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Self-assembled terbium-cysteine nanoparticles as well as preparation method and application thereof

A technology of cysteine ​​and nanoparticles, applied in the field of biomedicine and medical evaluation, can solve problems such as models that are not suitable for Tb biological effects, and achieve the effect of promoting bone tissue repair and high biological safety

Pending Publication Date: 2022-08-02
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, TbCl 3 Is water soluble and not suitable as a model for studying the biological effects of Tb in vivo

Method used

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  • Self-assembled terbium-cysteine nanoparticles as well as preparation method and application thereof
  • Self-assembled terbium-cysteine nanoparticles as well as preparation method and application thereof
  • Self-assembled terbium-cysteine nanoparticles as well as preparation method and application thereof

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preparation example Construction

[0037] In view of this, in a typical embodiment of the present invention, a preparation method of self-assembled terbium-cysteine ​​nanoparticles is provided, the preparation method comprising: adding a terbium salt solution to a cysteine-containing alkaline solution Stir in the solution.

[0038] In yet another specific embodiment of the present invention, the molar ratio of terbium salt to cysteine ​​is 0.1-10:1, further 0.5-5:1, preferably 1:1; by controlling the molar ratio of terbium salt and cysteine The molar ratio is favorable for the subsequent self-assembly of terbium-cysteine ​​nanoparticles.

[0039] The terbium salt can be any water-soluble terbium salt, and in a specific embodiment of the present invention, the terbium salt is terbium nitrate (Tb(NO) 3 ) 3 ·5H 2 O); More specifically, the terbium nitrate concentration in the terbium nitrate aqueous solution is 0.1-0.5mmol / mL, preferably 0.2mmol / mL;

[0040] The cysteine-containing alkaline solution is a cyste...

Embodiment

[0064] 1. Materials and methods

[0065] 1.1 Experimental animals

[0066]Four Wistar male rats aged 4w, weighing 80-120 g, and 24 male Wistar rats aged 8w, weighing 200-250 g, were purchased from Beijing Speifu Biotechnology Co., Ltd.

[0067] 1.2 Experimental steps and methods

[0068] 1.2.1 Synthesis and characterization of Tb-Cys NPs

[0069] 1.2.1.1 Synthesis of Tb-Cys NPs

[0070] Dissolve 2 mmol of NaOH and 1 mmol of Cys in 5 mL of deionized water, mix them after they are completely dissolved, and stir evenly, and then mix Tb(NO) into 5 mL of deionized water. 3 ) 3 ·5H 2 O (5 mL, 1 mmol) aqueous solution was added dropwise to the mixed solution of NaOH and Cys, and stirred for 5 min to form Tb-Cys NPs. After the reaction, the precipitate was collected by centrifugation (8000 rpm, 5 min), then washed three times with deionized water and absolute ethanol, and the washed Tb-Cys NPs were stored in absolute ethanol and placed at 4 °C. for use in subsequent experiments...

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Abstract

The invention provides self-assembled terbium-cysteine nanoparticles and a preparation method and application thereof, belongs to the technical field of biomedicine and medicine evaluation, and aims to evaluate potential influence of Tb on bone tissues and long-term safety of Tb in vivo by taking Tb-Cys NPs as a model. Results prove that the Tb-Cys NPs has high cytocompatibility, osteogenesis and blood vessel differentiation of BMSCs can be promoted through complement / blood coagulation cascade and a cell cycle signal channel respectively, and meanwhile, the influence of Cys on cell behaviors is eliminated. Importantly, Tb-Cys NPs promotes osteogenesis and angiogenesis and induces efficient bone repair in vivo. Therefore, the Tb-Cys NPs not only can be used as a suitable model for Tb biosecurity evaluation, but also can be used as a regulatory factor, and has great potential in the aspect of promoting efficient bone repair.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and medical evaluation, and in particular relates to a self-assembled terbium-cysteine ​​nanoparticle and a preparation method and application thereof. Background technique [0002] The disclosure of information in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Rare earth elements (REEs) have been widely used in biomedicine due to their unique properties. REEs are often doped into host materials and exhibit different colors of fluorescence for biological imaging and tracking. In addition, REEs-based nanomaterials have been developed in recent years for optogenetics, including activation of neurons to trigger brain memory, mammalian near-infrared...

Claims

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Application Information

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IPC IPC(8): C07F5/00C09K11/06B82Y20/00B82Y40/00A61K33/24A61K9/14A61P19/08B82Y5/00
CPCC07F5/003C09K11/06B82Y20/00B82Y40/00A61K33/24A61K9/14A61P19/08B82Y5/00C09K2211/182
Inventor 葛少华马保金刘紫奇
Owner SHANDONG UNIV
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