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Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases

A technology of Helicobacter pylori and psoralen, which is applied in the direction of anti-infective drugs, anti-bacterial drugs, and medical preparations containing active ingredients, to achieve the effects of rich sources, convenient use, and simple preparation process

Inactive Publication Date: 2004-06-09
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, so far, there is no literature report on psoralen and its extracts against Helicobacter pylori infection and treatment of diseases related to Helicobacter pylori infection

Method used

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  • Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases
  • Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases
  • Application of Psoralca corylifolia in anti-infection of helicobacter pyloric and treating relative diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The psoralea seed powder is extracted with 95% ethanol by conventional extraction method to obtain the total extract. After the total extract was dissolved in water, it was extracted with petroleum ether and ethyl acetate respectively to obtain the petroleum ether part, ethyl acetate part and water-soluble part. The extraction rate of petroleum ether part and ethyl acetate part (fat-soluble part) is 9.0-15.0%.

Embodiment 2

[0020] Example 2 Anti-Helicobacter pylori effect in vitro

[0021] Add a certain amount of total psoralen extract and fat-soluble parts of the sample to be tested quantitatively to a certain amount of nutrient agar medium, mix well, pour into a sterile plate, and inoculate 107CFU / ml of Helicobacter pylori in logarithmic growth bacilli. After 5 days of microaerobic culture, they were transferred to drug-free medium by blind scraping, and microaerobic cultured at 37°C for 4-5 days. The growth of Helicobacter pylori was observed, and the minimum bactericidal concentrations (Minimum Bactericidal Concentrations, MBCs) were measured.

[0022] Table 1 Anti-Helicobacter pylori (Hp) effect of total psoralen extract and fat-soluble fraction in vitro

[0023] Minimum bactericidal concentrations (MBCs) for different Hp strains (μg / mL)

[0024] sample

[0025] Hp-Sydney strain 1(Hp, SS1) Hp-F44 Hp-Hudong

[0026] Positive control (metronidazole) 1.0 ...

Embodiment 3

[0030] Example 3 Pharmacological test in mice

[0031] Level 2 C 57 Fifty BL / 6 mice, weighing 20-25 g, were fed with a certain concentration of Helicobacter pylori (HpSS1) to achieve Hp colonization in the body. Mice colonized with Hp were randomly divided into blank control group, positive drug control group, high-dose group (600 mg / kg), middle-dose group (400 mg / kg) and low-dose group (200 mg / kg) of fat-soluble part of psoralen. Five groups, 10 in each group. The positive control group used triple therapy currently used in western medicine: omeprazole 5.2 mg / kg, clarithromycin 65 mg / kg and metronidazole 104 mg / kg. The blank control group was the corresponding excipients. The above experimental drugs were formulated into a certain concentration of suspension with 0.6% sodium carboxymethylcellulose-salad oil (1:1), fed through a gastric tube, twice a day (0.5 mL per mouse), and administered continuously for 14 days. sky. After 30 days of stopping the drug administration, ...

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Abstract

The present invention relates to Psoralca corylifolia and new application of its fat-soluble portion in medicine. They have obvious action for resisting helicobacter pylori (Hp), and can be used for curing the diseases of gastritis and peptide gastric ulcer, etc. resulted from helicobacter pylori infection, in particular, they are good therapeutic effect for curing active gastritis, gastric erosion, gastric ulcer and duodenal ulcer, and have the action for curing and preventing the diseases of gastric lymph tissue (MALT) lymphoma and early gastric cancer, moderate intestinal glands metaplasia or atypical hyperplasia.

Description

technical field [0001] The invention relates to a new application of traditional Chinese medicine, in particular to the application of psoraleae and its extract in resisting Helicobacter pylori infection and treating diseases related to Helicobacter pylori infection. Background technique [0002] Chronic active gastritis, gastric ulcer and duodenal ulcer, gastric MALT lymphoma and gastric cancer are diseases that seriously endanger human health and life. Medical research has confirmed that the above diseases are all related to Helicobacter pylori (Hp) infection. Data show that more than 50% of the population in the world is infected with Helicobacter pylori, especially in developing countries, where the infection rate of Helicobacter pylori in adults reaches 60-80%. In 1994, the International Agency for Research on Cancer (IARC) listed Hp as a Class I human carcinogen. At present, anti-endocrine drugs and antibiotics combined therapy are used to treat Hp infection at home a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/487A61P1/04A61P31/00A61P31/04A61P35/00
CPCA61K36/487A61P1/04A61P31/00A61P31/04A61P35/00
Inventor 岳建民樊成奇董蕾吴艳
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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