Composition containing high content conjugated linoleic acid and its preparation method
A technology of conjugated linoleic acid and its composition, which is applied in the field of biomedicine, can solve the problems of low CLA content and poor effect, and achieve the effects of reducing blood fat, reducing body fat, and increasing muscle
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Embodiment 1
[0046] Embodiment 1: the preparation of high-content CLA composition,
[0047] Take 50g of CLA composition with a total CLA content of about 81%, carefully inject it into the top of the prepared φ5×50cm silica gel chromatography column at room temperature, open the valve at the lower end of the chromatography column, and control the flow rate to 5ml per minute. After it completely entered the column bed, it was eluted with n-hexane. Every 100ml of eluate was collected in a round bottom flask, after every 100ml was collected, the samples were mixed and directly injected into gas chromatograph to measure its fatty acid composition. The instrument used is a GC-14C gas chromatograph: its chromatographic column is a 0.25mm×100M, CP-SL 88 capillary column, and the detector is a hydrogen flame ionization detector; the analysis conditions are: injector temperature 250°C, The detector temperature is 260°C, and the column temperature is 230°C.
[0048] According to the measurement res...
Embodiment 2
[0054] Example 2: Effects of compositions with different CLA contents on body weight of obese mice,
[0055] 40 obese mice were randomly divided into 4 groups, wherein the three groups were given the same dose of CLA compositions containing 90% and 80% fatty acid type and 80% ethyl fat type (respectively marked as CLA-90, CLA-80 and CLA-80EE), the other group is the control, continuous 36 days, see the results figure 1 . The body weight of mice ingested CLA-90 was lower than that of other groups, and there were significant differences (P<0.05).
Embodiment 3
[0056] Example 3: Effects of compositions with different CLA contents on blood sugar in diabetic mice,
[0057] Four groups of mice suffering from diabetes were given equal amounts of CLA-90, CLA-80 and CLA-80EE compositions respectively, and their effects on blood sugar concentration are shown in figure 2 . All the CLA compositions in the three groups can lower the blood sugar of the mice. But CLA-90 had the best effect, which was significantly different from CLA-80 and CLA-80EE (P<0.05).
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