transplantation, artificial synthetic materials, etc. are often used. However, the application of the above methods has the disadvantages of limited donor sources and new damage, and is not easily accepted by patients. Tissue engineering technology provides a new way for the clinical repair of tendon tissue defects, however, in the static culture of tendon, the cell proliferation ability decreased, the extracellular matrix synthesis decreased, and the PGA (polyglycolic acid) material began to develop within 1-2 weeks Degradation and discontinu...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12N5/06A61F2/08G01N33/483C12N5/071
Inventor 曹谊林李宏刘伟崔磊
Owner SHANGHAI TISSUE ENG LIFE SCI
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