Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Diploid cell cerebritis B vaccine and purified cerebritis B vaccine, dosage form freeze-drying and water injection

A diploid cell and Japanese encephalitis technology, which is applied in the direction of freeze-drying delivery, antiviral agent, drug delivery, etc., to achieve the effect of improving purity, improving stability and increasing persistence

Active Publication Date: 2005-03-02
崔栋
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But prepare Japanese encephalitis vaccine with this method and there is no report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Diploid cell cerebritis B vaccine and purified cerebritis B vaccine, dosage form freeze-drying and water injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Diploid cells come from the Chinese Center for Disease Control and Prevention, use 38-50 generations, the cell nutrient solution is 199 medium containing 2-10% bovine serum and 25IU / ml gentamicin and 25IU / ml kanamycin, and Adjust the pH to 7.2, cultivate a dense monolayer with a 3L spinner bottle at 37°C, and then amplify according to the seeding ratio of 1:2. When the expansion reaches the production batch, after about 4 days, when the cells grow into a dense monolayer, discard the cells Nutrient solution, wash the cell surface with PH7.2 Earl's solution, inoculate diploid cells with JE virus, and use JE mouse encephalovirus P 3 The second-generation working virus strain (P 3-2 ), virus seed concentration MOI0.05, cell maintenance medium is 199 culture medium containing 0.4-0.5% (W / W) human albumin, pH 7.6, cultivated at 35°C, after 24 hours, replace with fresh cell maintenance medium to continue Cultivate for 2 days, start to harvest the virus, harvest once every 3-4...

Embodiment 2

[0057] Diploid cells come from the Chinese Center for Disease Control and Prevention, use 38-50 generations, the cell nutrient solution is 199 medium containing 2-10% bovine serum and 25IU / ml gentamicin and 25IU / ml kanamycin, and Adjust the pH to 7.2, cultivate a dense monolayer with a 3L spinner bottle at 37°C, and then amplify according to the seeding ratio of 1:2. When the expansion reaches the production batch, after about 4 days, when the cells grow into a dense monolayer, discard the cells Nutrient solution, wash the cell surface with PH7.2 Earl's solution, inoculate diploid cells with Japanese encephalitis virus, and use the second generation of JE virus strain AS14-14-2 to pass on diploid cells Poison species (P 3-2 ), virus seed concentration MOI0.05, cell maintenance medium is 199 culture medium containing 0.4-0.5% (W / W) human albumin, pH 7.6, cultivated at 35°C, after 24 hours, replace with fresh cell maintenance medium to continue Cultivate for 2 days, start to ha...

Embodiment 3

[0061] The JE inactivated vaccine prepared by the method of claim 1 is tested (including giving injections to 12 children aged 4-6 years, and those who have not been vaccinated against JE vaccine are inoculated with 2 needles of JE inactivated vaccine, with an interval of 7-10 days between the two needles. .) proved that the positive conversion rate of neutralizing antibodies was 91.7%, and no side effects occurred.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to cerebritis B vaccine of diploid cell and purified cerebritis B vaccine in the preparation form of freeze dried powder for injection and injection liquid. The purified cerebritis B vaccine is obtained through inoculating cerebritis B virus strain onto diploid cell for human body.

Description

Technical field: [0001] The invention relates to a purified Japanese encephalitis vaccine, in particular to a purified Japanese encephalitis vaccine obtained by inoculating Japanese encephalitis virus seeds on human diploid cells. Background technique: [0002] At present, there are three kinds of Japanese encephalitis vaccines produced in large quantities in the world. The first one is a purified Japanese encephalitis vaccine prepared by grinding, emulsifying, concentrating, and ultracentrifuging the brain tissue of mice infected with JE virus, represented by Japan; It is the inactivated JE vaccine cultured from primary hamster kidney cells and the live JE vaccine from primary hamster kidney produced in China. The cell substrates of these two vaccines are derived from ordinary animals, and there is no guarantee that they will not carry foreign factors; The third is the freeze-dried JE purified and inactivated vaccine produced by the Beijing Institute of Biological Products ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/08A61K9/19A61K39/12A61P31/12
Inventor 崔栋卜海兵
Owner 崔栋
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com