Young schistosomiasis cell style vaccine for preventing from schistosomiasis disease

Abstract

A schistosomulum cell type vaccine for preventing schistosomiasis is prepared through collecting the schistosomulums from the rabit infected by Japanese schistosome for 14-24 days, fast cutting in aseptic mode, digestion by pancreatin, taking supernatant, adding Ca or Mg ion type D-Hankí»s liquid to terminate the digestion to obtain living cells, freezing them to obtain the dead cells and using said living cells or dead cells as vaccine.

Description

technical field [0001] The invention relates to a vaccine product for immunization and prevention of schistosomiasis, in particular to a childhood worm cell vaccine. Background technique [0002] Schistosomiasis is a major tropical disease in the world, threatening 600 million people. Among them, schistosomiasis japonicum is seriously prevalent in our country, which is a zoonotic parasitic disease. Because the epidemic and transmission of the disease among humans and animals is caused by the natural environment, the current prevention and control measures cannot meet the requirements of controlling its epidemic and transmission. [0003] So far, domestic research on (Japanese / Mansoni) schistosomiasis vaccine types includes: 1. Live attenuated vaccine: live cercariae of schistosomiasis are used to vaccinate / immunize animals after being irradiated with radiation. 60% to 90% (worm reduction rate) protective immunity, but very impractical; 2. Insect vaccine: including immunizi...

AI Technical Summary

Problems solved by technology

Because the epidemic and transmission of the disease among humans and animals is caused by the natural environment, the current prevention and control measures cannot meet the requirements of controlling its epidemic and transmission.

[0003] So far, domestic research on (Japanese / Mansoni) schistosomiasis vaccine types includes: 1. Live attenuated vaccine: live cercariae of schistosomiasis are used to vaccinate / immunize animals after being irradiated with radiation. 60% to 90% (worm reduction rate) protective immunity, but very impractical; 2. Insect vaccine: including immunizing animals with tissues or soluble compound components of Schistosoma adult or juvenile worms, which can hardly induce the host to produce resistance Attack infection immunity; 3. Natural molecular vaccines: isolate effective natural ingredients from schistosome worms or eggs, add adjuvants and use them to immunize animals, which can only induce partial protective immunity, and immature egg molecules are used The antigen has anti-schistosome reproduction or egg embryo development effect, but the anti-challenge infection is poor; 4. Recombinant vaccine: Antiserum is obtained after screening and identifying effective antigen molecules or irradiating cercariae to immunize animals, and screening the cDNA library of schistosomiasis , using genetic recombination technology to express recombinant / fusion proteins to immunize animals, the protective immune effect is within 40% (worm reduction rate); 5. Gene vaccine: also known as DNA vaccine, screened by library (including polypeptide library) The identified fragments are constructed into eukaryotic expression vectors to directly immunize animals, but the protective immune effect is not ideal; 6. Polypeptide vaccines: design and synthesize molecular antigen peptides according to the epitope sequences of effective molecules, and generally combine multiple polypeptide molecules It is used to immunize animals to obtain better protection, but the ideal result is not achieved; 7. Multi-epitope vaccine: use the B cell and T cell epitopes of effective vaccine molecules to synthesize cDNA fragments, and construct recombinant or genetic vaccines for use The effect of immunizing animals is unsatisfactory; 8. Compound vaccine: also known as "cocktail" vaccine, multiple vaccine candidate molecules are combined to immunize animals to observe the effect of anti-challenge infection. Ideal; 9. Heterologous vaccine: use Fasciola hepatica or Trichinella antigen to immunize the host to observe the effect of resisting schistosomiasis attack infection, and the effects are all beyond the requirements of the above-mentioned homologous vaccine; 10. Anti-idiotypic vaccine: to resist disease Animals are immunized with the antibody of the original organism. Anti-Ab2 idiotype antibodies are produced after the animal is immunized with Ab2 as an antigen mimic, which plays a protective role. It is currently being explored.

[0004] The above-mentioned vaccines have the unsafe problems of irradiating live cercariae vaccines and the impracticality of a large amount of waste materials. In addition to the irradiated vaccines, adjuvants need to be added

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