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Method for preparing a welan gum

A warm rubber and medium technology, applied in the field of microbial fermentation and extraction, can solve the problems of high concentration of calcium ions, complex process steps, high cost of medium, etc., and achieve easy automatic control, reasonable process route setting, and low cost Effect

Active Publication Date: 2006-10-11
HEBEI HENBO BIO TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The welan gum prepared by this method has good hydration properties and temperature resistance (invariance within 300 degrees Fahrenheit), but the data about its viscosity shows that the welan gum prepared by this method has a very low viscosity (less than 100cp ), and the fermentation conditions (pH) need to be changed during the fermentation process, the process steps are complex, the cost of the medium is high, and there is no data record and text description that can tolerate high concentrations of calcium ions

Method used

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Embodiment 1

[0048] The preparation method of Wenlun gum, including the fermentation step, is to inoculate the production strain of Wenlun gum in a sterile medium containing carbon source, nitrogen source, inorganic salt and water for fermentation, and the fermentation process is carried out at a temperature of 29°C The aerobic fermentation is carried out under the condition of pH 6.2-6.7, the production strain of the method is Sphingomonas sp CGMCC1561, and the final fermentation product is extracted to obtain Wenlun gum.

[0049] The fermentation process includes the following process steps:

[0050] A, the original bacterial classification is made the slant bacterial classification;

[0051] B, the slant bacterial classification prepared in the A step is made seed liquid through expanding culture;

[0052] C, the seed liquid prepared in the B step is pressed seed liquid: fermentation medium is that 8% inoculum size inserts fermentation medium and inserts fermentation medium and ferment...

Embodiment 2

[0081] The preparation method of Wenlun gum, including the fermentation step, is to inoculate the production strain of Wenlun gum in a sterile medium containing carbon source, nitrogen source, inorganic salt and water for fermentation, and the fermentation process is carried out at a temperature of 33°C, The aerobic fermentation is carried out under the condition of pH 6.5, and Sphingomonassp CGMCC1561 is selected as the production strain, and the final fermentation product is extracted to obtain Wenlun gum.

[0082] The fermentation process includes the following process steps:

[0083] A, the original bacterial classification is made the slant bacterial classification;

[0084] B, the slant bacterial classification prepared in the A step is made seed liquid through expanding cultivation;

[0085] C, the seed liquid prepared in the B step is pressed seed liquid: fermentation medium is that 9% inoculum size inserts fermentation medium and inserts fermentation medium and ferme...

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Abstract

This invention belonging to fermentation microbiology and extraction is a preparation method of mild-wheel gel. We use Sphingomonas sp CGMCC1561 as engineering strain, ferment under the conditions of 25-36deg C and PH 6.2-6.7, and extract mild-wheel gel from fermentation product. This invention overcomes many difficulities in previous methods, such as low adhesiveness (lower than 100cp), PH condition change in fermentation, high cost on medium, low durability of highly-densed calcium ion and so on.

Description

technical field [0001] The invention belongs to microbial fermentation and extraction, in particular to a preparation method of Wenlun gum. Background technique [0002] WELAN GUM is an industrial-grade microbial polysaccharide, which contains neutral sugar, D-glucose, D=glucuronic acid, L-rhamnose and L=mannose, and glycoside-bonded acetyl esters base. Jansson PE, Lindberg B and Widmalm G (1985) described the structure of this polysaccharide in "Carbohydrate Research" (139, 217-223), and only the United States has achieved industrial production. [0003] US Patent No. 4,963,668 discloses a low-viscosity welan gum, the viscosity of its 5% aqueous solution is only 100 cp, which can be used in cement products. [0004] The invention patent No. 92102564.5 relates to the method of preparing rapid hydration Welan Gum (WELANGUM). The method is to ferment the S-130 strain and make the S-130 strain aerobically ferment at a pH value of about 6.5-7.5. During the fermentation proces...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12P19/04C12P1/00
Inventor 张禹张国佩
Owner HEBEI HENBO BIO TECH
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