Method for quantitatively measuring tanshinonic acid B in red sage root

A technology for quantitative determination of salvianolic acid, which is applied in the field of quantitative determination of active ingredients in traditional Chinese medicine, can solve the problems of cumbersome pretreatment process, unstable chemical structure, poor operability, etc., and achieve good extraction recovery effect

Inactive Publication Date: 2006-07-12
CENT HOSPITAL XUHUI DISTRICT SHANGHAI CITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research results have shown that the chemical structure of salvianolic acid B itself as a strong antioxidant is not very stable. Under the conditions of heating, light, humidity and exposure to air, salvianolic acid B will Significant degradation occurs and the influence of temperature is particularly prominent
In the above-mentioned literature method, the sample needs to experience high temperature for a long time, which affects the reliability of the measurement results; and its pretreatment process is relatively cumbersome and the operability is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Chromatographic conditions: Column: Discovery TM RP-Amide C 16 (250×4.6mm, 5μm); column temperature: room temperature; mobile phase: methanol: acetonitrile: water: formic acid (42:5:52:1); flow rate: 1ml / min; detection wavelength: 285±5nm; injection volume : 20 μl. Under these chromatographic conditions, the peak eluting time of salvianolic acid B is about 12 minutes, and the sample column efficiency is better than 3000.

[0014] Preparation of reference substance solution: Take an appropriate amount of salvianolic acid B magnesium salt reference substance, accurately weigh it, add water to make a solution containing 0.1 mg of salvianolic acid B per 1 ml, and use it as a reference stock solution of salvianolic acid B, and accurately draw 5 ml of the stock solution Put it in a 10ml brown measuring bottle, add water to dilute to the mark, shake well, and use it as the salvianolic acid B reference solution. Both the stock solution and the reference solution must be fil...

Embodiment 2

[0018] Chromatographic conditions: Column: Discovery TM RP-Amide C 16 (250×4.6mm, 5μm); column temperature: room temperature; mobile phase: methanol: acetonitrile: water: formic acid (42:5:52:1); flow rate: 1ml / min; detection wavelength: 285±5nm; injection volume : 20 μl. Under these chromatographic conditions, the peak eluting time of salvianolic acid B is about 12 minutes, and the sample column efficiency is better than 3000.

[0019] Preparation of reference substance solution: Take an appropriate amount of salvianolic acid B magnesium salt reference substance, accurately weigh it, add water to make a solution containing 0.1 mg of salvianolic acid B per 1 ml, and use it as a reference stock solution of salvianolic acid B, and accurately draw 5 ml of the stock solution Put it in a 10ml brown measuring bottle, add water to dilute to the mark, shake well, and use it as the salvianolic acid B reference solution. Both the stock solution and the reference solution must be fil...

Embodiment 3

[0024] Chromatographic conditions: Column: Diamonsil TM C 18 (250×4.6mm, 5 μm); column temperature: room temperature; mobile phase: acetonitrile: water: formic acid (37:62:1); flow rate: 1ml / min; detection wavelength: 285±5nm; injection volume: 20 μl. Under these chromatographic conditions, the peak eluting time of salvianolic acid B is about 12 minutes, and the sample column efficiency is better than 2000.

[0025] Preparation of reference substance solution: Take an appropriate amount of salvianolic acid B magnesium salt reference substance, accurately weigh it, add water to make a solution containing 0.1 mg of salvianolic acid B per 1 ml, and use it as a reference stock solution of salvianolic acid B, and accurately draw 5 ml of the stock solution Put it in a 10ml brown measuring bottle, add water to dilute to the mark, shake well, and use it as the salvianolic acid B reference solution. Both the stock solution and the reference solution must be filled with nitrogen and ...

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Abstract

A process for quantitatively detecting the tanshinolic acid B from red sage root, its solid preparation, or its injection is characterized by that the ultrasonic treating-water extraction method is used for its pre-treating, the reverse-phase chromatography, where the C16 or C18 bonded-phase silica gel is used as fixed phase and the methanol-acetonitrile-water-formic acid (trifluoro acetic acid) or acetonitrile-water-formic acid (trifluoroacetic acid) is used as flowing phase, is used for separation, and the wavelength for ultraviolet detection is 285+ / -5 nm.

Description

technical field [0001] The invention relates to a method for quantitative determination of effective components of traditional Chinese medicines, more specifically a method for quantitative determination of salvianolic acid B in salvia miltiorrhiza crude drugs and preparations. Background technique [0002] Salvianolic acid B is a water-soluble compound present in various plants such as Salvia miltiorrhiza Bge. and Labiatae Salvia (S.Bowleyana Dunn). Phenolic acid compounds. In recent years, salvianolic acid B has been found to have a strong antioxidant effect (about 1000 times that of vitamin E) and the effect of scavenging oxygen free radicals. The lesions of tissues and organs all have obvious protective effect, which has been paid more and more attention by people. Due to the wide range of sources of traditional Chinese medicine, Danshen is no exception. The sources of crude drugs currently on the market are complex, and there are many varieties of Danshen preparation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/04G01N30/40G01N30/22
Inventor 张慧朱大元余琛蒋福详贾晶莹
Owner CENT HOSPITAL XUHUI DISTRICT SHANGHAI CITY
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