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Target directional DNA vaccine for preventing caries and preparation method thereof

A DNA vaccine, anti-caries technology, applied in recombinant DNA technology, DNA/RNA fragments, pharmaceutical formulations, etc., can solve problems such as non-compliance with DNA vaccine requirements

Inactive Publication Date: 2005-03-16
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the vector pCI plasmid of the vaccine contains the ampicillin resistance gene, which does not meet the relevant requirements for the vector backbone of DNA vaccines

Method used

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  • Target directional DNA vaccine for preventing caries and preparation method thereof
  • Target directional DNA vaccine for preventing caries and preparation method thereof
  • Target directional DNA vaccine for preventing caries and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0049] The present invention will be described in further detail below according to accompanying drawing:

[0050] according to figure 1 It can be seen that the professional molecular biology software: DNASIS comprehensive DNA sequence analysis software (Hitachi, Japan), Gene Construction kit 2.0 gene simulation cloning software (Textco, USA) was used to analyze and design the targeted anti-caries DNA vaccine and simulate the whole process of cloning.

[0051] according to figure 2 It can be seen that the targeted anti-caries eukaryotic expression plasmid pGJGLU (patent application number: 02139123.8) and pVAX1 vector are treated with restriction enzymes NheI and NotI; the targeted fusion gene (including CTLA-4-Ig target gene, GLU) is excised and NheI and NotI enzyme-digested pVAX1 vector fragments, gel recovery and purification of the target fragments; the above two purified target fragments at T 4 Ligate overnight at 20-25°C under the action of DNA ligase, transform comp...

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Abstract

The invention discloses a target directional DNA vaccine for preventing caries and preparation method thereof, Escherichia coli JM109 / pGJGLU / VAX.CCTCC No.M204027, the preparation comprises cutting out targeting fusion genes (including CTLA-4-Ig destination gene, GLU fragment) from the fusion dental caries proofing eucaryon expression plasmid pGJGLU with limited enzyme NheI and NotI enzyme cutting treatment, cloning targeting fusion gene to pVAX1 carrier, enzyme cutting has shown the correctness of the construction, which is named pGJGLU / VAX, the transfection of eukaryotic cells CHO cell lines has confirmed that right expression recombination protein can expressed in the eukaryotic cells.

Description

technical field [0001] The invention belongs to the technical field of oral preventive medicine, and more specifically relates to a sequence encoding human cytotoxic T lymphocyte antigen 4 signal peptide, extracellular region and Igγ 1 A DNA anti-caries vaccine of hinge region, constant region fusion gene and mutans glucosyltransferase gene, and the present invention also relates to a preparation method of the targeted anti-caries DNA vaccine. Background technique [0002] Dental caries is a common and frequently-occurring disease that seriously endangers the health of human oral cavity and the whole body. The loss of function, inflammation, pain, and organ diseases caused by dental caries can seriously affect the quality of life, reduce the health quality of the population, and bring great harm to society. Caries is a bacterial infectious disease, and Streptococcus mutans (hereinafter referred to as Streptococcus mutans) is the main cariogeni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P1/02C12N15/62
Inventor 樊明文贾荣郭继华边专陈智彭彬范兵
Owner WUHAN UNIV
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