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Quality detection method for compound prepn of red sage and notoginseng

A quality inspection method and technology for compound preparations, which are applied in the directions of medical preparations containing active ingredients, testing pharmaceutical preparations, pharmaceutical formulas, etc., to achieve the effects of accurate method, good separation degree and good recovery rate

Inactive Publication Date: 2006-05-17
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, only the content of a certain type of active ingredients in Salvia miltiorrhiza or Panax notoginseng can be measured separately, so it is of great significance to establish a method that can simultaneously control the content of multiple types of active ingredients in Danshen and Sanqi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Instruments and reagents

[0024] Agilent 1100 series high performance liquid chromatography, including G1312A quaternary gradient pump, G1313A autosampler, G1316A column thermostat, G1315A DAD detector; Chemstation 6.01 chromatographic workstation.

[0025] Compound Danshen preparation: Danshen Tablets (Danshen: Sanqi = 1:1): Hunan Anbang Pharmaceutical Co., Ltd. (batch number: 040803); Compound Danshen Tablets (Danshen: Sanqi = 450:141): Guangdong Baiyunshan Pharmaceutical Factory (batch number: 03121024), Nanjing Tongrentang Pharmaceutical Co., Ltd. (batch number: 040602), Zhejiang Huqingyutang Pharmaceutical Co., Ltd. (batch number: 050308); compound Danshen dripping pills: Tianjin Tasly Pharmaceutical Co., Ltd. (batch number: 20020921, 20030604, 20040303 ).

[0026] Reference substances: protocatechualdehyde, salvianolic acid B, tanshinone IIA, notoginsenoside R 1 , Ginsenoside Rg 1 , Ginsenoside Rb 1 Purchased from China Institute for the Control of Pharmac...

Embodiment 2

[0041] The detection wavelengths are 203nm and 281nm, and the notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 are determined at 203nm, and protocatechualdehyde, salvianolic acid B, cryptotanshinone and tanshinone IIA are determined at 281nm. The recording time is 80min. Others are with embodiment 1. The experimental results are shown in Table 2:

[0042] medicine

[0043] ND: not detected

experiment example 3

[0045] Methodological investigation:

[0046] 1. Linear relationship

[0047] Accurately weigh the reference substances (1) protocatechualdehyde 2.63 mg, (2) notoginsenoside R 1 7.67mg, (3) Ginsenoside Rg 1 12.30mg, (4) Salvianolic acid B 2.54mg, (5) Ginsenoside Rb 1 12.53mg, (6) cryptotanshinone 2.75mg and (7) tanshinone IIA 2.69mg, put in a 5ml brown volumetric flask, add 70% methanol (cryptotanshinone and tanshinone IIA plus methanol) to dissolve and constant volume, as the reference substance stock solution. Precisely draw a certain amount of reference substance stock solution, put them in a brown volumetric flask to mix and add 70% methanol to constant volume to form 10 concentrations of mixed reference substance solutions, the concentrations are: (1) 1.32, 2.63, 5.26, 7.89, 13.15 , 26.30, 52.60, 105.20, 157.80, 210.40 μg / ml, (2) 15.34, 30.68, 38.35, 46.02, 61.36, 122.72, 184.08, 245.44, 306.80, 368.16 μg / ml, (3) 29.52, 94.048, 75 , 98.40, 196.80, 295.20, 393.60, 492...

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PUM

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Abstract

The present invention relates to Chinese medicine quality detecting technology, and is especially the quality detection method for compound preparation of red sage and notoginseng. HPLC-DAD process is adopted to measure the contents of protocatechuic aldehyde, salvianolic acid B, cryptotanshinone, neotanshinone IIA, arasaponin R1, ginsenoside Rg1 and ginsenoside Rb1 in compound red sage preparation simultaneously. The process is simple, precise, repeatable and reliable, and may be used in the quality control of compound red sage preparation.

Description

Technical field: [0001] The invention relates to the field of quality detection of traditional Chinese medicines, in particular to a quality detection method of a compound preparation containing salvia miltiorrhiza and notoginseng medicinal materials. Background technique: [0002] Preparations containing Danshen and Panax notoginseng, commonly known as compound Danshen preparations, are widely used clinically to treat cardiovascular diseases. The common ones are Compound Danshen Tablets, Compound Danshen Dripping Pills, Danqi Tablets, Guanxin Danshen Tablets, etc. [0003] Salvia miltiorrhiza and Panax notoginseng are the two main components of the compound salvia miltiorrhiza preparation. Modern research shows that the active ingredients of Salvia miltiorrhiza are mainly fat-soluble diterpene quinones (such as tanshinone IIA, etc.) and water-soluble active ingredients of salvianolic acids (such as salvianolic acid B, etc.). Thrombosis, anti-oxidation and protection of ca...

Claims

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Application Information

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IPC IPC(8): A61K36/53A61K36/258A61P7/02A61P9/10G01N30/02G01N33/15
Inventor 李萍韦英杰李松林
Owner CHINA PHARM UNIV
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