Microorganism oil production method

Abstract

The microbial oil extracting method of the present invention is to inject the composite microbial preparation comprising fermented Bacillus liceni formis X3 liquid and mixed hydrocarbon utilizing bacteria culture solution in the ratio of 1 to 1.5-9 into petroleum deposit via water injection well or to the zone near the well via oil well. The fermented Bacillus liceni formis X3 liquid has lipopeptide surfactant content of 1-4 g / L. The present invention is one kind of microbial oil extracting method of microbial oil displacement or single well gorge and disgorge and has simple construction process, short well closing period, low cost and no pollution to stratum and environment.

Description

Technical field [0001] The technical solution claimed by the present invention relates to a method for improving oil extraction by using bacterial activities, specifically a method for microbial oil extraction. Background technique [0002] Hydrocarbon decomposing bacteria and nutrient solution are injected into the reservoir through the water injection well (the process term is called "microbial flooding") or squeezed from the oil well to the near-well zone (the process term is called "single well huff and puff"). The degradation of crude oil and the effect of its metabolites on crude oil emulsify crude oil to reduce viscosity and increase fluidity. It is a method that has been applied to increase oil production or recovery. However, from the published literature, the various microbial oil recovery methods so far are only limited to the role of hydrocarbon decomposing bacteria alone. US 4,475,590 proposes a method of injecting a large amount of nutrient solution from a water inj...

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Problems solved by technology

However, from the published literature, the various microbial oil recovery methods so far are only limited to the single action of hydrocarbon-decomposing bacteria.

US 4,475,590 proposes a method of injecting a large amount of nutrient solution from the water injection well into the reservoir, the purpose is to stimulate the growth and metabolism of microorganisms inherent in the reservoir to improve the recovery; US 5,163,510 discloses the injection of aerobic bacteria from the production well and then inject The method of oxygen, its purpose is to allow the growth and metabolism of microorganisms better, but the construction is complicated, the cost is too high, and it is not safe; US 4,450,908 proposes the method of injecting polyacrylamide from the water injection well and then injecting microorganisms; US 4,905,761 is A patent on injecting microorganisms and nutrient solution into formations from water injection wells; CN 1038327 proposes to enrich microorganisms from the water produced from each oil well, and then inject the nutrient solution and microorganisms back into the oil reservoir from the original well after laboratory experiments. The disadvantage is that hydrocarbon-decomposing bacteria need to be enriched well by well for experimental evaluation, the reaction time between microorganisms and nutrient solution is long, and the well shut-in time is required to be as long as 20 days; CN 1164607A discloses a method of circulating microbial flooding and recovery

Its technology consists of two aspects. On the one hand, microorganisms are injected into production wells or water injection wells. On the other hand, microorganisms that survive and multiply in the oil layer are extracted from production wells, separated from oil and water, and then the sewage containing bacteria is injected into the oil reservoir. At the same time, depending on the situation, special or the same microorganisms can be added into the water injection well together with the sewage. This method is complex in pipe network reconstruction and construction, and the cost is also high. Due to engineering technical or economic reasons, not all sewage in this system can be used. Inject back into this system; CN 1254788A discloses a kind of microbial compound flooding oil recovery method, it is the oil recovery method that injects microorganism and a set of mature non-microbial oil displacement method through water injection well and combines, for example with chemical profile control, chemical Combination of polymer flooding, foam flooding and three-element compound flooding of alkali / chemical surfactant / polymer; CN 1257154A introduces a method of oil recovery with microbial composite measures, which is to squeeze microorganisms into oil wells Oil recovery methods combined with a well-established set of non-microbial recovery measures in the near-wellbore zone, such as chemical fracturing, water shutoff and nitrogen injection, etc.

[0003] The various microbial oil recovery methods reported in the above literature are limited to the single action of hydrocarbon-decomposing bacteria. Some of these methods are quite complex in construction, some are costly and pollute the formation and the environment, and some are difficult to examine the effect of microbial oil recovery technology.

About adding Bacillus licheniformis (Bacillus licheniformis) X to the culture solution of hydrocarbon-decomposing bacteria 3 The microbial oil recovery method in which the fermented broth is formulated with a lipopeptide surfactant fermented broth into a complex microbial preparation has not been reported in the literature so far.