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Himematsutake water soluble polysaccharide and its preparing process and use

A water-soluble polysaccharide and preparation technology, which is applied in the direction of food preparation, application, organic active ingredients, etc., can solve the problems of low alcohol precipitation efficiency, unstable quality, unsatisfactory pharmacological activity and clinical curative effect, etc.

Inactive Publication Date: 2005-08-03
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, when this process is used to prepare Agaricus blazei polysaccharides, the alcohol precipitation efficiency is low, and the content of impurities, especially small molecular impurities, is high, so only crude polysaccharides can be prepared, and the content of effective polysaccharides is relatively low
In addition, this process also has various defects such as time-consuming, poor reproducibility, and unstable quality, which directly lead to unsatisfactory or unstable pharmacological activity and clinical efficacy.

Method used

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  • Himematsutake water soluble polysaccharide and its preparing process and use
  • Himematsutake water soluble polysaccharide and its preparing process and use
  • Himematsutake water soluble polysaccharide and its preparing process and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0154] Example 1 Alcohol Extraction Process Optimization for Removing Small Molecular Components

[0155] 1. Orthogonal experimental optimization of extraction process:

[0156] (1) Orthogonal experimental design:

[0157] A: Ethanol concentration

[0158] A 1 0%; A 2 15%; A 3 30%; A 4 45%; A 5 60%; A 6 75%; A 7 85%; A 8 95%.

[0159] B: sample (kg) / solvent volume (liter)

[0160] B 1 1:6; B 2 1:8

[0161] C: extraction time (h)

[0162] C 1 1.5;C 2 3.0

[0163] D: number of extractions

[0164] D. 1 2;D 2 3.

[0165] Design L based on the above factors and levels 16 (8×2 8 ) is shown in Table 6.

[0166] 1 2 3 4 5 6 7 8 9

[0167] (2) Judgment indicators and measurement results:

[0168] The evaluation and judgment indexes of this orthogonal experiment are the content of trehalose in the extract and the total amount of trehalose, glucose and mannitol. The contents were determined by HPLC-ELSD method. The experimental result...

Embodiment 2

[0175] Example 2. Preparation process and optimization of water-soluble polysaccharide after alcohol extraction and impurity removal of Agaricus blazei

[0176] 1. Establishment of index component content method

[0177] The classic polysaccharide determination method is to measure the total sugar content first, and then subtract the reducing sugar. Since Agaricus has been extracted with 75% ethanol, the small molecular reducing sugar has been extracted completely, so the polysaccharide content is directly determined by the phenol sulfuric acid method.

[0178] (1) Standard curve and linear range

[0179] Accurately weigh 20.0 mg of trehalose in a 500 ml volumetric flask, add water to the mark, and mix well.

[0180] Draw standard solution 0.4ml, 0.6ml, 0.8ml, 1.0ml, 1.2ml, 1.4ml, 1.6ml and 1.8ml respectively, make up to 2.0ml with water, then add 1.0ml of 6% phenol and 5.0ml of concentrated sulfuric acid, Stand still for 10 minutes, shake well, measure the optical density a...

Embodiment 3

[0248] Embodiment 3. The water extraction ultrafiltration process of Agaricus blazei water-soluble polysaccharide

[0249] Agaricus blazei fruiting body coarse powder 1.0Kg, extract with 10 times the amount (weight ratio) of water, extract 2 hours each time, extract continuously 2 times, and combine the extracts. Add 5% ZTC1+1 natural clarifier to the extract, stir well, let stand to cool, and filter by centrifugal filtration to obtain a clarified extract. Pump the extract into an ultrafilter, and perform ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 4,000. After several cycles, collect the unpassed part on the membrane and concentrate it to a thick extract (specific gravity ~ 1.10) with a rotary evaporator. , then dried in a vacuum drying oven and pulverized to obtain 68g of Agaricus blazei water-soluble polysaccharide.

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Abstract

The present invention belongs to the field of food and medicine technology, and especially water soluble Agaricus blazei murrill polysaccharide extracted from Agaricus blazei murrill sporophore or mycelium and its preparation process. The extract has molecular weight over 10,000, and is prepared through one of two extraction process. One of the extraction process includes ethanol extraction to eliminate water soluble small molecular weight impurity, water extraction to obtain water soluble polysaccharide and direct concentration of the water extracted matter; and the other includes hot water extraction, ultrafiltration with ultrafiltering film of intercepting molecular weight 4,000, and concentration of the matter on the ultrafilter membrane. Experiment shows that the extract has obvious tumor cell growth inhibiting effect, obtain immune regulating function and no toxic side effect, and may be used in preparing medicine and functional health food for preventing and treating relevant diseases.

Description

technical field [0001] The invention belongs to the technical field of health food and medicine, and in particular relates to a water-soluble polysaccharide of edible fungus Agaricus blazei and its preparation process as well as its application in health food and medical medicine. technical background [0002] Agaricus blazei, also known as Brazil mushroom, is a precious edible fungus. In terms of fungal classification, it belongs to Basidiomycotina, Laminomycetes, Sanmycetes, Agaricaceae, and the genus Agaricus blazei. The Latin scientific name is Agaricus blazei Murill. The fungus is native to Brazil, the United States, Peru and other Latin American regions. Japan has been successfully introduced and cultivated in the 1970s, and then spread to Southeast Asia such as Vietnam, Thailand, Indonesia and Taiwan, and introduced to my country in the early 1990s. Now Fujian, Zhejiang and other places have a large number of cultivation. At the same time, the liquid culture technol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L31/00A23L33/125A61K31/715
Inventor 周铜水陈家宽
Owner FUDAN UNIV
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