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Bactericide bandung mycin and its upplication

A technology of Wanlongmycin and fungicides, which is applied in the field of plant protection and pesticides, can solve the problems of high price and drug resistance, and achieve the effects of easy preparation, easy operation, and no environmental pollution

Inactive Publication Date: 2004-07-21
PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and control of plant oomycete diseases mainly relies on chemical control. The commonly used fungicides are mainly imported Ruifu mold, antivirus alum and domestic metalaxyl and other drugs. These agents are expensive and have been used for many years. Some areas have developed resistance Medicinal properties

Method used

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  • Bactericide bandung mycin and its upplication
  • Bactericide bandung mycin and its upplication
  • Bactericide bandung mycin and its upplication

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The fungicide Wanlongmycin was prepared by culturing the strain GAAS2507 in a fermentation medium. Thaw 1 ml of frozen GAAS2507 nutrient medium, inoculate into 100 ml of nutrient medium in a 500 ml Erlenmeyer flask, and grow for 48 hours at 32° C. and shaking at 250 rpm.

[0024] Table 2 Nutrient medium components

[0025] Ingredient name Content (%)

[0026] Glucose 3

[0027] corn syrup 1.5

[0028] Soya bean cake flour 1.5

[0029] starch 1

[0030] NaCl 0.5

[0031] (NH 4 ) 2 SO 4 0.5

[0032] CaCO 3 0.5

[0033] pH 7.2

[0034] Take 3500ml of the mature first-stage vegetative culture to inoculate the secondary vegetative culture of 35L of nutrient medium in a 50L stirred fermenter, cultivate it at 32°C and 300rpm for 48 hours, and inoculate the mature strain into 500L In the agitated fermenter of , the fermentation culture was maintaine...

Embodiment 2

[0036] The present invention makes it possible to follow the production of the fungicide vanromycin during fermentation by testing extracts of the fermentation broth, for example by analyzing the extracts of the fermentation broth by bioassays.

[0037] After the fermentation is completed, the mycelium is separated from the 500L fermented product by plate and frame filter press, and the mycelium is leached with 1:2 acetone solution for 10 hours, the leaching solution is concentrated to remove acetone, and then extracted with ethyl acetate For the effective substance, the ethyl acetate extract is concentrated under reduced pressure to obtain an oily substance, the extracted concentrated solution is mixed with silica gel and passed through a column, and the silica gel column chromatography and concentration are performed with ethyl acetate and petroleum ether to obtain a pure oily substance.

Embodiment 3

[0039] A fungicide Wanlongmycin described in the present invention is suitable for preventing and treating bacterial and oomycete diseases, including the use of pathogenic bacteria inhibitory doses in the field. The effect of using the fungicide Wanlongmycin to control plant diseases is related to the frequency and method of use, as well as the species of the disease to be controlled. The present invention realizes the use of the preparation through the technical scheme disclosed in the Chinese invention patent application (the patent application number is 99117029.6).

[0040] The fungicide Wanlongmycin acetone solution was diluted twice with sterile water, and the inhibition curve and MIC against Bacillus subtilis and B. The results are shown in Tables 3 and 4.

[0041] Table 3 Inhibitory effect of fungicide Wanlongmycin on Bacillus subtilis

[0042] Concentration Inhibition zone radius (mm)

[0043] (μg / ml) 1 2 3 4 Average *

[0044] 1...

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Abstract

A disinfactant 'Wanlongmycin', C53H67N13O13S2 molecular weight 1157 is disclosed, which can be used to prevent the eliminate the bacterial and oomycetes' disease, such as rice streak disease, hoja blanca, frosty mildew, etc.

Description

technical field [0001] The invention relates to the technical fields of plant protection and pesticides, in particular to a fungicide Wanlongmycin and its application. Background technique [0002] Agricultural antibiotics have the characteristics of strong selectivity, high efficiency, and low toxicity. The research and development of antibiotics is a hot spot in pesticide research. Although there are many types of antibiotics reported to prevent and control plant diseases, there are few types that can be widely used in production. There is still a lack of high-efficiency and low-toxicity agricultural antibiotics for disease control. Oomycete disease refers to the disease caused by oomycete algal fungus infecting vegetables, fruit trees and other main economic crops, such as: melon blight, pepper blight, tomato late blight, potato late blight, cruciferous vegetable frost Mildew, melon downy mildew, litchi downy mildew, etc. These diseases develop ra...

Claims

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Application Information

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IPC IPC(8): A01N63/02C12N1/20
Inventor 谢双大林壁润江学斌周小毛沈会芳谢逢春杨丽梅
Owner PLANT PROTECTION RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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