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Montmorillonite nano-enzyme with high peroxidase-like activity and preparation method thereof

A peroxidase and montmorillonite technology, which is applied in the field of montmorillonite nanozyme and its preparation, can solve the problems of limiting the application of clay minerals, low enzyme-like activity, etc., and achieves excellent peroxidase-like catalytic activity, preparation Simple process, strong operability effect

Active Publication Date: 2022-07-08
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently known minerals with enzyme-like activity are mainly metallic ores with high iron content, while non-metallic minerals such as clay minerals have low enzyme-like activity due to low iron content, which limits the application of clay minerals

Method used

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  • Montmorillonite nano-enzyme with high peroxidase-like activity and preparation method thereof
  • Montmorillonite nano-enzyme with high peroxidase-like activity and preparation method thereof
  • Montmorillonite nano-enzyme with high peroxidase-like activity and preparation method thereof

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Effect test

Embodiment 1

[0021] A preparation method of a montmorillonite nanozyme with high peroxidase-like activity is specifically completed according to the following steps: mixing 3 g of iron-containing montmorillonite and 15 g of sodium nitrate, fully grinding in a mortar to make the mixture uniform, and then mixing the mixture Go to the crucible. The crucible was put into a muffle furnace, the reaction time was set to 4h, the temperature was set to 350°C, and the heating rate was 10° / min. After the reaction, the crucible was taken out and cooled to room temperature. The obtained solid is washed with deionized water for several times to remove sodium nitrate, and finally centrifuged to obtain the montmorillonite nanozyme precipitate, which is freeze-dried to obtain the montmorillonite nanozyme for use.

Embodiment 2

[0023] The sodium nitrate in Example 1 was replaced with lithium nitrate, and the parameters of other steps were unchanged.

Embodiment 3

[0025] Sodium nitrate was replaced with potassium nitrate in Example 1, and the parameters of other steps were unchanged.

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Abstract

The invention discloses a montmorillonite nano-enzyme with high peroxidase-like activity and a preparation method of the montmorillonite nano-enzyme. According to the preparation method, nitrate is taken as molten salt, iron-containing montmorillonite powder is treated by adopting a molten salt method, and the montmorillonite nano-enzyme with high peroxidase-like activity is obtained. According to the method, the natural clay mineral iron-containing montmorillonite which is rich in reserves can be used as a raw material, nitrate is used as molten salt, and the iron-containing montmorillonite is stripped by adopting a molten salt method, so that the peroxidase-like activity of the montmorillonite is improved while the microstructure of the montmorillonite is kept; the prepared montmorillonite nano-enzyme shows excellent peroxidase-like catalytic activity in normal saline, the dependence of the peroxidase-like activity of the conventional nano-enzyme on an acid environment is broken through, high-value utilization of montmorillonite mineral resources is facilitated, only two raw materials are used, montmorillonite is rich in reserve and low in price, and the method is suitable for industrial production. The preparation process is simple, the operability is high, large-scale production and preparation are easy, and the method is environment-friendly.

Description

technical field [0001] The invention relates to the technical field of mineral modification, in particular to a montmorillonite nanozyme with high peroxidase-like activity and a preparation method thereof. Background technique [0002] Some natural minerals have been confirmed to have enzyme-like activity, such as ferrihydrite, magnetite, hematite, etc. These minerals are widely distributed in the atmosphere, ocean, water, soil and other ecological environments. Mineral nanozymes have the advantages of simple preparation process, easy large-scale preparation, stable properties, strong tolerance to external environment, and low storage cost. Currently known minerals with enzyme-like activity are mainly metal ores with high iron content, while non-metallic minerals such as clay minerals have low enzyme-like activity due to low iron content, which limits the application of clay minerals. SUMMARY OF THE INVENTION [0003] The purpose of the present invention is to provide a m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/40B82Y40/00
CPCC01B33/40B82Y40/00
Inventor 杨华明汪浩
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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