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Method for preparing injectable polyletic acid micro-carrier/chitosan hydrogel composite scaffold

A technology of composite scaffold and polylactic acid, which is applied in the direction of coating, etc., can solve the problems of low strength, wandering, and difficult molding, etc., and achieve the effect of improving strength, mild implementation conditions, and simple operation process

Inactive Publication Date: 2006-07-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Injectable hydrogel scaffolds are mainly formed in vivo through sol-gel transition, which has good cell compatibility and easy molding, but has low strength and rapid degradation
Injectable cell microcarriers need to use liquid as a transport carrier to inject into the damaged part and build them up in the body. It is easy to operate, but it is not easy to shape, and there is a problem of wandering in the body.
At present, both at home and abroad are basically focusing on the improvement of single-component performance, and there is no combination of the two to obtain optimal performance.

Method used

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  • Method for preparing injectable polyletic acid micro-carrier/chitosan hydrogel composite scaffold
  • Method for preparing injectable polyletic acid micro-carrier/chitosan hydrogel composite scaffold
  • Method for preparing injectable polyletic acid micro-carrier/chitosan hydrogel composite scaffold

Examples

Experimental program
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Effect test

example 1

[0024] Weigh 800mg of chitosan (CS) and place it in a 250ml Erlenmeyer flask, add 100ml triple distilled water and 420μl MA (0.48mmol), after the CS is completely dissolved, add 930mg EDAC (0.48mmol), then stir the reaction at room temperature 24h. In order to remove unreacted MA and other small molecular products, the reaction mixture was placed in a dialysis bag with a cut-off molecular weight of 10,000 Da, dialyzed in a large amount of triple-distilled water at room temperature for 3 days, and the triple-distilled water was changed 2 to 3 times a day. Finally, the liquid was frozen and freeze-dried to obtain MA-grafted chitosan (CM). The CM yields are all greater than 90%, and the MA grafting amount is about 23%, see figure 1 ; Swellable in water. Dissolve the above 400mg CM in 50ml triple distilled water containing 210μl LA (0.2mmol), and add 460mgEDAC (0.24mmol) after complete dissolution. After the mixture was stirred at room temperature for 24 hours, the reaction mix...

example 2

[0026] Weigh 800mg of chitosan and place it in a 250ml Erlenmeyer flask, add 100ml triple distilled water and 420μl MA (0.48mmol), after the CS is completely dissolved, add 232.5mg EDAC (0.12mmol), then stir the reaction at room temperature for 24h . In order to remove unreacted MA and other small molecular products, the reaction mixture was placed in a dialysis bag with a cut-off molecular weight of 10,000 Da, dialyzed in a large amount of triple-distilled water at room temperature for 3 days, and the triple-distilled water was changed 2 to 3 times a day. Finally, the liquid was frozen and freeze-dried to obtain MA-grafted chitosan (CM). The CM yields are all greater than 90%, and the MA grafting amount is about 11.74%, see figure 1 .

example 3

[0028] 400 mg of CM obtained in Example 1 with a grafted MA amount of about 23% was dissolved in 50 ml of three-distilled water containing 210 μl of LA (0.2 mmol), and 115 mg of EDAC (0.06 mmol) was added after complete dissolution. After the reaction mixture was stirred at room temperature for 24 hours, it was placed in a dialysis bag with a cut-off molecular weight of 10,000 Da, dialyzed in a large amount of triple-distilled water at room temperature for 3 days, and the triple-distilled water was changed 2 to 3 times a day. Finally, the liquid was frozen and freeze-dried to obtain MA and LA grafted chitosan (CML). The yield of CML is greater than 90%, and the amount of LA grafting is about 43.2%, see figure 2 .

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Abstract

The invention discloses a method for preparing injection polylactic acid microcarrier / chitose aqueous gel composite cradle, grafting the methacrylic acid and lactic acid onto the chitose molecular chain in order with carbodiimide condensation method, and getting the polymerized water soluble chitose derivative, mixing the polylactic acid microcarrier and chitose aqueous gel, adding thickening agent konjak glucomannan to increase the viscosity of the prepolymer solution to guarantee the polylactic acid microcarrier suspension, which is facilitate for the injection operation, employing the redox initiating system of ammonium peroxodisulfate and tetramethylethylenediamine to make the composite mentioned above gelify to composite cradle of cell microcarrier / chitose aqueous gel under body temperature in home position. The process of this invention is simple, the prepared composite cradle not only solves the problems of uneasy to shape and mobile of the injection microcarrier in the body, but also can distinctively increase the aqueous gel intensity. The composite cradle is non toxic, biodegradable and biological compatibility, and is expected to be used for injection cradle in tissue engineering.

Description

technical field [0001] The invention relates to a preparation method of an injectable polylactic acid microcarrier / chitosan hydrogel composite support, in particular to an injectable support used in tissue engineering. technical background [0002] Injectable scaffolds are composed of cells and a fluid, biocompatible material, and are directly injected into the defect site of the body through a syringe. The material can form a scaffold in situ with certain mechanical strength, certain shape and exchangeable with body fluids, in which cells grow and eventually form tissues. The material can also be directly injected into the body, and the cells in the surrounding tissue of the injection can be used to expand, grow and proliferate to form a tissue. Due to the characteristics of minimal invasiveness, in vivo culture environment and low cost, injectable stents have important clinical application value and good development prospects. Injectable scaffolds are mainly divided into...

Claims

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Application Information

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IPC IPC(8): A61L27/34
Inventor 高长有洪奕沈家骢
Owner ZHEJIANG UNIV
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