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Polyporus umbellatus

A technology of Polyporus and strains, applied in horticultural methods, botanical equipment and methods, fungi, etc., to achieve the effect of extensive management and simple cultivation

Inactive Publication Date: 2004-08-18
刘德印
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It fundamentally solves the problem of the provenance of the artificial cultivation and breeding of Polyporus chinensis, so as to achieve the purpose of artificial breeding and cultivation of Polyporus umbilicalis, protect the ecological environment, increase the output of Chinese medicinal materials of Polyporus chinensis, and meet the needs of the medicinal market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Cultivation of mother species:

[0037] The medium formula is:

[0038] Potato 200g VB12 15mg

[0039] Agar 20g Diammonium 5g

[0040] 50% glucose 40ml 10% barley maltose infiltration solution 100ml

[0041] VB6 10mg Water 1000g

[0042] VB9 12mg pH 6-6.5

[0043] Production method: Potato peeled and sliced ​​boiled liquid filtered juice, agar was dissolved in the potato filtrate, 50% glucose was directly added to the upper liquid, VB6, VB9, VB12 were ground and dissolved in the upper liquid, diammonium was dissolved in the upper liquid, 10 The % barley maltose invading solution is directly added to the upper solution to make a mixed solution of the above ingredients;

[0044] Cultivation method: fill the prepared mixed solution into 1 / 5 of the test tube, seal it with cotton wool, put it in a 1.5kg / cm2 pressure cooker for sterilization for 25-30 minutes, stop the temperature supply and take out the test tube after the pointer of the pressure cooker returns to ze...

Embodiment 2

[0059] 1, mother species cultivation: same as embodiment 1.

[0060] 2, original seed culture: same as embodiment 1.

[0061] 3, cultivation of cultivars: same as embodiment 1.

[0062] 4. Cultivation method:

[0063] Humic plant embedding method: collect all kinds of humic plants, such as tree branches, stems, leaves, herbaceous plant stems, diameters, leaf shells and other dry materials can be used as humus base materials, using lamination, hole sowing, and spreading methods Crush the strains and embed them in the humus base material.

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Abstract

The Grifola umbellate seed is prepared with wild Grifola umbellate sclerotium as gene source and through artificial tissue separation, gene transferring, purification and copying, and is one kind of artificially cultured polyporoid seed. It is cultured in the mother seed culture medium with potato, agar and glucose as main components and expanding culture medium with sawdust, wheat bran, rice bran, rice husk as main material and cultivated in basswood or humic matter. The Grifola umbellate seed is synchronous one, that is, while the hypha obtains nutrients, the sporophore is formed instantaneously without needing other fungi to assist. It is easy to culture, so that the present invention makes it possible to provide Grifola umbellate to meet the requirement of pharmaceutical industry.

Description

technical field [0001] The invention relates to a strain of polyporus "Guo Shou No. 1" and a cultivation method thereof. Background technique [0002] Polyporus umbellata (Grifola umbellata) is a traditional Chinese medicinal material belonging to the family Polyporaceae and the genus Polyporus. Wild Polyporus umbellata resources are on the verge of extinction. How to artificially propagate and cultivate Polyporus umbellata to protect the ecological environment and increase the output of Polyporus umbellata Chinese medicinal materials, Satisfying the demand of the pharmaceutical market has become an urgent problem to be solved. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a strain of Polyporus chinensis "Guo Shou No. 1" and its cultivation method and cultivation method. Through artificial tissue separation, gene transfer, purification and replication, a strain of Polyporus spp. is artificially cultivated. Fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00C12N1/14
Inventor 刘德印
Owner 刘德印
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